BISC110/S13:Series2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| (4 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Template:BISC110/S13}} | {{Template:BISC110/S13}} | ||
<div style="padding: 10px; width: 725px; border: 5px solid # | <div style="padding: 10px; width: 725px; border: 5px solid #00cc66;"> | ||
==Links to Labs in Series 2== | ==Links to Labs in Series 2== | ||
[[BISC110/ | [[BISC110/S13: Lab 6 Taster SNP1 | Lab 6 Taster SNP 1]]<br> | ||
[[BISC110/ | [[BISC110/S13: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | ||
[[BISC110/ | [[BISC110/S13: Series 3 Lab 8| Lab 8: Spectrophotometry and Creating a Standard Curve]]<br> | ||
<br> | <br> | ||
=='''Series 2- | =='''Series 2- Genetics and Disease'''== | ||
'''In this three week series of experiments you will learn:''' <br> | '''In this three week series of experiments you will learn:''' <br> | ||
#About SNPs and how they are analyzed. | #About SNPs and how they are analyzed. | ||
Latest revision as of 11:15, 5 March 2013
Links to Labs in Series 2
Lab 6 Taster SNP 1
Lab 7: Taster SNP 2
Lab 8: Spectrophotometry and Creating a Standard Curve
Series 2- Genetics and Disease
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from bacterial cells.
- How to use PCR to amplify a region of the PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
