BISC110/F09:Series3

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(Series Three Photosynthesis (Labs 8-10) Objectives:)
 
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At the end of this three week series on photosynthesis (Labs 8-10) you will have mastered the following skills and concepts.
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=='''Series 3 - Photosynthesis (Labs 8-10) Objectives:'''==
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=='''Series Three- Photosynthesis-Objectives:'''==
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'''In this series of three weeks of experiments you will learn:'''   <br>     
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# How photosynthetic pigments are harvested and used ''in vivo'' in converting light energy to chemical energy<br>
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# How to extract, separate, measure, and analyze photosynthetic pigments <br>
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# How to use an artificial electron acceptor to measure an aspect of photosynthesis ''in vitro'' <br>
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# How to investigate a variable that might affect the rate of electron transport in photosynthesis
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# How to write an original scientific research report<br>
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'''In this semester long experiment you will:'''  <br>     
 
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#use the Agrobacterium system to introduce a foreign gene, the β -glucuronidase gene (gusA) of E. coli, into the cells of tobacco<br>
 
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#take advantage of the totipotency of plant cells and use these transformed cells to regenerate genetically engineered plants<br>
 
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#confirm that the regenerated plants are transformed by<br>
 
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##measuring the activity of the introduced enzyme, β–glucuronidase (GUS) spectrophotometrically and histochemically
 
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##PCR to test directly for the presence of the introduced genes and  <br>
 
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#become adept at sterile technique.
 
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[[BISC110: Series 3 Experiment 8 Photosynthetic Pigments | Lab 8: Photosynthetic Pigments]]<br>
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[[BISC110: Series 3 Experiment 9 Hill Reaction | Lab 9: The Hill Reaction]]<br>
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{| border="1"
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[[BISC110: Series 3 Experiment 10 Hill Reaction Variables | Lab 10: Variables Affecting the Hill Reaction]]<br>
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<br>
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! Concepts !! Genes/Organisms !! Techniques/Skills
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| Effect of random gene insertion in a large genome
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| Bacterial pathogen:  ''Agrobacterium tumefaciens'' || Aseptic tissue culture
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| Plant totipotency
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|Tobacco plant: ''Nicotiana tobacum''
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|DNA Extraction
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|Regulation of gene expression
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|gusA gene from ''E. coli''
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|PCR
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|Genetic Engineering and creation of genetically modified organism
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|Agarose gel electrophoresis
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|Phenotypic Selection
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|Transfection & selection by antibiotic resistance;
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Enzyme function assays: colorimetric & histochemical
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|}
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[[BISC110: Series 3 Experiment 8 Photosynthetic Pigments | Experiment 8: Photosynthetic Pigments]]<br>
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[[BISC110: Series 3 Experiment 9 Hill Reaction | Experiment 9: The Hill Reaction]]<br>
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[[BISC110: Series 3 Experiment 10 Hill Reaction Variables | Experiment 10: Variables Affecting the Hill Reaction]]<br>
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Current revision

Wellesley College-BISC110 Introduction to Cell Biology-Fall 2009

Series 3 - Photosynthesis (Labs 8-10) Objectives:

In this series of three weeks of experiments you will learn:

  1. How photosynthetic pigments are harvested and used in vivo in converting light energy to chemical energy
  2. How to extract, separate, measure, and analyze photosynthetic pigments
  3. How to use an artificial electron acceptor to measure an aspect of photosynthesis in vitro
  4. How to investigate a variable that might affect the rate of electron transport in photosynthesis
  5. How to write an original scientific research report


Lab 8: Photosynthetic Pigments
Lab 9: The Hill Reaction
Lab 10: Variables Affecting the Hill Reaction


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