BISC209/S10:Schedule: Difference between revisions
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| Tues. 1/26-<br>Wed. 1/27 | | Tues. 1/26-<br>Wed. 1/27 | ||
| | |Introduction to Microbiology Lab<br> | ||
Lab Safety<br> | |||
Boot camp: Tools and Techniques of Microbiologist: Aseptic Transfer, Streaking for Isolation, Making a bacterial Smear, Gram Stain, Microscopy<br> | |||
Boot camp: | Intro to Microbial Diversity Project:<br> | ||
Tour greenhouse, pick habitat | |||
<br> | |||
Intro to | |||
| | | | ||
| '''Homework''': <br> | | '''Homework''': <br> | ||
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| 2 | | 2 | ||
| Tues. 2/2-<br>Wed. 2/3 | | Tues. 2/2-<br>Wed. 2/3 | ||
| | | Soil sampling Greenhouse habitat <BR> Begin culture of soil organisms on selective, differential and enrichment media<BR> Standard Plate Count of Culturable Soil Organisms<BR> Begin DNA isolation for Non-Culturable Bacterial ID | ||
ENVIRONMENTAL SAMPLING: | ENVIRONMENTAL SAMPLING: | ||
(steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc. | (steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc. | ||
| | | | ||
|'''Homework''' | |'''Homework''' |
Revision as of 07:20, 12 January 2010
BISC209 S10 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday |
---|---|---|---|---|
Jan.25 | Jan. 26 Lab 1 |
Jan. 27 Lab 1 |
Jan. 28 | Jan. 29 |
Feb. 1 | Feb. 2 Lab 2 |
Feb. 3 Lab 2 |
Feb. 4 | Feb. 5 |
Feb. 8 | Feb. 9 Lab 3 |
Feb. 10 Lab 3 |
Feb. 11 | Feb. 12 |
Feb. 15 President's Day |
Feb. 16 Monday schedule NO LAB |
Feb. 17 NO LAB |
Feb. 18 | Feb. 19 |
Feb. 22 | Feb. 23 Lab 4 |
Feb. 24 Lab 4 |
Feb. 25 | Feb. 26 |
Mar. 1 | Mar. 2 Lab 5 |
Mar. 3 Lab 5 |
Mar. 4 | Mar. 5 |
Mar. 8 | Mar. 9 Lab 6 |
Mar. 10 Lab 6 |
Mar. 11 | Mar. 12 |
Mar. 15 | Mar. 16 Lab 7 |
Mar. 17 Lab 7 |
Mar. 18 | Mar. 19 |
Mar. 22 Spring Break |
Mar. 23 Spring Break |
Mar. 24 Spring Break |
Mar. 25 Spring Break |
Mar. 26 Spring Break |
Mar. 29 | Mar. 30 Lab 8 Lab Practical |
Mar. 31 Lab 8 Lab Practical |
Apr. 1 | Apr. 2 |
Apr. 5 | Apr. 6 Lab 9 Data analysis |
Apr. 6 Lab 9< Data Analysis |
Apr. 7 | Apr. 8 |
Apr. 12 | Apr. 13 Lab 10 Conference |
Apr. 14 Lab 10 Conference |
Apr. 15 | Apr. 16 |
Apr. 19 Patriots' Day NO LAB |
Apr. 20 Lab 11 Presentations |
Apr. 21 Lab 11 Presentations |
Apr. 22 Monday Schedule |
Apr. 23 |
Apr. 26 | Apr. 27 NO Lab |
Apr. 28 Rhulman NO Lab |
Apr. 29 | April 30 |
May 3 | May 4 Lab 12 Paper Due |
May 5 Lab 12 Paper Due |
May 6 Last day of classes |
April 30 |
Assignments
BISC110 Assignments/ Weekly Lab Planner
Lab # | LAB DATES__ | IN LAB WORK________ | OUTSIDE OF LAB WORK________ | Assignment/Notes | ||
1 | Tues. 1/26- Wed. 1/27 |
Introduction to Microbiology Lab Lab Safety |
Homework: | |||
2 | Tues. 2/2- Wed. 2/3 |
Soil sampling Greenhouse habitat Begin culture of soil organisms on selective, differential and enrichment media Standard Plate Count of Culturable Soil Organisms Begin DNA isolation for Non-Culturable Bacterial ID ENVIRONMENTAL SAMPLING: (steubing for pop sticks or Jannsen for corers) and isolation streaking in different conditions, temps, media etc. |
Homework | |||
3 | Tues. 2/9- Wed. 2/10 |
DNA diversity: complete prep for pyrosequencing? OR rDNA library steps: (Use soil DNA from lab 2)
DNA library: Clean and PCR Amplify for rDNA using Irene's universal primers (Irene's notes)- 3 hours
techniques: colony morphology, number (see mIcrobial safari= wagner) and problem solve to "discover" 2 isolation methods (serial dilution and pour plates vs streaking for isolation) Isolation to pure colony step: #1: How will they pick what/where on plate to isolate? (secondary plates) Each student picks different 4-6 colonies and restreaks on same media (4-6 different plates). Incubate room temp.grow 24 hours, move to CR.
Control stocks for: gram pos, gram neg and capsule, acid fast and endospore Simple stain Gram stain What do you learn from this? |
Homework: | |||
4 | Tues. 2/23- Wed. 2/24 |
Run gel on PCR from last time
Clone 16s rDNA using TOPO dna cloning kit and MBL lab 12 to complete transformation incubate overnight in plates (see mbl protocol)
ID from secondary cultures (Steubing protocol): on real unknown Techniques: repeat all stains from week before: at least - simple stain (3 pure colonies) gram stain etc (and be sure all stains from week 3 are performed).
Introduce: stains and microscopy: on control stock orgs. -negative stain (for capsules) -acid fast -endospore |
Homework: | |||
5 | Tues. 3/2- Wed. 3/3 |
Select 2 clones and put in 96 well plates (how? protocol ends here) and send them out? where? How long?
UNKNOWN - using stock (1-2 or 3 made) make 2 more stock slants Techniques: all stains: on unknowns (2-3) capsule endospore acid fast etc |
Homework: | |||
6 | Tues.3/9. - Wed. 3/10 |
Unknown:
Physical: Sterbing lab (9-15) soft agar deeps for motility wet mounts for motility oxygen requirements Thioglyocallate and TSA Selective and differential medial Temp optimum osmotic effect and ph optimum |
Homework: | |||
7 | Tues. 3/16- Wed. 3/17 |
Metabolic tests (Steubing 16-27) | Homework: | |||
8 | Tues. 3/30- Wed. 3/31 |
Metabolic tests
Antibiotic sensitivity
|
Homework: | |||
9 | Tues. 4/6- Wed. 4/7 |
analysis of rDNA:
practice exercise in lab
|
Homework: | |||
10 | Tues. 4/13- Wed. 4/14 |
lab practical | Homework: . | |||
11 | Tues. 4/20- Wed. 4/22 |
Poster presentation in groups by habitat sampled | Homework: | |||
12 | Tues. 5/4- Wed. 5/5 |
individual data analysis/writing conferences
|
End of lab |