BISC110/S10:Schedule
BISC110 Spring 2010 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday | |
---|---|---|---|---|---|
January 25 |
January 26 Lab 1 |
January 27 Lab 1 |
January 28 Lab 1 |
January 29 Lab 1 |
|
February 1 Lab 1 |
February 2 Lab 2 |
February 3 Lab 2 |
February 4 Lab 2 |
February 5 Lab 2 |
|
February 8 Lab 2 |
February 9 Lab 3 |
February 10 Lab 3 |
February 11 Lab 3 |
February 12 Lab 3 |
|
February 15 Presidents' Day No Lab |
February 16 Monday Schedule Lab 3 |
February 17 No Lab |
February 18 No Lab |
February 19 No Lab |
|
February 22 Lab 4 |
February 23 Lab 4 |
February 24 Lab 4 |
February 25 Lab 4 |
February 26 Lab 4 |
|
March 1 Lab 5 |
March 2 Lab 5 |
March 3 Lab 5 |
March 4 Lab 5 |
March 5 Lab 5 |
|
March 8 Lab 6 |
March 9 Lab 6 |
March 10 Lab 6 |
March 11 Lab 6 |
March 12 Lab 6 |
|
March 15 Lab 7 |
March 16 Lab 7 |
March 17 Lab 7 |
March 18 Lab 7 |
March 19 Lab 7 |
|
March 22 Spring Break |
March 23 Spring Break |
March 24 Spring Break |
March 25 Spring Break |
March 26 Spring Break |
|
March 29 Lab 8 |
March 30 Lab 8 |
March 31 Lab 8 |
April 1 Lab 8 |
April 2 Lab 8 |
|
April 5 Lab 9 |
April 6 Lab 9 |
April 7 Lab 9 |
April 8 Lab 9 |
April 9 Lab 9 |
|
April 12 Lab 10 |
April 13 Lab 10 |
April 14 Lab 10 |
April 15 Lab 10 |
April 16 Lab 10 |
|
April 19 Patriots' Day No Lab |
April 20 Lab 11 |
April 21 Lab 11 |
April 22 Monday Schedule Lab 11 |
April 23 Lab 11 |
|
April 26 No Lab |
April 27 No Lab |
April 28 Rhulman No Lab |
April 29 Lab 11 |
April 30 Lab 12 |
|
May 3 Lab 12 |
May 4 Lab 12 |
May 5 Lab 12 |
May 6 Last Day of Classes Lab 12 |
Schedule of Experiments
Section | Lab # | Activities |
---|---|---|
1 | 1-5 | Boot Camp: Metric Measurement; Using Basic Lab Equipment; Making Solutions and Dilutions; Microscopy; Scientific Investigation; Designing & Executing Experiments; Data Analysis & Display; Statistical parameters; Scientific Writing |
2 | 6-7 | Genetics: DNA extraction, Visualization by agarose gel electrophoresis; Genotype/phenotype via Taster SNP data collection and analysis; Case study, ethics |
3 | 8-10 | Photosynthesis: Extraction of photosynthetic pigments; Spectrophotometry: Beer-Lambert Law, Linear regression analysis; Hill Reaction: Self-designed investigation of factors affecting photosystems |
4 | 11 | Gene Regulation/Enzymology: Invertase enzyme assay |
Lab Practical | 12 | Hands-on skills assessment: |
Assignments
BISC110 Assignments/ Weekly Lab Planner
Series | Lab # | LAB DATES__ | LAB DESCRIPTION________ | Assignment/Notes | ||
1 | 1 | Mon. Jan.25- Jan.29 |
Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis; Making Dilutions; Determination of Non-toxic Concentration of Solutions |
Homework: Familiarize yourself with the information in the BISC110/F09:Resources section of the Lab wiki; Lab 1 Practice Problems downloadable here Media:Practice_Problems_Lab 1.doc There are animated tutorials available at the following link: Dilution Help Tutorials | ||
1 | 2 | Mon. Feb.1- Fri. Feb.5 |
Tetrahymena Behavior: Preparation of Ink Solutions; Observations & Hypothesis Generation; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Group Discussion & Hypothesis Generation | Homework: Finalize your experimental design for Lab 3 and email a detailed protocol to your instructor well before the next lab; Generate two figures with legends from your ink concentration experiment: one of one or more photographs of Tetrahymena and the other a graph of the data. | ||
1 | 3 | Mon. Feb. 8- Fri. Feb. 12 |
Student Designed Experiments: Potential Inhibitors of Vacuole Formation | Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your lab 3 experimental results. Read assigned journal article: Chiesa R., Silva W., Renaud F., Pharmacological characteristics of an opoid receptor in the ciliate Tetrahymena. J. Euk. Microbiol. 406(6) 1993, 800-804.[Link to Wellesley College Library] | ||
1 | 4 | Mon. Feb. 22- Fri. Feb. 26 |
Scientific Writing Workshop with Journal Article Discussion; Figure Presentation Workshop; Finding Sources | Homework: Partial lab report: Title Page, Results, Discussion and References sections. Guidelines at:BISC110/F09:Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources. Have Lab notebooks ready to be turned in for inspection after Lab 5.
| ||
2 | 5 | Mon. Mar.1- Fri. Mar. 5 |
Genetics: Taster SNP 1-Isolate and digest DNA; PCR; Start case study | Homework: Read the following background information on restriction enzymes found at:(http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf)
and on gel electrophoresis at: | ||
2 | 6 | Mon. Mar. 8- Fri. Mar.12 |
Genetics: Taster SNP 2; Prepare and perform agarose gel electrophoresis | Homework: Prepare a formal figure of the gel picture you obtained today, complete with title, legend and labels. For help, see the Resources section of this wiki: " Guidelines for science writing"; Prepare for your lab group Oral Presentation on a DTC company. For help, see the Resources section of this wiki: Guidelines for Oral Presentations. | ||
2 | 7 | Mon. Mar.15- Fri. Mar. 19 |
Genetics: Taster SNP 3: ; Oral Presentations & Scientific Writing Workshop | Homework:Writing a paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. For help, see the Resources section of this wiki. | ||
3 | 8 | Mon. Mar. 29- Fri. Apr. 2 |
Photosynthesis 1: Photosynthetic pigments | Homework: Media:Assignment for Lab 8.doc Download Questions (Answers to be turned in next lab) through this link; Label your printed spectra and chromatogram, and write figure legends for them; | ||
3 | 9 | Mon. April 5- Fri. Apr. 9 |
Photosynthesis 2: Hill Reaction | Homework: E-mail your self-designed experiment testing a variable affecting the Hill Reaction to your lab instructor (cc your partner) by your instructor's deadline for first submission; Modify the proposal after receiving your instructor's suggestions and resubmit it by your instructor's deadline; Proposals must include:– your hypothesis, controls and expected results as well as your protocol (with sufficient detail, e.g., how to set up your tubes, etc). | ||
3 | 10 | Mon. Apr. 12- Fri. Apr. 16 |
Photosynthesis 3: Investigation of variables affecting the Hill Reaction | Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (you can plot several or all data sets on one graph). Plot all data on the same graph as regression lines for ease of comparison (Appendix E). Include the regression equations. The slopes represent the rates of the Hill reactions. Do not include points that have leveled off. Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis). A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate and the coupling of electron transport with the photophosphorylation of ADP and/or the role of the H+ gradient in these processes. Compare your results to other investigations published in the primary literature. Refer to the " Guidelines for science writing" information found in the Resources section of the wiki. | ||
4 | 11 | Tues. Apr. 20- Thurs. Apr. 29 |
Enzymology | Homework: Prepare for a Lab Practical Skills Assessment in lab 12. Answer the following questions (10 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions.doc
| ||
Skills Assessment | 12 | Fri Apr. 30- Thurs. May 6 |
Lab Practical; Turn in Lab Notebook |
End of lab |
Lab Graded Assignments
Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
---|---|---|---|
1 | 2 | Practice Problems | 5 |
2 | 3 | Fig. Design (ungraded) | 0 |
3 | 4 | Dilution Problems (5); Fig.& Results (10) |
15 |
4 | 5 | Oral presentation: Results, original figure & legend; Journal article discussion |
10 |
5 | 6 | Lab notebook check (ungraded); Partial Scientific Paper(25) |
25 |
6 | 7 | SNP Taster Questions | 5 |
7 | 8 | Taster Scientific Paper (all but M&M) | 35 |
8 | 9 | Photosyn. Pigments Results | 10 |
9 | 10 | M&M (5pt); Finalize Self-Design Experiment Hill Rxn.(ungraded) |
5 |
10 | 11 | Full Scientific Paper Hill Rxn. | 35 |
11 | 12 | Lab Notebook Check (10) Concept Questions Enzymology/Gene Reg. (5) |
15 |
12 | 12 | Lab Practical:Hands-on skills assessment |
35 |
Other | - | Discretionary Points Preparation & Participation |
5 |