Series 1 Lab 4 Variables Affecting Phagocytosis
Adapted from Bozzone, M.D., and D.A. Martin 2000. An experimenal system to study phagocytosis. Pages 405-415, in Tested studies for laboratory teaching, Volume 21 (S.J. Karcher, Editor). Proceedings of the 21st Workshop/Conference of the Association for Biology Laboratory Education (ABLE).
You will have the opportunity to carry out an experiment that you and your partner design. Each group of four students will focus on one variable.
- Place used microcentrifuge tubes that DO NOT contain gluteraldehyde in autoclave bags taped above your bench. Place used tips that do not contain gluteraldehyde in the regular trash container.
- Any microcentrifuge tubes containing gluteraldehyde should be disposed of in the hood.
- Put all used microscope slides and cover slips that DO NOT contain gluteraldehyde in the glass disposal box.
- Slides containing gluteraldehyde can be disposed of in the waste container located in the hood.
- Rotate the 4x objective lens into place.
- The binocular head must be rotated into the storage position, to protect the ocular lenses from damage. Loosen the setscrew on the right, rotate the head 180°, then tighten the screw. Turn off the microscope light. Return the microscope to the cabinet under your bench with its plastic cover on.
- Put all used microscope slides and cover slips in the glass disposal box.
- Anything that was exposed to gluteraldehyde but NOT exposed to glycine should have been disposed of in the hood.
Preparation for Science Writing Workshop in Lab 5
- (5 points) In lab 5, you and your partner or group will give a 5 minute oral presentation showing your data and explaining how it addresses your experimental question and topic. Please prepare a few PowerPoint slides that include at least one figure and figure legend(s) summarizing the main points from your data. Although figure legends are not typically included in PowerPoint presentations (since this is the part that you explain verbally), you will include the legend this time so we can discuss effective figure design. Be sure and upload your presentation to the folder your instructor designates in the lab Sakai site before Lab 5 begins so that you can access it on the computer in the presentation classroom. We will not be meeting in the lab for your presentations. Your instructor will let you know where to meet.
- Read the Gronlien, et al. (2002) journal article carefully. Now that you have done your own investigation of phagocytosis in Tetrahymena and created your own figures from your data, you are better equipped to think about and discuss how scientific findings are presented. We will have a Science Writing Workshop after the presentations in Lab 5. Pay careful attention to the format of the different sections of the Gronlien paper. The main purpose of our workshop is to help you understand the structure of scientific writing so that you will be prepared to write a partial scientific research paper of your own.
- Take the Lab 5 pre-lab quiz.