BISC110/F12:Series2

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Current revision (13:10, 8 June 2012) (view source)
(Series 2- To Taste Or Not To Taste)
 
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'''In this three week series of experiments you will learn:'''  <br>       
'''In this three week series of experiments you will learn:'''  <br>       
#About SNPs and how they are analyzed.
#About SNPs and how they are analyzed.
-
#How to isolate DNA from your own cells.
+
#How to isolate DNA from bacterial cells.
-
#How to use PCR to amplify a region of your PTC gene which contains a SNP.
+
#How to use PCR to amplify a region of the PTC gene which contains a SNP.
#How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
#How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
-
#How your own genotype and phenotype at the PTC locus correlate.
+
#How genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al.  2008.  Tasting phenylthiocarbamide (PTC):  a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
The following labs were adapted from Merritt, R. B., et. al.  2008.  Tasting phenylthiocarbamide (PTC):  a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
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Current revision

Wellesley College

BISC110/112- Introduction to Cell Biology Lab- Fall 2012

   

Links to Labs in Series 2

Lab 6 Taster SNP 1
Lab 7: Taster SNP 2
Lab 8: Taster Data Analysis & Photosynthesis 1

Series 2- To Taste Or Not To Taste

In this three week series of experiments you will learn:

  1. About SNPs and how they are analyzed.
  2. How to isolate DNA from bacterial cells.
  3. How to use PCR to amplify a region of the PTC gene which contains a SNP.
  4. How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
  5. How genotype and phenotype at the PTC locus correlate.


The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).



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