BISC209/S10:Schedule

BISC209 S10 Lab Calendar

Monday	Tuesday	Wednesday	Thursday	Friday
Jan.25	Jan. 26 Lab 1	Jan. 27 Lab 1	Jan. 28	Jan. 29
Feb. 1	Feb. 2 Lab 2	Feb. 3 Lab 2	Feb. 4	Feb. 5
Feb. 8	Feb. 9 Lab 3	Feb. 10 Lab 3	Feb. 11	Feb. 12
Feb. 15 President's Day	Feb. 16 Monday schedule NO LAB	Feb. 17 NO LAB	Feb. 18	Feb. 19
Feb. 22	Feb. 23 Lab 4	Feb. 24 Lab 4	Feb. 25	Feb. 26
Mar. 1	Mar. 2 Lab 5	Mar. 3 Lab 5	Mar. 4	Mar. 5
Mar. 8	Mar. 9 Lab 6	Mar. 10 Lab 6	Mar. 11	Mar. 12
Mar. 15	Mar. 16 Lab 7	Mar. 17 Lab 7	Mar. 18	Mar. 19
Mar. 22 Spring Break	Mar. 23 Spring Break	Mar. 24 Spring Break	Mar. 25 Spring Break	Mar. 26 Spring Break
Mar. 29	Mar. 30 Lab 8 Lab Practical	Mar. 31 Lab 8 Lab Practical	Apr. 1	Apr. 2
Apr. 5	Apr. 6 Lab 9 Data analysis	Apr. 6 Lab 9< Data Analysis	Apr. 7	Apr. 8
Apr. 12	Apr. 13 Lab 10 Conference	Apr. 14 Lab 10 Conference	Apr. 15	Apr. 16
Apr. 19 Patriots' Day NO LAB	Apr. 20 Lab 11 Presentations	Apr. 21 Lab 11 Presentations	Apr. 22 Monday Schedule	Apr. 23
Apr. 26	Apr. 27 NO Lab	Apr. 28 Rhulman NO Lab	Apr. 29	April 30
May 3	May 4 Lab 12 Paper Due	May 5 Lab 12 Paper Due	May 6 Last day of classes	April 30

Assignments

BISC110 Assignments/ Weekly Lab Planner

Lab #	LAB DATES__	IN LAB WORK________	OUTSIDE OF LAB WORK________	Assignment/Notes
1	Tues. 1/26- Wed. 1/27	Introduction to Microbiology Lab Lab Safety Boot camp: Tools and Techniques of Microbiologist: Aseptic Transfer, Streaking for Isolation, Making a bacterial Smear, Gram Stain, Microscopy Intro to Microbial Diversity Project: Tour greenhouse, pick habitat		Homework: Read reference articles on bacterial soil diversity & culturing soil bacteria. References posted to the Lab First Class conference (Reference Folder)
2	Tues. 2/2- Wed. 2/3	Soil sampling in Greenhouse habitat Begin culture of soil organisms on selective, differential and enrichment media Standard Plate Count of Culturable Soil Organisms Begin DNA isolation for Non-Culturable Bacterial ID	Check on your soil bacterial enrichment and selection cultures; Assess your isolation streaking; check on your plate count plates and move any in danger of overgrowing to cold room	Homework
3	Tues. 2/9- Wed. 2/10	DNA diversity: complete prep for pyrosequencing? OR rDNA library steps: (Use soil DNA from lab 2) DNA library: Clean and PCR Amplify for rDNA using Irene's universal primers (Irene's notes)- 3 hours CULTURES: (Primary plates) observe-count, describe, select soil microbes for further culturing (compare anaerobic, aerobic, microaerophillic) - search web for pics of microbes. techniques: colony morphology, number (see mIcrobial safari= wagner) and problem solve to "discover" 2 isolation methods (serial dilution and pour plates vs streaking for isolation) Isolation to pure colony step: #1: How will they pick what/where on plate to isolate? (secondary plates) Each student picks different 4-6 colonies and restreaks on same media (4-6 different plates). Incubate room temp.grow 24 hours, move to CR. Microscopy introduced: select something that looks isolated and stain and compare to stock cultures. Control stocks for: gram pos, gram neg and capsule, acid fast and endospore Simple stain Gram stain What do you learn from this?		Homework:
4	Tues. 2/23- Wed. 2/24	Run gel on PCR from last time Clone 16s rDNA using TOPO dna cloning kit and MBL lab 12 to complete transformation incubate overnight in plates (see mbl protocol) Cultures: Examine and pick 3 isolated colonies (if needed) additional technniques: ID from secondary cultures (Steubing protocol): on real unknown Techniques: repeat all stains from week before: at least - simple stain (3 pure colonies) gram stain etc (and be sure all stains from week 3 are performed). make stocks from (3) isolated cultures- make 2 fresh slants per organism (each person) Introduce: stains and microscopy: on control stock orgs. -negative stain (for capsules) -acid fast -endospore		Homework:
5	Tues. 3/2- Wed. 3/3	Select 2 clones and put in 96 well plates (how? protocol ends here) and send them out? where? How long? UNKNOWN - using stock (1-2 or 3 made) make 2 more stock slants Techniques: all stains: on unknowns (2-3) capsule endospore acid fast etc		Homework:
6	Tues.3/9. - Wed. 3/10	Unknown: Physical: Sterbing lab (9-15) soft agar deeps for motility wet mounts for motility oxygen requirements Thioglyocallate and TSA Selective and differential medial Temp optimum osmotic effect and ph optimum		Homework:
7	Tues. 3/16- Wed. 3/17	Metabolic tests (Steubing 16-27)		Homework:
8	Tues. 3/30- Wed. 3/31	Metabolic tests Antibiotic sensitivity		Homework:
9	Tues. 4/6- Wed. 4/7	analysis of rDNA: practice exercise in lab Optional complete/redo any tests not completed		Homework:
10	Tues. 4/13- Wed. 4/14	lab practical		Homework: .
11	Tues. 4/20- Wed. 4/22	Poster presentation in groups by habitat sampled		Homework:
12	Tues. 5/4- Wed. 5/5	individual data analysis/writing conferences		End of lab

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12