BISC110/S10:Schedule

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| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Determine PTC Taster Genotype; Scientific Writing Workshop
| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Determine PTC Taster Genotype; Scientific Writing Workshop
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| '''Homework''':Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. For help, see the [[BISC110/S10:Resources |  Resources]] section of this wiki.
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| '''Homework''':Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. '''Due for all students Friday, April 2.'''For help, see the [[BISC110/S10:Resources |  Resources]] section of this wiki.
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| Due for all students<BR> Fri. 4/2
| Taster Scientific Paper (all but M&M)
| Taster Scientific Paper (all but M&M)
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Current revision

Wellesley College BISC110 Introduction to Cell Biology
Spring 2010


Contents

BISC110 Spring 2010 Lab Calendar

Monday Tuesday Wednesday Thursday Friday
January 25
January 26
Lab 1
January 27
Lab 1
January 28
Lab 1
January 29
Lab 1
February 1
Lab 1
February 2
Lab 2
February 3
Lab 2
February 4
Lab 2
February 5
Lab 2
February 8
Lab 2
February 9
Lab 3
February 10
Lab 3
February 11
Lab 3
February 12
Lab 3
February 15
Presidents' Day
No Lab
February 16
Monday Schedule
Lab 3
February 17
No Lab
February 18
No Lab
February 19
No Lab
February 22
Lab 4
February 23
Lab 4
February 24
Lab 4
February 25
Lab 4
February 26
Lab 4
March 1
Lab 5
March 2
Lab 5
March 3
Lab 5
March 4
Lab 5
March 5
Lab 5
March 8
Lab 6
March 9
Lab 6
March 10
Lab 6
March 11
Lab 6
March 12
Lab 6
March 15
Lab 7
March 16
Lab 7
March 17
Lab 7
March 18
Lab 7
March 19
Lab 7
March 22
Spring Break
March 23
Spring Break
March 24
Spring Break
March 25
Spring Break
March 26
Spring Break
March 29
Lab 8
March 30
Lab 8
March 31
Lab 8
April 1
Lab 8
April 2
Lab 8
April 5
Lab 9
April 6
Lab 9
April 7
Lab 9
April 8
Lab 9
April 9
Lab 9
April 12
Lab 10
April 13
Lab 10
April 14
Lab 10
April 15
Lab 10
April 16
Lab 10
April 19
Patriots' Day
No Lab
April 20
Lab 11
April 21
Lab 11
April 22
Monday Schedule
Lab 11
April 23
Lab 11
April 26
No Lab
April 27
No Lab
April 28
Rhulman
No Lab
April 29
Lab 11
April 30
Lab 12
May 3
Lab 12
May 4
Lab 12
May 5
Lab 12
May 6
Last Day of Classes
Lab 12

Schedule of Experiments

Section Lab # Activities
1 1-5 Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing
2 6-7 Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis
3 8-10 Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems
4 11 Gene Regulation/Enzymology: Invertase enzyme assay
Lab Practical 12 Hands-on skills assessment:


Assignments

BISC110 Assignments/ Weekly Lab Planner

Series Lab # LAB DATES__ LAB DESCRIPTION________ Assignment/Notes
1 1 Tues. Jan.26-
Mon. Feb 1
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis
Homework: Familiarize yourself with the wiki and particularly the information in the BISC110:Resources section of the Lab wiki;
Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_ProblemsS10.doc;

Read about the process of endocytosis in your textbook and familiarize yourself with phagocytosis in Tetrahymena from the following reference articles: Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097;
McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of Tetrahymena vorax., (2004), J Eukaryot. Microbiol., 51(2), 253-257.

1 2 Tues. Feb.2-
Mon. Feb.8
Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model. Homework: Study dilution making for Lab 3;
Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data.
1 3 Tues. Feb. 9-
Tues. Feb. 16
Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis Homework: There are animated tutorials on dilution making available at the following link: Dilution Help Tutorials;
Finalize your experimental design for Lab 4 and have it approved by your lab instructor;
Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends;

Work the dilution problems found at Media: Lab_3_S10_Dilution_Problems.doc and hand them in in Lab 4.

1 4 Mon. Feb. 22-
Fri. Feb. 26
Student Designed Experiments: Potential Inhibitors of Vacuole Formation; Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results.
Read assigned journal article for science writing discussion [Link to Wellesley College Library]
2 5 Mon. Mar.1-
Fri. Mar. 5
Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop; Homework: Partial lab report: Title Page, Results, Discussion and References sections. Guidelines at:BISC110/S10:Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources.

Have Lab notebooks ready to be turned in for inspection after Lab 6.
Read Lab 6 (first lab in Series 2, Genetics) Finding Sources
Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.

2 6 Mon. Mar. 8-
Fri. Mar.12
Genetics: Taster SNP 1-Test PTC Taster Phenotype; Isolate and digest DNA; PCR; Homework: Read the following background information on restriction enzymes found at:(http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf)

and on gel electrophoresis at:
(http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf); also Read the following background information on ethical issues in genetic testing: Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 and found at: (http://jmd.amjpathol.org/cgi/content/full/11/4/263); PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in Science, 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604] (in Policy Forum) found at: (http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf); AMA DTC testing policy recommendations at: (http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156);
Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel;
Answer the questions found at the following downloadable link:Media:Taster_DTC_questionsS10.doc‎ to use as a template for your answers.

2 7 Mon. Mar.15-
Fri. Mar. 19
Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Determine PTC Taster Genotype; Scientific Writing Workshop Homework:Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. Due for all students Friday, April 2.For help, see the Resources section of this wiki.
3 8 Mon. Mar. 29-
Fri. Apr. 2
Photosynthesis 1: Photosynthetic pigments Homework: Prepare a full results section for the photosynthetic pigment identification experiment;

In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).

3 9 Mon. April 5-
Fri. Apr. 9
Photosynthesis 2: Hill Reaction Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction;
Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9
3 10 Mon. Apr. 12-
Fri. Apr. 16
Photosynthesis 3: Investigation of variables affecting the Hill Reaction Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments with all or some of the treatments on the same graph.(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis);

A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis. Compare your results to other investigations published in the primary literature. Refer to the " Guidelines for science writing" information found in the Resources section of the wiki.

4 11 Tues. Apr. 20-
Thurs. Apr. 29
Enzymology Homework: Prepare for a Lab Practical Skills Assessment in lab 12.

Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions_110.doc
Make sure Lab Notebook is ready to turn in for grading

Skills Assessment 12 Fri Apr. 30-
Thurs. May 6
Lab Practical;
Turn in Lab Notebook
End of lab


Lab Graded Assignments

Lab Assigned Lab Due Assignment
description
Point Value
(Total 200)
1 2 Practice Problems 5
2 3 Fig. Design (ungraded) 0
3 4 Dilution Problems (5);
Fig.& Results (10)
15
4 5 Oral presentation: Results, original figure & legend;
Journal article discussion
10
5 6 Lab notebook check (ungraded);
Partial Scientific Paper(25)
25
6 7 SNP Taster Questions 5
7 Due for all students
Fri. 4/2
Taster Scientific Paper (all but M&M) 35
8 9 Photosyn. Pigments Results 10
9 10 M&M (5pt);
Finalize Self-Design
Experiment Hill Rxn.(ungraded)
5
10 11 Full Scientific Paper Hill Rxn. 35
11 12 Lab Notebook Check (10)
Concept Questions Enzymology/Gene Reg. (5)
15
12 12 Lab Practical:Hands-on skills assessment
35
Other - Discretionary Points
Preparation & Participation
5


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