BISC110/F09:Schedule

BISC110 F09 Lab Calendar

Monday	Tuesday	Wednesday	Thursday	Friday
Sept. 7 Labor Day Holiday	Sept. 8 Lab 1	Sept. 9 Lab 1	Sept. 10 Lab 1	Sept. 11 Lab 1
Sept. 14 Lab 1	Sept. 15 Lab 2	Sept. 16 Lab 2	Sept. 17 Lab 2	Sept. 18 Lab 2
Sept. 21 Lab 2	Sept. 22 Lab 3	Sept. 23 Lab 3	Sept. 24 Lab 3	Sept. 25 Lab 3
Sept. 28 Lab 3	Sept. 29 Lab 4	Sept. 30 Lab 4	Oct. 1 Lab 4	Oct. 2 Lab 4 ||
Oct. 5 Lab 4	Oct. 6 Lab 5	Oct. 7 Lab 5	Oct. 8 Lab 5	Oct. 9 Lab 5
Oct. 12 Fall Break No Lab	Oct. 13 No Lab	Oct. 14 Wed. follows Mon. Schedule Lab 5	Oct. 15 No Lab	Oct. 16 No Lab
Oct. 19 Lab 6	Oct. 20 Lab 6	Oct. 21 Lab 6	Oct. 22 Lab 6	Oct. 23 Lab 6
Oct. 26 Lab 7	Oct. 27 Lab 7	Oct. 28 Lab 7	Oct. 29 Lab 7	Oct. 30 Lab 7
Nov. 2 Lab 8	Nov. 3 Tanner Conference No Lab	Nov. 4 Lab 8	Nov. 5 Lab 8	Nov. 6 Lab 8
Nov. 9 No Lab	Nov. 10 Lab 8	Nov. 11 Lab 9	Nov. 12 Lab 9	Nov. 13 Lab 9
Nov. 16 Lab 9	Nov. 17 Lab 9	Nov. 18 Lab 10	Nov. 19 Lab 10	Nov. 20 Lab 10
Nov. 23 Lab 10	Nov. 24 Lab 10	Nov. 25 No Lab	Nov. 26 Thanksgiving Holiday	Nov. 27 Thanksgiving Holiday No Lab
Nov. 30 Lab 11	Dec. 1 Lab 11	Dec. 2 Lab 11	Dec. 3 Lab 11	Dec. 4 Lab 11
Dec. 7 Lab 12	Dec. 8 Lab 12	Dec. 9 Lab 12	Dec. 10 Lab 12	Dec. 11 Lab 12

Schedule of Experiments

Section	Lab #	Activity
1	1-4	Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Phagocytosis Asssay; Designing Experiments
2	5-7	Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis; Case study, ethics
3	8-10	Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Statistical parameters; Hill Reaction: Self-designed investigation of factors affecting photosystems
4	11	Regulation of gene expression: Enzyme assay
Lab Practical	12	Hands-on skills assessment:

Lab Graded Assignments

BISC110 F09 Lab Planner

Section	Lab #	DATE	LAB DESCRIPTION	Assignment/Notes
1	1	Tues. 9/9- Mon. 9/14	Part 1: Metric Measurements- Practice conversions; Part 2: Basic Laboratory Equipment- Practice pipetting; Part 3: Making Solutions; Part 4: Microscopy; Part 5: Making Dilutions	Homework: Practice Problems due next lab Dilution Help Tutorials
1	2	Tue Feb 13	Agarose gel electrophoresis	PDF of class notes
1	3	Thur Feb 15	DNA ligation and bacterial transformation
1	4	Thur Feb 22	Examine candidate clones	guest lecture from Harlan Breindel
1	5	Tue Feb 27	Western analysis	PDF of class discussion slides
1	6	Thur Mar 1	Probe western	first draft of your writing assignment due before lab, PDF of class slides
2	1	Tue Mar 6	Start-up signal measurement
2	2	Thur Mar 8	Measuring calcium in vitro
2	3	Tue Mar 13	Lipofection
2	4	Thur Mar 15	Measuring calcium in vivo
2	5	Tue Mar 20	Calcium signaling in vivo
2	6	Thur Mar 22	Student presentations
3	1	Tue Apr 3	Start-up expression engineering	PDF of M3D1 lecture slides
3	2	Thur Apr 5	Yeast transformation	PDF of M3D2 lecture slides
3	3	Tue Apr 10	Colony PCR	guest lecture: Susan Ruff and her slides are here
3	4	Thur Apr 12	Screen for phenotypes, isolate RNA	PDF of M3D4 lecture slides
3	5	Thur Apr 19	cDNA synthesis and microarray	PDF of M3D5 lecture slides
3	6	Tue Apr 24	Microarray data analysis	guest lecture: Rebecca Fry and her slides are here
4	1	Thur Apr 26	Screening library
4	2	Tue May 1	Optimizing panning	first draft of your writing assignment due Monday April 30th by 9AM
4	3	Thur May 3	Rescreening gold binders
4	4	Tue May 8	PCR of gold binding candidates	Mod 3 lab reports returned for revision
4	5	Thur May 10	Student presentations
4	6	Tue May 15	Analyze sequence data	Revised Mod 3 writing assignment due before lab no lecture today