BISC209/S11:Assignments

Lab Graded Assignments

Lab Assigned	Lab Due	Assignment description	Point Value (Total 200)
1	2	Turn in at the beginning of Lab 2 a 2-3 page Discussion with References of how the enrichment culture techniques and media you will use in Labs 1& 2 will select soil bacteria of the specific groups we seek from other microbes in the community. Be sure to read the directions for this assignment found at Assignment: Enrichment for Culturable Bacteria of specific groups.	5
2	3	Make a Table of the relevant morphologic (shape, arrangement, Gram stain, motility etc) and useful metabolic characteristics of expected genera or soil bacterial from some of the groups (spore formers, antibiotic producers, nitrogen cyclers, denitrifiers) that you are attempting to find in your habitat.	5
3	4	M&M Amplification of 16S rDNA in a soil sample	5
4	5	Introduction section of final paper. Read over the Introduction to the Project page in the wiki to identify the topic and experimental questions addressed. Include the history of the "Great Plate Count Anomaly" (the disparity between culturable and unculturable soil community bacteria). Use and cite references in journal Cell format. A reference that may be helpful is :| Uncultivated Microorganisms by Slava Epstein in Microbiology Monographs Vol. 10, 2009 DOI: 10.1007/978-3-540-85465-4 available as an e-book through Springerlink at the Wellesley College Library or as a pdf file in the Resources section of the lab Sakai site.	15
5	6	M&M Isolation of soil bacteria to pure culture & Identification of bacteria by 16S rRNA gene sequencing from soil genomic DNA	5
6	7	Write a brief summary of the theory behind the following techniques that we used to identify our bacterial species by molecular tools: genomic DNA isolation, polymerase chain amplification of part of the 16s rRNA genes, use of the Zero Blunt® TOPO® PCR Cloning Kit to create a library of unique plasmid vector with our 16S rRNA gene inserts and then select, One Shot® TOP10 Competent E. coli Cells that allowed us to select and separate our 16S rRNA genes for sequencing, and DNA sequencing by the Sanger method.	5
7	8	Study for Lab Practical
8	8	Lab practical	45
8	9	Results section with figures/tables: Identification of Culturable Soil Bacteria by Physical and Metabolic Characteristics	10
9	10	Partial Results section with figure(s)/table(s) Soil Community Diversity Results from 16S rDNA sequencing	10
10	11	Group "Virtual" Poster Presentation Note that the 25pt includes 10 points of Individual Contribution to Group points--assigned by consensus of your project and presentation group	25
11	12	Lab notebook	5
12	12	Final Paper	60
Other		CLEAN-UP points	5

Assignments

Lab 1 Assignment: Assignment: Enrichment for culturable bacteria of specific groups
Lab 2 Assignment: Assignment2 ????
Lab 3 Assignment: Assignment3 ????
Lab 4 Assignment: Assignment4 ????
Lab 5 Assignment: Assignment5 ???
Lab 6 Assignment: Assignment6 ???
Lab 7 Assignment: Assignment7 ???
Lab 8 Assignment: Assignment8 ???
Lab 9 Assignment: Assignment9 ???
Lab 10 Assignment: Assignment10 ???
Lab 11 Assignment: Assignment11 ???
Lab 12 Assignment: Assignment12 ???

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12