|| Tues. Jan.26-|
Mon. Feb 1
| Practice metric conversions; Practice pipetting; Making Solutions; Microscopy; Examination of Tetrahymena pyriformis|
| Homework: Familiarize yourself with the wiki and particularly the information in the BISC110:Resources section of the Lab wiki;|
Lab 1 Practice Problems downloadable here Media:Lab_1_Practice_Problems.doc;
Read about the process of endocytosis in your textbook and familiarize yourself with phagocytosis in Tetrahymena from the following reference articles: Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097;
McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of Tetrahymena vorax., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
|| Tues. Feb.2- |
| Tetrahymena Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model.
|| Homework: Study dilution making for Lab 3; |
Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data.
|| Tues. Feb. 9-|
Tues. Feb. 16
| Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis
|| Homework: There are animated tutorials on dilution making available at the following link: Dilution Help Tutorials; |
Finalize your experimental design for Lab 4 and have it approved by your lab instructor;
Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends;
Work the dilution problems found at Media: Lab_3_Dilution_Problems.doc and hand them in in Lab 4.
|| Mon. Feb. 22- |
Fri. Feb. 26
| Student Designed Experiments: Potential Inhibitors of Vacuole Formation;
|| Homework: 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. |
Read assigned journal article for science writing discussion [Link to Wellesley College Library]
|| Mon. Mar.1-|
Fri. Mar. 5
| Scientific Writing Workshop with Journal Article Discussion; Science Writing Workshop;
|| Homework: Partial lab report: Title Page, Results, Discussion and References sections. Guidelines at:BISC110/F09:Resources; Science Writing peer-tutor appointment scheduling at: Wellesley College Writing Program Resources.|
Have Lab notebooks ready to be turned in for inspection after Lab 6.
Read Lab 6 (first lab in Series 2, Genetics) Finding Sources
Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
|| Mon. Mar. 8- |
| Genetics: Taster SNP 1-Isolate and digest DNA; PCR;
|| Homework: Read the following background information on restriction enzymes found at:(http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf)
and on gel electrophoresis at:
Read the following background information on ethical issues in genetic testing:
Direct-to-Consumer Genetic Testing, Two Sides of the Coin by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18 and found at: (http://jmd.amjpathol.org/cgi/content/full/11/4/263);
PUBLIC HEALTH: A Case Study of Personalized Medicine by S. H. Katsanis, G. Javitt, and K. Hudson,published in
Science, 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604] (in Policy Forum) found
AMA DTC testing policy recommendations at: (http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156);
Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel;
Answer the questions found at the following downloadable link:Media:Taster_DTC_questions.doc to use as a template for your answers.
|| Mon. Mar.15- |
Fri. Mar. 19
| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis ; Scientific Writing Workshop
|| Homework:Writing a paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. For help, see the Resources section of this wiki.
|| Mon. Mar. 29- |
Fri. Apr. 2
| Photosynthesis 1: Photosynthetic pigments
|| Homework: Media:Assignment for Lab 8.doc Download Questions (Answers to be turned in next lab) through this link;|
Label your printed spectra and chromatogram, and write figure legends for them;
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).
|| Mon. April 5- |
Fri. Apr. 9
| Photosynthesis 2: Hill Reaction
|| Homework: E-mail your self-designed experiment testing a variable affecting the Hill Reaction to your lab instructor (cc your partner) by your instructor's deadline for first submission;|
Modify the proposal after receiving your instructor's suggestions and resubmit it by your instructor's deadline;
Proposals must include:– your hypothesis, controls and expected results as well as your protocol (with sufficient detail, e.g., how to set up your tubes, etc).
|| Mon. Apr. 12- |
Fri. Apr. 16
| Photosynthesis 3: Investigation of variables affecting the Hill Reaction
|| Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (you can plot several or all data sets on one graph). Plot all data on the same graph as regression lines for ease of comparison (Appendix E). Include the regression equations. The slopes represent the rates of the Hill reactions. Do not include points that have leveled off. Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis).|
A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate and the coupling of electron transport with the photophosphorylation of ADP and/or the role of the H+ gradient in these processes. Compare your results to other investigations published in the primary literature. Refer to the " Guidelines for science writing" information found in the Resources section of the wiki.
|| Tues. Apr. 20-|
Thurs. Apr. 29
|| Homework: Prepare for a Lab Practical Skills Assessment in lab 12.|
Answer the following questions (10 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions.doc
Make sure Lab Notebook is ready to turn in for grading
| Skills Assessment
|| Fri Apr. 30-|
Thurs. May 6
| Lab Practical; |
Turn in Lab Notebook
| End of lab
| Lab Assigned || Lab Due || Assignment|
| Point Value |
|| Practice Problems
|| Fig. Design (ungraded)
|| Dilution Problems (5);|
Fig.& Results (10)
|| Oral presentation: Results, original figure & legend;|
Journal article discussion
|| Lab notebook check (ungraded);|
Partial Scientific Paper(25)
|| SNP Taster Questions
|| Taster Scientific Paper (all but M&M)
|| Photosyn. Pigments Results
|| M&M (5pt);|
Experiment Hill Rxn.(ungraded)
|| Full Scientific Paper Hill Rxn.
|| Lab Notebook Check (10) |
Concept Questions Enzymology/Gene Reg. (5)
||Lab Practical:Hands-on skills assessment|
|| Discretionary Points |
Preparation & Participation