BISC209/F13: Lab9

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(Complete Testing for Antibiotic Producers Among Your Cultured Isolates)
(Complete Testing for Antibiotic Producers Among Your Cultured Isolates)
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'''Week 3 <BR>'''
'''Week 3 <BR>'''
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<UL><LI>
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Examine the plates you inoculated with control bacterial cultures last week and look for evidence of a zone of inhibition of growth of "test" organisms near the antibiotic producer's midline streak  <LI>
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Examine the plates you inoculated with known bacterial cultures last week and look for evidence of a zone of inhibition of growth near the antibiotic producer's midline streak  <LI>
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Draw the results and evaluate whether or not there was evidence that an antibiotic was produced by the organism and, if so, which of the bacteria tested were sensitive to it and to what degree. If you found no inhibition of growth, does that mean that your potential antibiotic producer does not secrete an any antimicrobial compounds? Why or why not? </LI></UL>
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Draw the results and evaluate whether or not there was evidence that an antibiotic was produced by your isolate and, if so, which of the known bacteria were sensitive to it and to what degree. If you found no inhibition of growth, does that mean that your isolate does not secrete an any antimicrobial compounds? Why or why not? </LI></UL>
[[Image:antibiotic.jpg]].
[[Image:antibiotic.jpg]].

Revision as of 07:59, 19 November 2013

Wellesley College- BISC209 Microbiology- Fall 2013

Contents

LAB 9 Analyzing Your DNA Sequencing data and Finishing the Project

You will have a workshop to evaluate and analyze your 16S rRNA gene sequencing data

Complete Testing for Antibiotic Producers Among Your Cultured Isolates

Week 3

  • Examine the plates you inoculated with known bacterial cultures last week and look for evidence of a zone of inhibition of growth near the antibiotic producer's midline streak
  • Draw the results and evaluate whether or not there was evidence that an antibiotic was produced by your isolate and, if so, which of the known bacteria were sensitive to it and to what degree. If you found no inhibition of growth, does that mean that your isolate does not secrete an any antimicrobial compounds? Why or why not?

Image:antibiotic.jpg.

Finishing the project

After you have recorded all your isolate test results on a lab Google Doc, please discard all cultures. You will receive 5 "clean-up points" if we do not find any evidence of your cultures or tests you will receive 5 points toward your lab grade. If we have to discard and clean-up later after you, you will not receive any of those points. The date that clean up must be completed will be announced by your instructor.

Assignment

Study for your Lab Practical (45points)
Information and Tips can be found at : Lab Practical.
Your final paper is due on Tues. Dec. 10 by 4pm. (the last day of classes) for all students. You have all the data you need to start working on it. Instructions can be found at: Assignment: Final Paper

To Do:
Discard and clean up any remaining test materials and old cultures of your isolates. Note that there are 5 bonus points awarded for perfect clean-up. Your instructor will explain the criteria for obtaining these points.

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10


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