BISC110/S11:Series2
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| + | =='''Series 2- To Taste Or Not To Taste'''== | ||
| + | '''In this three week series of experiments you will learn:''' <br> | ||
| + | #About SNPs and how they are analyzed. | ||
| + | #How to isolate DNA from your own cells. | ||
| + | #How to use PCR to amplify a region of your PTC gene which contains a SNP. | ||
| + | #How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants. | ||
| + | #How your own genotype and phenotype at the PTC locus correlate. | ||
| + | |||
| + | |||
| + | The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html). | ||
| + | <br><br> | ||
| + | |||
| + | [[BISC110/S11: Lab 6 Taster SNP1 | Lab 6 Taster SNP 1]]<br> | ||
| + | [[BISC110/S11: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | ||
| + | <br> | ||
| + | <br> | ||
Current revision
Series 2- To Taste Or Not To Taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from your own cells.
- How to use PCR to amplify a region of your PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How your own genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
Lab 6 Taster SNP 1
Lab 7: Taster SNP 2
