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| == '''BISC110/112 Assignments/ Weekly Lab Planner''' ==
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| {| border=1px
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| |'''Series'''
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| |'''Lab #'''
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| |'''LAB DATES'''
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| |'''LAB DESCRIPTION'''
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| |'''Assignment/Notes'''
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| |--
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| | colspan="6" |
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| |--
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| | 1
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| | 1
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| | 9/4-9/10<br>
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| | Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br>
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| | '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/F12:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br>
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| Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena''<BR> from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'',<BR> the non-selective phagocytosis seen in microstomes <BR>changes to a highly selective process in macrostomes.<BR> (2002), Journal of Experimental Biology 205, 2089-2097; <BR>
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| '''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin,<BR> and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
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| | 1
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| | 2
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| | 9/11-9/17<br>
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| | ''Tetrahymena'' Behavior: Phagocytosis; Observations; Data Analysis;<BR> Accuracy vs. Precision; Statistical Parameters;<BR> Data Presentation; Effective Figure Design and Legends<BR> using the Gronlien article as a model.
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| | '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends from your ''Tetrahymena'' experiment:<BR> one or more microphotographs of Tetrahymena and a graph or table of data. Generate a figure of means +/- standard deviations and a caption for the theoretical data table at the end of the Assignments section in lab 2.
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| | 1
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| | 3
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| | 9/8-9/24<br>
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| | Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena'';<BR> Design your own experiment exploring<BR> inhibitors and ''Tetrahymena'' phagocytosis
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| | '''Homework''': There are animated tutorials on dilution<BR> available at the following link:<BR> [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4; have it approved by your lab instructor;<br> Write a results section of a scientific report<BR> on your findings in the ''Tetrahymena'' toxicity experiment <BR>including a narrative portion and integrated figure(s) with legend(s);<br>
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| Solve the dilution problems found at<BR> [[Media: Lab_3_110_Dilution_Problems.doc]] and hand them in<BR> by the beginning of Lab 4.
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| | 1
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| | 4
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| | 9/25-10/1<br>
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| | Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation;
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| | '''Homework''': 5 minute oral presentation in PowerPoint™ of your data,<BR> including at least one figure with legend, of your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>'''* Gronlien, H.K., Berg, T., Lovlie, A.M.''' (2002).<BR> In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes<BR> to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097
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| | 1
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| | 5
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| | 10/2-10/15 <br>
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| | Scientific Writing Workshop with Journal Article Discussion
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| | '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/F12:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: <BR>[http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br>
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| Have Lab notebooks ready to be turned in for inspection after Lab 6.
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| <br> Read Lab 6 (first lab in Series 2, Genetics)
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| <br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
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| | 2
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| | 6
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| | 10/16-10/22<br>
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| | Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR;
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| | '''Homework''': Read the following background information on<BR> [http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf restriction enzymes] <BR>and on [http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf gel electrophoresis] <br>
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| Read the following background information on ethical issues in genetic testing:<br>
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| '''[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710700/?tool=pubmed Direct-to-Consumer Genetic Testing,'''<BR> Two Sides of the Coin] by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18. <BR>
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| [http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf PUBLIC HEALTH: <BR>A Case Study of Personalized Medicine] by S. H. Katsanis, G. Javitt, and K. Hudson,<BR> published in ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)<BR>
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| [http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br>
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| Draw the expected migration locations of the Fnu4H1 digested PCR product<BR> obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br>
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| Answer the questions found at:<BR>[[Media:Taster_DTC_questions110112_S12.doc]]<BR>to use as a template for your answers.'''
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| |--
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| | colspan="6" |
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| |--
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| | 2
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| | 7
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| | 10/23-10/29 <br>
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| | Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;<BR> Determine PTC Taster Genotype; Scientific Writing: <BR>Introduction & Discussion
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| | '''Homework:
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| Prepare draft figures/tables from the preliminary course data<BR> for the taster investigation paper and an skeleton outline<BR> of your introduction & discussion sections.<BR> (Refer to the handout on How to Prepare a Skeleton Outline in Sakai.)
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| |--
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| | 2 & 3
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| | 8
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| | 10/30-11/5<br>
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| | Data Analysis of Taster investigation; Science Writing Workshop:<BR> Effective Figure Design, Introduction, Discussion; Photosynthesis 1:<BR> Chlorophyll pigment investigation, Spectroscopy,<BR> The Beer-Lambert Law and Creating a Standard Curve
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| | '''Homework''': Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.'''<BR> For help, see the [[BISC110/F12:Resources | Resources]] section of this wiki.<br>
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| In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br>
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| Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br>
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| | 3
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| | 9
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| | 11/7-11/13<br>
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| | Photosynthesis 2: Hill Reaction
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| | '''Homework''': Finalize your self-designed experiment<BR> testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction<BR> experiment that you did this week in Lab 9
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| | 3
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| | 10
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| | 11/14-11/20<br>
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| | Photosynthesis 3: Investigation of variables affecting the Hill Reaction
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| | '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments (with the treatments on the same graph).<BR>See([[Appendix_E/F12 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots<BR> to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs)<BR> and plot those values against your variable (x-axis);<br>
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| '''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11 (or on day indicated in Lab Calendar).'''<BR> Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/F12:Guidelines for Science Writing | '''Guidelines for science writing''']]" information<BR> found in the [[BISC110/F12:Resources | Resources]] section of the wiki.
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| | 3
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| | 11
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| | 11/26-11/30<br>
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| | Science Writing Workshop<BR>Bring a draft of your Results & Discussion sections (5 points) <BR> (including figures/tables) of your photosynthesis paper
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| | '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12'''<br>
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| <br> Make sure Lab Notebook is ready to turn in for grading at the beginning of LAB 12
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| | Skills Assessment
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| | 12
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| | 12/3-12/7<br>
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| | '''Lab Practical'''; <br>Turn in Lab Notebook
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| | End of lab
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| |--
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| |}
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| <br>
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| =='''Lab Graded Assignments'''== | | =='''Lab Graded Assignments'''== |
