BISC209/S13: Assignment 209 Lab8: Difference between revisions
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==Lab 8: Isolate structure and functional capacity table== | ==Lab 8: Isolate structure and functional capacity table== | ||
Construct a Table for your final paper on the isolates from your soil community (your isolates and your teammates' isolates from the same sampling site) and write a complete results narrative. You should include in the table: The isolate ID you used all semester, Gram stain, description of the colony morphology, description of the individual bacteria's structural characteristics including shape & arrangement (cocci, rods, with descriptors ie., large, small, bullet shaped, in chains, etc.), evidence of spores (either endospore stain positive or visualization of empty areas in vegetative cells on Gram stain), motility, etc. Include test results that you will use as evidence for metabolic diversity or community behavior, ie. nitrogen cycling capabilities, fermentation of mannitol, etc. Make separate figures from photographs of the interactions assays for your soil sample and another from a photo of a positive antibiotic production assay (if your soil sample showed such a positive) and analyze those data in your narrative. | Construct a Table for your final paper on the isolates from your soil community (your isolates and your teammates' isolates from the same sampling site) and write a complete results narrative. You should include in the table: The isolate ID you used all semester, Gram stain, description of the colony morphology, description of the individual bacteria's structural characteristics including shape & arrangement (cocci, rods, with descriptors ie., large, small, bullet shaped, in chains, etc.), evidence of spores (either endospore stain positive or visualization of empty areas in vegetative cells on Gram stain), motility, etc. Include test results that you will use as evidence for metabolic diversity or community behavior, ie. nitrogen cycling capabilities, fermentation of mannitol, etc. Make separate figures from photographs of the interactions assays for your soil sample and another from a photo of a positive antibiotic production assay (if your soil sample showed such a positive) and analyze those data in your narrative. Complete a google doc with the data for your site: Design a table to use in your paper and post it in the folder for your soil sampling site so your classmates can access and use this information for the other bacterial members of each soil community. Leave space in the table to add the scientific name of your isolate once the identification is back from GeneWiz. | ||
The results analysis narrative for the table of tests performed on the isolates might start out, "In order to show structural and metabolic diversity as well as the potential for community behavior (co-operation and/or competition) among members of a soil community (the goal), nitrogen cycling bacteria & spore-forming bacteria were enriched and selected using media and protocols described in Materials and Methods. Once in pure culture a few soil community members of these groups and other interesting bacteria isolated on non-selective media were visualized using staining techniques for cell wall structure in a Gram stain, production of endospores with Shaeffer-Fulton endospore stain, the presence of capsules with a negative nigrosin stain, and flagella with a flagella stain. Additionally motility testing was performed on each isolate using soft agar deeps (the tools). Evidence for a role in the nitrogen cycle was assessed by________. (continue to describe any other testing you include in your table)......Table 1 shows/supports/indicates _________ (analysis of results)." <BR> |
Revision as of 18:41, 14 January 2013
Lab 8: Isolate structure and functional capacity table
Construct a Table for your final paper on the isolates from your soil community (your isolates and your teammates' isolates from the same sampling site) and write a complete results narrative. You should include in the table: The isolate ID you used all semester, Gram stain, description of the colony morphology, description of the individual bacteria's structural characteristics including shape & arrangement (cocci, rods, with descriptors ie., large, small, bullet shaped, in chains, etc.), evidence of spores (either endospore stain positive or visualization of empty areas in vegetative cells on Gram stain), motility, etc. Include test results that you will use as evidence for metabolic diversity or community behavior, ie. nitrogen cycling capabilities, fermentation of mannitol, etc. Make separate figures from photographs of the interactions assays for your soil sample and another from a photo of a positive antibiotic production assay (if your soil sample showed such a positive) and analyze those data in your narrative. Complete a google doc with the data for your site: Design a table to use in your paper and post it in the folder for your soil sampling site so your classmates can access and use this information for the other bacterial members of each soil community. Leave space in the table to add the scientific name of your isolate once the identification is back from GeneWiz.
The results analysis narrative for the table of tests performed on the isolates might start out, "In order to show structural and metabolic diversity as well as the potential for community behavior (co-operation and/or competition) among members of a soil community (the goal), nitrogen cycling bacteria & spore-forming bacteria were enriched and selected using media and protocols described in Materials and Methods. Once in pure culture a few soil community members of these groups and other interesting bacteria isolated on non-selective media were visualized using staining techniques for cell wall structure in a Gram stain, production of endospores with Shaeffer-Fulton endospore stain, the presence of capsules with a negative nigrosin stain, and flagella with a flagella stain. Additionally motility testing was performed on each isolate using soft agar deeps (the tools). Evidence for a role in the nitrogen cycle was assessed by________. (continue to describe any other testing you include in your table)......Table 1 shows/supports/indicates _________ (analysis of results)."