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Wellesley College BISC110 Introduction to Cell Biology
Spring 2010


BISC110 Spring 2010 Lab Calendar

Monday Tuesday Wednesday Thursday Friday
January 25
January 26
Lab 1
January 27
Lab 1
January 28
Lab 1
January 29
Lab 1
February 1
Lab 1
February 2
Lab 2
February 3
Lab 2
February 4
Lab 2
February 5
Lab 2
February 8
Lab 2
February 9
Lab 3
February 10
Lab 3
February 11
Lab 3
February 12
Lab 3
February 15
Presidents' Day
No Lab
February 16
Monday Schedule
Lab 3
February 17
No Lab
February 18
No Lab
February 19
No Lab
February 22
Lab 4
February 23
Lab 4
February 24
Lab 4
February 25
Lab 4
February 26
Lab 4
March 1
Lab 5
March 2
Lab 5
March 3
Lab 5
March 4
Lab 5
March 5
Lab 5
March 8
Lab 6
March 9
Lab 6
March 10
Lab 6
March 11
Lab 6
March 12
Lab 6
March 15
Lab 7
March 16
Lab 7
March 17
Lab 7
March 18
Lab 7
March 19
Lab 7
March 22
Spring Break
March 23
Spring Break
March 24
Spring Break
March 25
Spring Break
March 26
Spring Break
March 29
Lab 8
March 30
Lab 8
March 31
Lab 8
April 1
Lab 8
April 2
Lab 8
April 5
Lab 9
April 6
Lab 9
April 7
Lab 9
April 8
Lab 9
April 9
Lab 9
April 12
Lab 10
April 13
Lab 10
April 14
Lab 10
April 15
Lab 10
April 16
Lab 10
April 19
Patriots' Day
No Lab
April 20
Lab 11
April 21
Lab 11
April 22
Monday Schedule
Lab 11
April 23
Lab 11
April 26
No Lab
April 27
No Lab
April 28
No Lab
April 29
Lab 11
April 30
Lab 12
May 3
Lab 12
May 4
Lab 12
May 5
Lab 12
May 6
Last Day of Classes
Lab 12

Schedule of Experiments

Section Lab # Activities
1 1-4 Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing
2 5-7 Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis; Case study, ethics
3 8-10 Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems
4 11 Gene Regulation/Enzymology: Invertase enzyme assay
Lab Practical 12 Hands-on skills assessment:


BISC110 Assignments/ Weekly Lab Planner

Series Lab # LAB DATES__ LAB DESCRIPTION________ Assignment/Notes
1 1 Mon. Jan.25-
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis; Making Dilutions; Determination of Non-toxic Concentration of Solutions
Homework: Familiarize yourself with the information in the BISC110/F09:Resources section of the Lab wiki;
Lab 1 Practice Problems downloadable here Media:Practice_Problems_Lab 1.doc

There are animated tutorials available at the following link: Dilution Help Tutorials

1 2 Mon. Feb.1-
Fri. Feb.5
Tetrahymena Behavior: Preparation of Ink Solutions; Observations & Hypothesis Generation; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Group Discussion & Hypothesis Generation Homework: Finalize your experimental design for Lab 3 and email a detailed protocol to your instructor well before the next lab;
Generate two figures with legends from your ink concentration experiment: one of one or more photographs of Tetrahymena and the other a graph of the data.
1 3 Mon. Feb. 8-
Fri. Feb. 12
Student Designed Experiments: Potential Inhibitors of Vacuole Formation Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your lab 3 experimental results.
Read assigned journal article: Chiesa R., Silva W., Renaud F., Pharmacological characteristics of an opoid receptor in the ciliate Tetrahymena. J. Euk. Microbiol. 406(6) 1993, 800-804.[Link to Wellesley College Library]
1 4 Mon. Feb. 22-
Fri. Feb. 26
Scientific Writing Workshop with Journal Article Discussion; Figure Presentation Workshop; Finding Sources Homework: Partial lab report: Title Page, Results, Discussion and References sections. Guidelines at:BISC110/F09:Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources.

Have Lab notebooks ready to be turned in for inspection after Lab 5.
Read Lab 5 (first lab in Series 2, Genetics)
Prepare a flow chart in your lab notebook of all Lab 5 experimental procedures.

2 5 Mon. Mar.1-
Fri. Mar. 5
Genetics: Taster SNP 1-Isolate and digest DNA; PCR; Start case study Homework: Read the following background information on restriction enzymes found at:(

and on gel electrophoresis at:
(; also Read the following background information for a case study on ethical issues in genetic testing: Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 and found at: (; PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in Science, 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604] (in Policy Forum) found at: (; AMA DTC testing policy recommendations at: (; Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel; Answer the questions found at the following downloadable link:Media:Media-Homework110Lab5.doc‎ to use as a template for your answers.

2 6 Mon. Mar. 8-
Fri. Mar.12
Genetics: Taster SNP 2; Prepare and perform agarose gel electrophoresis Homework: Prepare a formal figure of the gel picture you obtained today, complete with title, legend and labels. For help, see the Resources section of this wiki: " Guidelines for science writing";

Prepare for your lab group Oral Presentation on a DTC company. For help, see the Resources section of this wiki: Guidelines for Oral Presentations.

2 7 Mon. Mar.15-
Fri. Mar. 19
Genetics: Taster SNP 3: ; Oral Presentations & Scientific Writing Workshop Homework:Writing a paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. For help, see the Resources section of this wiki.
3 8 Mon. Mar. 29-
Fri. Apr. 2
Photosynthesis 1: Photosynthetic pigments Homework: Media:Assignment for Lab 8.doc Download Questions (Answers to be turned in next lab) through this link;

Label your printed spectra and chromatogram, and write figure legends for them;
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).

3 9 Mon. April 5-
Fri. Apr. 9
Photosynthesis 2: Hill Reaction Homework: E-mail your self-designed experiment testing a variable affecting the Hill Reaction to your lab instructor (cc your partner) by your instructor's deadline for first submission;
Modify the proposal after receiving your instructor's suggestions and resubmit it by your instructor's deadline;
Proposals must include:– your hypothesis, controls and expected results as well as your protocol (with sufficient detail, e.g., how to set up your tubes, etc).
3 10 Mon. Apr. 12-
Fri. Apr. 16
Photosynthesis 3: Investigation of variables affecting the Hill Reaction Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (you can plot several or all data sets on one graph). Plot all data on the same graph as regression lines for ease of comparison (Appendix E). Include the regression equations. The slopes represent the rates of the Hill reactions. Do not include points that have leveled off. Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis).

A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate and the coupling of electron transport with the photophosphorylation of ADP and/or the role of the H+ gradient in these processes. Compare your results to other investigations published in the primary literature. Refer to the " Guidelines for science writing" information found in the Resources section of the wiki.

4 11 Tues. Apr. 20-
Thurs. Apr. 29
Enzymology Homework: Prepare for a Lab Practical Skills Assessment in lab 12.

Answer the following questions (10 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions.doc
Make sure Lab Notebook is ready to turn in for grading

Skills Assessment 12 Fri Apr. 30-
Thurs. May 6
Lab Practical;
Turn in Lab Notebook
End of lab

Lab Graded Assignments

Lab Assigned Lab Due Assignment
Point Value
(Total 200)
1 2 Practice Problems 5
2 3 Fig. Design 5
3 4 Dilution Problems (5);
Fig.& Results (10)
4 5 Oral presentation: Results, original figure & legend;
Journal article discussion
5 6 Lab notebook check (ungraded);
Partial Scientific Paper(25)
6 7 SNP Taster Questionsbr> 10
7 8 Taster Scientific Paper (all but M&M) 35
8 9 Photosyn. Pigments Results 10
9 10 M&M (5pt);
Finalize Self-Design
Experiment Hill Rxn.(ungraded)
10 11 Full Scientific Paper Hill Rxn. 35
11 12 Lab Notebook Check (10)
Concept Questions Enzymology/Gene Reg. (10)
12 12 Lab Practical:Hands-on skills assessment
Other - Discretionary Points
Preparation & Participation

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