BISC110/S13:Series2: Difference between revisions
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==Links to Labs in Series 2== | ==Links to Labs in Series 2== | ||
[[BISC110/ | [[BISC110/F12: Lab 6 Taster SNP1 | Lab 6 Taster SNP 1]]<br> | ||
[[BISC110/S13: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | [[BISC110/S13: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | ||
[[BISC110/S13: Series 3 Lab 8| Lab 8: Spectrophotometry and Creating a Standard Curve]]<br> | [[BISC110/S13: Series 3 Lab 8| Lab 8: Spectrophotometry and Creating a Standard Curve]]<br> | ||
Revision as of 08:16, 14 January 2013
Links to Labs in Series 2
Lab 6 Taster SNP 1
Lab 7: Taster SNP 2
Lab 8: Spectrophotometry and Creating a Standard Curve
Series 2- To Taste Or Not To Taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from bacterial cells.
- How to use PCR to amplify a region of the PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
