BISC209/S13:Schedule

BISC209 S13 Lab Calendar

Monday	Tuesday	Wednesday	Thursday	Friday
Jan.28 Classes begin	Jan. 29 NO LAB	Jan. 30 NO LAB	Jan. 31	Feb. 1
Feb 4	Feb. 5 Lab 1	Feb. 6 Lab 1	Feb. 7	Feb. 8
Feb. 11	Feb. 12 Lab 2	Feb. 13 Lab 2	Feb. 14	Feb. 15
Feb. 18 Presidents' Day NO CLASSES	Feb. 19 NO LAB Monday Schedule	Feb. 20 NO LAB	Feb. 21	Feb. 22
Feb. 25	Feb. 26 Lab 3	Feb. 27 Lab 3	Feb. 28	Mar. 1
Mar. 4	Mar. 5 Lab 4	Mar. 6 Lab 4	Mar. 7	Mar. 8
Mar. 11	Mar. 12 Lab 5	Mar. 13 Lab 5	Mar. 14	Mar. 15
Mar. 18 Spring Break	Mar. 19 Spring Break	Mar. 20 Spring Break	Mar. 21 Spring Break	Mar. 22 Spring Break
Mar. 25	Mar. 26 Lab 6	Mar. 27 Lab 6	Mar. 28	Mar. 29
Apr. 1	Apr. 2 Lab 7	Apr. 3 Lab 7	Apr. 4	Apr. 5
Apr. 8	Apr. 9 Lab 8	Apr. 10 Lab 8	Apr. 11	Apr. 12
Apr. 15 Patriots' Day	Apr. 16 Lab 9 Maldi-TOF Biotyper ID	Apr. 17 Lab 9 Maldi-TOF Biotyper ID	Apr. 18	Apr. 19
Apr. 22	Apr. 23 No Lab	Apr. 24 Rhulman NO LAB	Apr. 25	Apr. 26
Apr. 29	Apr. 30 Lab 10 Lab Practical and Final Paper Consultation	May 1 Lab 10 Lab Practical and Final Paper Consultation	May 2	May 3
May. 6	May 7 NO LAB Tues Lab Paper Due	May 8 NO Lab Wed Lab Paper Due Last day of classes	May 9	May 10

Weekly Topics

BISC209 Weekly Lab Topics

Lab #	LAB DATES__	IN LAB WORK__________________	OUTSIDE OF LAB WORK________
1	Tues. 2/5- Wed. 2/6	Introduction to Microbiology Lab Lab Safety Tools and Techniques of Microbiologist: Aseptic Transfer, Intro to Soil Microbial Community Project:Soil sampling in Greenhouse habitat;Begin culture of soil organisms: make soil extract and begin enrichment for selected bacteria; Start Plate Count of Culturable Soil Organisms	Visit the greenhouse and make notes on your selected habitat.
2	Tues. 2/12- Wed. 2/13	Finish Plate Count & quantify cultured microbes by culture dependent method; Practice Streaking for Isolation; Make soil extract from dried soil and set up isolation of spore-forming bacteria; Continue enrichment & isolation of selected groups of bacteria	Assess your isolation streaking and continue to isolation streak to pure culture. ON the MORNING OF LAB 3 Collect A NEW SOIL SAMPLE from your group's sampling site. (Materials available for pick up in the lab.)
3	Tues. 2/26- Wed. 2/27	Isolation of Culturable Bacteria. Evaluate streaking for isolation. Make another soil extract and serial dilution Start CLPP: Community Level Physiological Profiling: Carbon source utilization; Continue selection & isolation, of desired bacterial groups; Community exoexyzme profiling (starch & cellulose digesters, phosphate solubilizers).	Collect data from BIOLOG ECO plates;. Check on cultures and continue isolation to pure culture.
4	Tues. 3/5- Wed. 3/6	Isolation of Culturable Bacteria: Examine cultures and pick unique isolated colonies of your soil bacteria Community Exoenzyme assessment; CLPP analysis and calculations of carbon source utilization	Complete isolation to pure culture and set up fresh pure cultures 24-48 hours prior to next lab.
5	Tues. 3/12- Wed. 3/13	Culturable Bacteria: Make new cultures from each of your pure cultures of your soil bacteria isolates. Use a pure culture of each isolate to its amplify 16srRNA gene by pcr. Clean up pcr product. Perform agarose gel electrophoresis of pcr product to assess success of amplification.	Set up fresh isolate cultures 24-48 hours prior to next lab as needed.
6	Tues. 3/26 - Wed. 3/27	Isolate Testing: Perform physical characteristics tests: smear slide, Gram stain, confirm Gram stain with selective media, start an antibiotic production test.	Set up fresh isolate cultures 24-48 hours prior to next lab as needed.
7	Tues. 4/2- Wed. 4/3	Isolates Testing: Start motility test and MNM tests; Continue antibiotic production test; Read Gram stain confirmation by selective media, Start Bacterial interactions test.	Set up fresh isolate cultures 24-48 hours prior to next lab.
8	Tues. 4/9- Wed. 4/10	Cont. Isolates testing: Complete and read antibiotic production test, interactions, MNM tests. Special Staining as indicated.	Set up fresh isolate cultures 24 hours prior to next lab for freezing.
9	Tues. 4/16- Wed. 4/17	Maldi-TOF biotyper ID of isolates and freezing Isolates	Set up fresh isolate cultures if needed.
10	Tues. 4/23- Wed. 4/24 RHULMAN	No Formal LABS - Freeze isolates if not completed in lab 9
11	Tues. 4/30- Wed. 5/1	Lab Practical and Paper Rough Draft
12	Tues. 5/7- Wed. 5/8	PAPER DUE	Final Paper Due by 5pm uploaded to Sakai AND in hard copy to your instructor's office if she requests it.