BISC110/F12:Schedule

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(BISC110/112 F12 Lab Calendar DRAFT)
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(Lab Graded Assignments)
 
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==<font color="red">BISC110/112 F12 Lab Calendar DRAFT</font color="red">==
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==BISC110/112 F12 Lab Calendar==
{| border="2"  
{| border="2"  
|+  
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|-
|-
| October 29 <br>'''Lab 7'''
| October 29 <br>'''Lab 7'''
-
| October 30 <br> '''Lab 8''' || October 31 <br> '''Lab 8''' || November 1 <br> '''Lab 8'''|| November 2 <br> '''Lab 8'''||   
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| October 30 <br>'''Tanner Conference <BR> No Classes''' || October 31 <br> '''Lab 8''' || November 1 <br> '''Lab 8'''|| November 2 <br> '''Lab 8'''||   
|-
|-
| November 5 <br> '''Lab 8'''
| November 5 <br> '''Lab 8'''
-
| November 6 <br>'''Tanner Conference <BR> No Classes''' || November 5 <br>'''Lab 9''' || November 6 <br>'''Lab 9''' || November 7 <br>'''Lab 9'''||   
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| November 6 <br>'''Lab 8''' || November 7 <br>'''Lab 9''' || November 8 <br>'''Lab 9''' || November 9 <br>'''Lab 9'''||   
|-
|-
| November 12<br> '''Lab 9'''
| November 12<br> '''Lab 9'''
-
| November 13 <br>'''Lab 9''' || November 9 <br>'''Lab 10''' || November 10 <br>'''Lab 10''' || November 11 <br>'''Lab 10'''||   
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| November 13 <br>'''Lab 9''' || November 14 <br>'''Lab 10''' || November 15 <br>'''Lab 10''' || November 16 <br>'''Lab 10'''||   
|-
|-
| November 19<br> '''Lab 10'''
| November 19<br> '''Lab 10'''
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|-
|-
| December 10 <br> '''NO LAB'''
| December 10 <br> '''NO LAB'''
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| December 11 <br> <br>'''Last Day of Classes<BR> '''NO LAB''' || December 12 || December 13|| December 14 ||
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| December 11 <br>'''Last Day of Classes<BR> '''NO LAB''' || December 12 || December 13|| December 14 ||
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|-
|}
|}
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<br>
<br>
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== '''BISC110/112 Assignments/ Weekly Lab Planner''' ==
 
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{| border=1px
 
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|'''Series'''
 
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|'''Lab #'''
 
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|<font color="red">'''LAB DATES'''</font color="red">
 
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|'''LAB DESCRIPTION'''
 
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|'''Assignment/Notes'''
 
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|--
 
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| colspan="6" |
 
-
|--
 
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| 1
 
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| 1
 
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| to<br>
 
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| Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br>
 
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| '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/S12:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br>
 
-
Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena''<BR> from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'',<BR> the non-selective phagocytosis seen in microstomes <BR>changes to a highly selective process in macrostomes.<BR> (2002), Journal of Experimental Biology 205, 2089-2097; <BR>
 
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'''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin,<BR> and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
 
-
|--
 
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| 1
 
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| 2
 
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|  to<br>
 
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| ''Tetrahymena'' Behavior: Phagocytosis; Observations; Data Analysis;<BR> Accuracy vs. Precision; Statistical Parameters;<BR> Data Presentation; Effective Figure Design and Legends<BR> using the Gronlien article as a model.
 
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| '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends from your ''Tetrahymena'' experiment:<BR> one or more microphotographs of Tetrahymena and a graph or table of data. Generate a figure of means +/- standard deviations and a caption for the theoretical data table at the end of the Assignments section in lab 2.
 
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|--
 
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| 1
 
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| 3
 
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|  to<br>
 
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| Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena'';<BR> Design your own experiment exploring<BR> inhibitors and ''Tetrahymena'' phagocytosis
 
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| '''Homework''': There are animated tutorials on dilution<BR> available at the following link:<BR> [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4; have it approved by your lab instructor;<br> Write a results section of a scientific report<BR> on your findings in the ''Tetrahymena'' toxicity experiment <BR>including a narrative portion and integrated figure(s) with legend(s);<br>
 
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Solve the dilution problems found at<BR> [[Media: Lab_3_110_Dilution_Problems.doc]] and hand them in<BR> by the beginning of Lab 4.
 
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|--
 
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| 1
 
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| 4
 
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|  to <br>
 
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| Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation;
 
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| '''Homework''': 5 minute oral presentation in PowerPoint™ of your data,<BR> including at least one figure with legend, of your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>'''* Gronlien, H.K., Berg, T., Lovlie, A.M.''' (2002).<BR> In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes<BR> to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097
 
-
|--
 
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| 1
 
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| 5
 
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| to <br>
 
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| Scientific Writing Workshop with Journal Article Discussion
 
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| '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/S12:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: <BR>[http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br>
 
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Have Lab notebooks ready to be turned in for inspection after Lab 6.
 
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<br> Read Lab 6 (first lab in Series 2, Genetics)
 
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<br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
 
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|--
 
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| 2
 
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| 6
 
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|  to<br>
 
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| Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR;
 
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| '''Homework''': Read the following background information on<BR> [http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf restriction enzymes] <BR>and on [http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf gel electrophoresis] <br>
 
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Read the following background information on ethical issues in genetic testing:<br>
 
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'''[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710700/?tool=pubmed Direct-to-Consumer Genetic Testing,'''<BR> Two Sides of the Coin] by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18. <BR>
 
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[http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf PUBLIC HEALTH: <BR>A Case Study of Personalized Medicine] by S. H. Katsanis, G. Javitt, and K. Hudson,<BR> published in ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)<BR>
 
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[http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br>
 
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Draw the expected migration locations of the Fnu4H1 digested PCR product<BR> obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br>
 
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Answer the questions found at:<BR>[[Media:Taster_DTC_questions110112_S12.doc‎]]<BR>to use as a template for your answers.'''
 
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|--
 
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| colspan="6" |
 
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|--
 
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| 2
 
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| 7
 
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| to <br>
 
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| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;<BR> Determine PTC Taster Genotype; Scientific Writing: <BR>Introduction & Discussion
 
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| '''Homework:
 
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Prepare draft figures/tables from the preliminary course data<BR> for the taster investigation paper and an skeleton outline<BR> of your introduction & discussion sections.<BR> (Refer to the handout on How to Prepare a Skeleton Outline in Sakai.)
 
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|--
 
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| 2 & 3
 
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| 8
 
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|to<br>
 
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| Data Analysis of Taster investigation; Science Writing Workshop:<BR> Effective Figure Design, Introduction, Discussion; Photosynthesis 1:<BR> Chlorophyll pigment investigation, Spectroscopy,<BR> The Beer-Lambert Law and Creating a Standard Curve
 
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| '''Homework''': Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.'''<BR> For help, see the [[BISC110/S12:Resources | Resources]] section of this wiki.<br>
 
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In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br>
 
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Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br>
 
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|--
 
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| 3
 
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| 9
 
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|to<br>
 
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| Photosynthesis 2: Hill Reaction
 
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| '''Homework''': Finalize your self-designed experiment<BR> testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction<BR> experiment that you did this week in Lab 9
 
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|--
 
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| 3
 
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| 10
 
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| to <br>
 
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| Photosynthesis 3: Investigation of variables affecting the Hill Reaction
 
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| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments (with the treatments on the same graph).<BR>See([[Appendix_E/S12 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots<BR> to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs)<BR> and plot those values against your variable (x-axis);<br>
 
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'''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11 (or on day indicated in Lab Calendar).'''<BR>  Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/S12:Guidelines for Science Writing | '''Guidelines for science writing''']]" information<BR> found in the [[BISC110/S12:Resources | Resources]] section of the wiki.
 
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|--
 
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| 3
 
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| 11
 
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|  to<br>
 
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| Science Writing Workshop<BR>Bring a draft of your Results & Discussion sections (5 points) <BR> (including figures/tables) of your photosynthesis paper
 
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| '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12'''<br>
 
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<br> Make sure Lab Notebook is ready to turn in for grading at the beginning of LAB 12
 
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|--
 
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| Skills Assessment
 
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| 12
 
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|  to <br>
 
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| '''Lab Practical'''; <br>Turn in Lab Notebook
 
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| End of lab
 
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|--
 
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|}
 
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<br>
 
=='''Lab Graded Assignments'''==
=='''Lab Graded Assignments'''==
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! 11
! 11
| 12 (or on day indicated in calendar)
| 12 (or on day indicated in calendar)
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| Full Scientific Paper: Factor Affecting Rate of Photosynthesis
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| Scientific Paper: Factor Affecting Rate of Photosynthesis
| 35
| 35
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|-
! 12  
! 12  
|12
|12
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|Lab Notebook Check (10) <BR>Lab Practical:Hands-on skills assessment (35)<br>
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|Lab Notebook Check (10) <BR>Lab Practical:Hands-on skills assessment (35) <br>
| 45  
| 45  
|-
|-

Current revision

Wellesley College

BISC110/112- Introduction to Cell Biology Lab- Fall 2012

   

BISC110/112 F12 Lab Calendar

Monday Tuesday Wednesday Thursday Friday
September 3
Labor Day
No Classes
September 4
Lab 1
Classes Start
September 5
Lab 1
September 6
Lab 1
September 7
Lab 1
September 10
Lab 1
September 11
Lab 2
September 12
Lab 2
September 13
Lab 2
September 14
Lab 2
September 17
Lab 2
September 18
Lab 3
September 19
Lab 3
September 20
Lab 3
September 21
Lab 3
September 24
Lab 3
September 25
Lab 4
September 26
Lab 4
September 27
Lab 4
September 28
Lab 4
October 1
Lab 4
October 2
Lab 5
October 3
Lab 5
October 4
Lab 5
October 5
Lab 5
October 8
FALL BREAK
NO CLASSES
October 9
FALL BREAK
NO CLASSES
October 10
NO LAB
October 11
NO LAB
October 12
NO LAB
October 15
LAB 5
October 16
Lab 6
October 17
Lab 6
October 18
Lab 6
October 19
Lab 6
October 22
Lab 6
October 23
Lab 7
October 24
Lab 7
October 25
Lab 7
October 26
Lab 7
October 29
Lab 7
October 30
Tanner Conference
No Classes
October 31
Lab 8
November 1
Lab 8
November 2
Lab 8
November 5
Lab 8
November 6
Lab 8
November 7
Lab 9
November 8
Lab 9
November 9
Lab 9
November 12
Lab 9
November 13
Lab 9
November 14
Lab 10
November 15
Lab 10
November 16
Lab 10
November 19
Lab 10
November 20
Lab 10
November 21
Thanksgiving Break
No Classes
November 22
Thanksgiving Break
No Classes
November 23
Thanksgiving Break
No Classes
November 26
Lab 11
November 27
Lab 11
November 28
Lab 11
November 29
Lab 11
November 30
Lab 11
December 3
Lab 12
December 4
Lab 12
December 5
Lab 12
December 6
Lab 12
December 7
Lab 12
December 10
NO LAB
December 11
Last Day of Classes
NO LAB
December 12 December 13 December 14


Schedule of Experiments

Section Lab # Activities
1 1-5 Boot Camp: Metric Measurement;
Using Basic Lab Equipment;
Making Solutions and Dilutions;
Microscopy; Scientific Investigation;
Designing & Executing Experiments;
Data Analysis & Display;
Statistical parameters; Scientific Writing
2 6-8 Genetics: DNA extraction, Visualization by agarose gel electrophoresis;
Genotype/phenotype via Taster SNP data collection and analysis;
Scientific Writing & Effective figure design
3 8-11 Photosynthesis: Spectrophotometry: Beer-Lambert Law, Linear regression analysis;
Hill Reaction: Self-designed investigation of factors
affecting photosystems
Lab Practical 12 Hands-on skills assessment:



Lab Graded Assignments

Lab Assigned Lab Due Assignment
description
Point Value
(Total 200)
1 2 Practice Problems 5
2 3 Fig. Design 5
3 4 Dilution Problems (5);
Fig.& Results (10)
15
4 5 Oral presentation: Results, original figure & legend;
Journal article discussion
10
5 6 Lab notebook check (ungraded);
Partial Scientific Paper(25)
25
6 7 SNP Taster Questions 5
7 8 Draft figures/tables & outline of Taster paper 5
8 9 Taster Scientific Paper (all but M&M) 35
9 10 M&M (5pt);
Finalize Self-Design
Experiment Hill Rxn.(ungraded)
5
10 11 Draft Introduction & Discussion and slides of figures/captions for Science Writing Workshop 5
11 12 (or on day indicated in calendar) Scientific Paper: Factor Affecting Rate of Photosynthesis 35
12 12 Lab Notebook Check (10)
Lab Practical:Hands-on skills assessment (35)
45
Other - Discretionary Points
Preparation & Participation
5


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