BISC110:Series2: Difference between revisions
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[[BISC110/S10: Lab 6 Taster SNP1 | Lab 6 Taster SNP 1]]<br> | [[BISC110/S10: Lab 6 Taster SNP1 | Lab 6 Taster SNP 1]]<br> | ||
[[BISC110/S10: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | [[BISC110/S10: Lab 7 Taster SNP2 | Lab 7: Taster SNP 2]]<br> | ||
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Latest revision as of 12:19, 19 December 2009
Series 2- To Taste Or Not To Taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from your own cells.
- How to use PCR to amplify a region of your PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How your own genotype and phenotype at the PTC locus correlate.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
