BISC110/F09:Series3

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'''In this semester long experiment you will:'''  <br>       
'''In this semester long experiment you will:'''  <br>       
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#use the Agrobacterium system to introduce a foreign gene, the β -glucuronidase gene (gusA) of E. coli, into the cells of tobacco<br>
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#<br>
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#take advantage of the totipotency of plant cells and use these transformed cells to regenerate genetically engineered plants<br>
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#<br>
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#confirm that the regenerated plants are transformed by<br>
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#<br>
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##measuring the activity of the introduced enzyme, β–glucuronidase (GUS) spectrophotometrically and histochemically
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##PCR to test directly for the presence of the introduced genes and  <br>
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##<br>
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#become adept at sterile technique.
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#  
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! Concepts !! Genes/Organisms !! Techniques/Skills
! Concepts !! Genes/Organisms !! Techniques/Skills
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| Effect of random gene insertion in a large genome
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| Bacterial pathogen: ''Agrobacterium tumefaciens'' || Aseptic tissue culture
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| Plant totipotency
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|Tobacco plant: ''Nicotiana tobacum''
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|DNA Extraction
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|Regulation of gene expression
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|gusA gene from ''E. coli''
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|PCR
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|Genetic Engineering and creation of genetically modified organism
 
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|Agarose gel electrophoresis
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|Phenotypic Selection
 
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|Transfection & selection by antibiotic resistance;
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Enzyme function assays: colorimetric & histochemical
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Revision as of 13:42, 8 June 2009

Wellesley College-BISC110 Introduction to Cell Biology-Fall 2009

At the end of this three week series on photosynthesis (Labs 8-10) you will have mastered the following skills and concepts.

Series Three- Photosynthesis-Objectives:

In this semester long experiment you will:






Concepts Genes/Organisms Techniques/Skills


Experiment 8: Photosynthetic Pigments
Experiment 9: The Hill Reaction
Experiment 10: Variables Affecting the Hill Reaction

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