BISC209/S10:Schedule: Difference between revisions
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== ''' Assignments ''' == | == ''' Assignments ''' == | ||
BISC110 Assignments/ Weekly Lab Planner | |||
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| 2 | | 2 | ||
| Tues. 2/2-<br>Wed. 2/3 | | Tues. 2/2-<br>Wed. 2/3 | ||
| | | environmental sampling (steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc. | ||
environmental sampling (steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc. | |||
NOTE: MEDIA -use Janssen media-dilute broth pg 2392 (Improved culturability of soil bacteria dn isolation: to enrich for soil groups. | NOTE: MEDIA -use Janssen media-dilute broth pg 2392 (Improved culturability of soil bacteria dn isolation: to enrich for soil groups. | ||
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Discuss habitats and literature searches | Discuss habitats and literature searches | ||
Freeze DNA | Freeze DNA | ||
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|'''Homework''' | |'''Homework''' |
Revision as of 11:17, 16 December 2009
BISC209 S10 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday |
---|---|---|---|---|
Jan.25 | Jan. 26 Lab 1 |
Jan. 27 Lab 1 |
Jan. 28 | Jan. 29 |
Feb. 1 | Feb. 2 Lab 2 |
Feb. 3 Lab 2 |
Feb. 4 | Feb. 5 |
Feb. 8 | Feb. 9 Lab 3 |
Feb. 10 Lab 3 |
Feb. 11 | Feb. 12 |
Feb. 15 President's Day |
Feb. 16 Monday schedule NO LAB |
Feb. 17 NO LAB |
Feb. 18 | Feb. 19 |
Feb. 22 | Feb. 23 Lab 4 |
Feb. 24 Lab 4 |
Feb. 25 | Feb. 26 |
Mar. 1 | Mar. 2 Lab 5 |
Mar. 3 Lab 5 |
Mar. 4 | Mar. 5 |
Mar. 8 | Mar. 9 Lab 6 |
Mar. 10 Lab 6 |
Mar. 11 | Mar. 12 |
Mar. 15 | Mar. 16 Lab 7 |
Mar. 17 Lab 7 |
Mar. 18 | Mar. 19 |
Mar. 22 Spring Break |
Mar. 23 Spring Break |
Mar. 24 Spring Break |
Mar. 25 Spring Break |
Mar. 26 Spring Break |
Mar. 29 | Mar. 30 Lab 8 |
Mar. 31 Lab 8 |
Apr. 1 | Apr. 2 |
Apr. 5 | Apr. 6 Lab 9 |
Apr. 6 Lab 9 |
Apr. 7 | Apr. 8 |
Apr. 12 | Apr. 13 Lab 10 |
Apr. 14 Lab 10 |
Apr. 15 | Apr. 16 |
Apr. 19 Patriots' Day NO LAB |
Apr. 20 Lab 11 |
Apr. 21 Lab 11 |
Apr. 22 Monday Schedule |
Apr. 23 |
Apr. 26 | Apr. 27 NO Lab |
Apr. 28 Rhulman NO Lab |
Apr. 29 | April 30 |
May 3 | May 4 Lab 12 |
May 5 Lab 12 |
May 6 Last day of classes |
April 30 |
Assignments
BISC110 Assignments/ Weekly Lab Planner
Lab # | LAB DATES__ | IN LAB WORK________ | OUTSIDE OF LAB WORK________ | Assignment/Notes | ||
1 | Tues. 1/26- Wed. 1/27 |
intro to micro lab lab safety: BSL 2-concepts etc
|
Homework: | |||
2 | Tues. 2/2- Wed. 2/3 |
environmental sampling (steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc.
NOTE: MEDIA -use Janssen media-dilute broth pg 2392 (Improved culturability of soil bacteria dn isolation: to enrich for soil groups. ENVIRONMENTAL SAMPLING: (steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc. Sample dilution for single colonies techniques also? (Wagner-NABT paper) Plan on 4 students per site and each pair sampling from the site (so 6 samplesper lab)- most protocols the samples go into broth (see seifert) ON own- isolation streak a new plate. Each individual. DNA isolation- in pairs Isolating genomic DNA from soil samples (mo bio lab protocol): using mobio ultraclean soil dna purification kit Nano dropper procedure (using machine-on screen read out) dilute dna to proper concentration and freeze for DNA library process (PCR etc see MBL flow chart and diagram lab 12 OR for rDNA library steps) Discuss habitats and literature searches Freeze DNA |
Homework | |||
3 | Tues. 2/9- Wed. 2/10 |
DNA diversity: complete prep for pyrosequencing? OR rDNA library steps: (Use soil DNA from lab 2)
DNA library: Clean and PCR Amplify for rDNA using Irene's universal primers (Irene's notes)- 3 hours
techniques: colony morphology, number (see mIcrobial safari= wagner) and problem solve to "discover" 2 isolation methods (serial dilution and pour plates vs streaking for isolation) Isolation to pure colony step: #1: How will they pick what/where on plate to isolate? (secondary plates) Each student picks different 4-6 colonies and restreaks on same media (4-6 different plates). Incubate room temp.grow 24 hours, move to CR.
Control stocks for: gram pos, gram neg and capsule, acid fast and endospore Simple stain Gram stain What do you learn from this? |
Homework: | |||
4 | Tues. 2/23- Wed. 2/24 |
Run gel on PCR from last time
Clone 16s rDNA using TOPO dna cloning kit and MBL lab 12 to complete transformation incubate overnight in plates (see mbl protocol)
ID from secondary cultures (Steubing protocol): on real unknown Techniques: repeat all stains from week before: at least - simple stain (3 pure colonies) gram stain etc (and be sure all stains from week 3 are performed).
Introduce: stains and microscopy: on control stock orgs. -negative stain (for capsules) -acid fast -endospore |
Homework: | |||
5 | Tues. 3/2- Wed. 3/3 |
Select 2 clones and put in 96 well plates (how? protocol ends here) and send them out? where? How long?
UNKNOWN - using stock (1-2 or 3 made) make 2 more stock slants Techniques: all stains: on unknowns (2-3) capsule endospore acid fast etc |
Homework: | |||
6 | Tues.3/9. - Wed. 3/10 |
Unknown:
Physical: Sterbing lab (9-15) soft agar deeps for motility wet mounts for motility oxygen requirements Thioglyocallate and TSA Selective and differential medial Temp optimum osmotic effect and ph optimum |
Homework: | |||
7 | Tues. 3/16- Wed. 3/17 |
Metabolic tests (Steubing 16-27) | Homework: | |||
8 | Tues. 3/30- Wed. 3/31 |
Metabolic tests
Antibiotic sensitivity
|
Homework: | |||
9 | Tues. 4/6- Wed. 4/7 |
analysis of rDNA:
practice exercise in lab
|
Homework: | |||
10 | Tues. 4/13- Wed. 4/14 |
lab practical | Homework: . | |||
11 | Tues. 4/20- Wed. 4/22 |
Poster presentation in groups by habitat sampled | Homework: | |||
12 | Tues. 5/4- Wed. 5/5 |
individual data analysis/writing conferences
|
End of lab |