BISC110/F13:Series2

Links to Labs in Series 2

Lab 6 Taster SNP 1
Lab 7: Taster SNP 2
Lab 8: Spectrophotometry and Creating a Standard Curve

Series 2- Genetics and Disease

In this three week series of experiments you will learn:

1. About SNPs and how they are analyzed.
2. How to isolate DNA from bacterial cells.
3. How to use PCR to amplify a region of the PTC gene which contains a SNP.
4. How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
5. How genotype and phenotype at the PTC locus correlate.

The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).