BISC110/F10:Schedule: Difference between revisions
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
||
(28 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
{{Template:BISC110/F10}} | {{Template:BISC110/F10}} | ||
<div style="padding: 10px; width: 720px; border: 5px solid # | <div style="padding: 10px; width: 720px; border: 5px solid #2171B7;"> | ||
<!-- Replace everything below this line with information about the people involved in your course --> | <!-- Replace everything below this line with information about the people involved in your course --> | ||
== | ==BISC110/112 F10 Lab Calendar== | ||
<br> | <br> | ||
Line 86: | Line 86: | ||
== ''' Assignments ''' == | == ''' Assignments ''' == | ||
==BISC110 Assignments/ Weekly Lab Planner== | ==BISC110/112 Assignments/ Weekly Lab Planner== | ||
{| border=1px | {| border=1px | ||
Line 99: | Line 99: | ||
| 1 | | 1 | ||
| 1 | | 1 | ||
| | | Sept. 7 to<br> Sept. 13 | ||
| Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of ''Tetrahymena pyriformis''<br> | | Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br> | ||
| '''Homework''': Familiarize yourself with the wiki | | '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/F10:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br> | ||
Read about the process of endocytosis in your textbook | Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena'' from the following reference articles:<BR> Gronlien HK, Berg T, Lovlie AM.In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097; <BR>McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257. | ||
|-- | |-- | ||
| 1 | | 1 | ||
| 2 | | 2 | ||
| | | Sept.14 to<br> Sept. 20 | ||
| ''Tetrahymena'' Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. | | ''Tetrahymena'' Behavior: Phagocytosis; Observations;<BR> Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation;<BR> Effective Figure Design and Legends using the Gronlien article as a model. | ||
| '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends from your ''Tetrahymena'' experiment: one or more microphotographs of Tetrahymena and a graph or table of data. | | '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends<BR> from your ''Tetrahymena'' experiment:<BR> one or more microphotographs of Tetrahymena <BR>and a graph or table of data. | ||
|-- | |-- | ||
| 1 | | 1 | ||
| 3 | | 3 | ||
| | | Sept. 21 to<br> Sept. 27 | ||
| Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena''; Design your own experiment exploring inhibitors and ''Tetrahymena'' phagocytosis | | Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena'';<BR> Design your own experiment exploring <BR>inhibitors and ''Tetrahymena'' phagocytosis | ||
| '''Homework''': There are animated tutorials on dilution | | '''Homework''': There are animated tutorials on dilution<BR> available at the following link: [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4;<BR> have it approved by your lab instructor;<br> Write a results section of a scientific report <BR>on your findings in the ''Tetrahymena'' toxicity experiment <BR>including a narrative portion and integrated figures with legends;<br> | ||
Work the dilution problems found at [[Media: | Work the dilution problems found at<BR> [[Media: Lab_3_110_Dilution_Problems.doc]] and hand them in in Lab 4. | ||
|-- | |-- | ||
| 1 | | 1 | ||
| 4 | | 4 | ||
| | | Sept. 28 to <br> Oct. 4 | ||
| Student Designed Experiments: Potential Inhibitors of Vacuole Formation; | | Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation; | ||
| '''Homework''': 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion [[http://www.wellesley.edu/Library/ Link to Wellesley College Library]] | | '''Homework''': 5 minute oral presentation in PowerPoint of your data,<BR> including a figure and figure legend, <BR>summarizing your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>[[http://www.wellesley.edu/Library/ Link to Wellesley College Library]] | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
| | | Oct. 5 to <br> Oct. 12 | ||
| Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop; | | Scientific Writing Workshop with Journal Article Discussion;<BR> Science Writing Workshop; | ||
| '''Homework''': Partial | | '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/F10:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at:<BR> [http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br> | ||
Have Lab notebooks ready to be turned in for inspection after Lab 6. | Have Lab notebooks ready to be turned in<BR> for inspection after Lab 6. | ||
<br> Read Lab 6 (first lab in Series 2, Genetics) | <br> Read Lab 6 (first lab in Series 2, Genetics) | ||
<br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures. | <br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures. | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 6 | | 6 | ||
| | | Oct. 13 to<br> Oct. 19 | ||
| Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; | | Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR; | ||
| '''Homework''': Read the following background information on restriction enzymes found at: | | '''Homework''': Read the following background information<BR> on restriction enzymes found at:[http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf] | ||
and on gel electrophoresis at: | and on gel electrophoresis at:[http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf]; also | ||
Read the following background information on ethical issues in genetic testing: | Read the following background information<BR> on ethical issues in genetic testing: | ||
Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 and found at: | Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 and found at:[http://jmd.amjpathol.org/cgi/content/full/11/4/263 http://jmd.amjpathol.org/cgi/content/full/11/4/263<BR> | ||
PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in | PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in | ||
''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604] (in Policy Forum) found | ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)found | ||
at: | at: [http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf];<BR> | ||
AMA DTC testing policy recommendations at: | AMA DTC testing policy recommendations at:[http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156];<br> | ||
Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel; | Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; | ||
<br>Answer the questions found at the following downloadable link:[[Media: | <br>Answer the questions found at the following downloadable link:<BR>[[Media:Taster_DTC_questions110.doc]] to use as a template for your answers.''' | ||
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
Line 152: | Line 152: | ||
| 2 | | 2 | ||
| 7 | | 7 | ||
| | | Oct. 20 <br> Oct. 26 | ||
| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Determine PTC Taster Genotype; Scientific Writing Workshop | | Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ;<BR> Determine PTC Taster Genotype; Scientific Writing Workshop | ||
| '''Homework | | '''Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.<BR> Due at the beginning of LAB 8.'''<BR> For help, see the [[BISC110/F10:Resources | Resources]] <BR>section of this wiki. | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 8 | | 8 | ||
| | | Oct. 28 to<br> Nov. 3 | ||
| Photosynthesis 1: Photosynthetic pigments | | Photosynthesis 1: Photosynthetic pigments | ||
| '''Homework''': Prepare a full results section for the photosynthetic pigment identification experiment;<br> | | '''Homework''': Prepare a full results section<BR> for the photosynthetic pigment identification experiment;<br> | ||
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br> | In your lab notebook,<BR> prepare a flow chart for the Hill Reaction (Lab 9);<br> | ||
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br> | Review the mechanisms of electron transport<BR> and proton flow in photosynthesis (use your textbook).<br> | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 9 | | 9 | ||
| | | Nov. 4 to<br> Nov. 10 | ||
| Photosynthesis 2: Hill Reaction | | Photosynthesis 2: Hill Reaction | ||
| '''Homework''': Finalize your self-designed experiment testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | | '''Homework''': Finalize your self-designed experiment<BR> testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version<BR> of the Hill Reaction experiment that you did this week in Lab 9 | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 10 | | 10 | ||
| | | Nov.11 to <br> Nov. 17 | ||
| Photosynthesis 3: Investigation of variables affecting the Hill Reaction | | Photosynthesis 3: Investigation of variables affecting the Hill Reaction | ||
| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments with | | '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments <BR>(with the treatments on the same graph).See([[Appendix_E/F10 |Appendix E]]). <BR> Determine regression equations for the linear part<BR> of each data plots to get slope (m) values<BR> (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs) <BR>and plot those values against your variable (x-axis);<br> | ||
A full paper on your experiment | '''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11.'''<BR> Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/F10:Guidelines for writing a lab report in the form of a scientific paper | '''Guidelines for science writing''']]" <BR>information found in the [[BISC110/F10:Resources | Resources]]<BR> section of the wiki. | ||
|-- | |-- | ||
| 4 | | 4 | ||
| 11 | | 11 | ||
| | | Nov. 29 to Dec. 3 | ||
| Enzymology | | Enzymology | ||
| '''Homework | | '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12.'''<br> | ||
Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:[[Media: Lab_11_Questions_110.doc]] | Answer the following questions<BR> (5 points) and hand them in to your instructor <BR>at the beginning of Lab 12. <BR>Download a Word document form of these questions here:[[Media: Lab_11_Questions_110.doc]] | ||
<br> Make sure Lab Notebook is ready to turn in for grading | <br> Make sure Lab Notebook is ready to turn in for grading | ||
<br> | <br> | ||
Line 193: | Line 193: | ||
| Skills Assessment | | Skills Assessment | ||
| 12 | | 12 | ||
| | | Dec. 6 to <br> Dec. 10 | ||
| Lab Practical; <br>Turn in Lab Notebook | | '''Lab Practical'''; <br>Turn in Lab Notebook | ||
| End of lab | | End of lab | ||
|-- | |-- |
Latest revision as of 06:09, 6 September 2010
BISC110/112 F10 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday | |
---|---|---|---|---|---|
Sept. 6 Labor Day Holiday |
Sept. 7 Lab 1 |
Sept. 8 Lab 1 |
Sept. 9 Lab 1 |
Sept. 10 Lab 1 |
|
Sept. 13 Lab 1 |
Sept. 14 Lab 2 |
Sept. 15 Lab 2 |
Sept. 16 Lab 2 |
Sept. 17 Lab 2 |
|
Sept. 20 Lab 2 |
Sept. 21 Lab 3 |
Sept. 22 Lab 3 |
Sept. 23 Lab 3 |
Sept. 24 Lab 3 |
|
Sept. 27 Lab 3 |
Sept. 28 Lab 4 |
Sept. 29 Lab 4 |
Sept. 30 Lab 4 |
Oct. 1 Lab 4 |
|
Oct. 4 Lab 4 |
Oct. 5 Lab 5 |
Oct. 6 Lab 5 |
Oct. 7 Lab 5 |
Oct. 8 Lab 5 |
|
Oct. 11 Fall Break No Lab |
Oct. 12 MON. Sch. Lab 5 |
Oct. 13 Lab 6 |
Oct. 14 Lab 6 |
Oct. 15 Lab 6 |
|
Oct. 18 Lab 6 |
Oct. 19 Lab 6 |
Oct. 20 Lab 7 |
Oct. 21 Lab 7 |
Oct. 22 Lab 7 |
|
Oct. 25 Lab 7 |
Oct. 26 Lab 7 |
Oct. 27 Tanner Conf. No Lab |
Oct. 28 Lab 8 |
Oct. 29 Lab 8 |
|
Nov. 1 Lab 8 |
Nov. 2 Lab 8 |
Nov. 3 Lab 8 |
Nov. 4 Lab 9 |
Nov. 5 Lab 9 |
|
Nov. 8 Lab 9 |
Nov. 9 Lab 9 |
Nov. 10 Lab 9 |
Nov. 11 Lab 10 |
Nov. 12 Lab 10 |
|
Nov. 15 Lab 10 |
Nov. 16 Lab 10 |
Nov. 17 Lab 10 |
Nov. 18 NO Lab |
Nov. 19 No Lab |
|
Nov. 22 No Lab |
Nov. 23 No Lab |
Nov. 24 No Lab |
Nov. 25 Thanksgiving Holiday |
Nov. 26 Thanksgiving Holiday |
|
Nov. 29 Lab 11 |
Nov. 30 Lab 11 |
Dec. 1 Lab 11 |
Dec. 2 Lab 11 |
Dec. 3 Lab 11 |
|
Dec. 6 Lab 12 |
Dec. 7 Lab 12 |
Dec. 8 Lab 12 |
Dec. 9 Lab 12 |
Dec. 10 Lab 12 |
Schedule of Experiments
Section | Lab # | Activities |
---|---|---|
1 | 1-5 | Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing |
2 | 6-7 | Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis |
3 | 8-10 | Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems |
4 | 11 | Gene Regulation/Enzymology: Invertase enzyme assay |
Lab Practical | 12 | Hands-on skills assessment: |
Assignments
BISC110/112 Assignments/ Weekly Lab Planner
Series | Lab # | LAB DATES__ | LAB DESCRIPTION________ | Assignment/Notes | ||
1 | 1 | Sept. 7 to Sept. 13 |
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis |
Homework: Familiarize yourself with the wiki, particularly the information in the BISC110/F10:Resources section of the Lab wiki; Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_Problems110.doc; Read about the process of endocytosis in your textbook; | ||
1 | 2 | Sept.14 to Sept. 20 |
Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. |
Homework: Study dilution making for Lab 3; Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data. | ||
1 | 3 | Sept. 21 to Sept. 27 |
Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis |
Homework: There are animated tutorials on dilution available at the following link: Dilution Help Tutorials; Finalize your experimental design for Lab 4; have it approved by your lab instructor; Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends; Work the dilution problems found at | ||
1 | 4 | Sept. 28 to Oct. 4 |
Student Designed Experiments: Potential Inhibitors of Vacuole Formation; |
Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. Read assigned journal article for science writing discussion [Link to Wellesley College Library] | ||
2 | 5 | Oct. 5 to Oct. 12 |
Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop; |
Homework: Tetrahymena Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6 Title Page, Results, Discussion and References sections. Guidelines at: Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources. Have Lab notebooks ready to be turned in | ||
2 | 6 | Oct. 13 to Oct. 19 |
Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; |
Homework: Read the following background information on restriction enzymes found at:http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf and on gel electrophoresis at:http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf; also
Read the following background information | ||
2 | 7 | Oct. 20 Oct. 26 |
Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Determine PTC Taster Genotype; Scientific Writing Workshop |
Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study. Due at the beginning of LAB 8. For help, see the Resources section of this wiki. | ||
3 | 8 | Oct. 28 to Nov. 3 |
Photosynthesis 1: Photosynthetic pigments | Homework: Prepare a full results section for the photosynthetic pigment identification experiment; In your lab notebook, | ||
3 | 9 | Nov. 4 to Nov. 10 |
Photosynthesis 2: Hill Reaction | Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction; Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9 | ||
3 | 10 | Nov.11 to Nov. 17 |
Photosynthesis 3: Investigation of variables affecting the Hill Reaction | Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (with the treatments on the same graph).See(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis); A full paper on your experiment, in the form of a scientific research report, | ||
4 | 11 | Nov. 29 to Dec. 3 | Enzymology | Homework: Prepare for a Lab Practical Skills Assessment in lab 12. Answer the following questions | ||
Skills Assessment | 12 | Dec. 6 to Dec. 10 |
Lab Practical; Turn in Lab Notebook |
End of lab |
Lab Graded Assignments
Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
---|---|---|---|
1 | 2 | Practice Problems | 5 |
2 | 3 | Fig. Design (ungraded) | 0 |
3 | 4 | Dilution Problems (5); Fig.& Results (10) |
15 |
4 | 5 | Oral presentation: Results, original figure & legend; Journal article discussion |
10 |
5 | 6 | Lab notebook check (ungraded); Partial Scientific Paper(25) |
25 |
6 | 7 | SNP Taster Questions | 5 |
7 | Due for all students Fri. 4/2 |
Taster Scientific Paper (all but M&M) | 35 |
8 | 9 | Photosyn. Pigments Results | 10 |
9 | 10 | M&M (5pt); Finalize Self-Design Experiment Hill Rxn.(ungraded) |
5 |
10 | 11 | Full Scientific Paper Hill Rxn. | 35 |
11 | 12 | Lab Notebook Check (10) Concept Questions Enzymology/Gene Reg. (5) |
15 |
12 | 12 | Lab Practical:Hands-on skills assessment |
35 |
Other | - | Discretionary Points Preparation & Participation |
5 |