BISC110/S11:Series2: Difference between revisions
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=='''Series 2- To Taste Or Not To Taste'''== | |||
'''In this three week series of experiments you will learn:''' <br> | |||
#About SNPs and how they are analyzed. | |||
#How to isolate DNA from your own cells. | |||
#How to use PCR to amplify a region of your PTC gene which contains a SNP. | |||
#How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants. | |||
#How your own genotype and phenotype at the PTC locus correlate. | |||
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html). | |||
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[[BISC110/S11: Lab 6 Taster SNP1 | Lab 6 Taster SNP 1]]<br> | |||
[[BISC110/S11: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | |||
<br> | |||
<br> | |||
Latest revision as of 07:27, 7 December 2010
Series 2- To Taste Or Not To Taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from your own cells.
- How to use PCR to amplify a region of your PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How your own genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
