BISC209:Protcols

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(Microbiology Protocols)
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(Microbiology Protocols)
 
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==Microbiology Protocols==
==Microbiology Protocols==
[[BISC209: Agar Overlay | Agar Overlay (Pour) Plates ]]<BR>
[[BISC209: Agar Overlay | Agar Overlay (Pour) Plates ]]<BR>
-
[[BISC209: Aseptic Transfer |  Aseptic Transfer ]]<BR>
+
[[BISC209: Aseptic Transfer |  Aseptic Transfer: Making Slants, Broth to Broth or Plate Transfer ]]<BR>
[[BISC209: Calibration of Micropipets | Calibration of Micropipets ]]<BR>
[[BISC209: Calibration of Micropipets | Calibration of Micropipets ]]<BR>
 +
[[BISC209: Carbohydrate Fermentation Medium|Carbohydrate Fermentation Medium]]<BR>
 +
[[BISC209: Enrichment | Enrichment Media for Soil Bacterial from Mixed Populations ]]<BR>
[[BISC209: Enterotube | Enterotube Protocol]]<BR>
[[BISC209: Enterotube | Enterotube Protocol]]<BR>
-
[[BISC209: Carbohydrate Fermentation Medium]]<BR>
 
[[BISC209: Enzyme tests |Enzyme tests ]]<BR>
[[BISC209: Enzyme tests |Enzyme tests ]]<BR>
[[BISC209: The Gram Stain | Gram Stain]]<BR>
[[BISC209: The Gram Stain | Gram Stain]]<BR>
[[BISC209: Microscopy | Microscopy: Care and Use of the Compound Brightfield Microscope]]<BR>
[[BISC209: Microscopy | Microscopy: Care and Use of the Compound Brightfield Microscope]]<BR>
[[BISC209: Motility | Motility Tests]]<BR>
[[BISC209: Motility | Motility Tests]]<BR>
-
[[BISC209: Pour Plates | Pour Plates ]]<BR>
+
[[BISC209: Pour Plates | Pouring Media To Make Solid Agar Plates ]]<BR>
 +
[[BISC209: Rhizobium isolation from soil |Rhizobium isolation from soil]]<BR>
[[BISC209: Serial Dilution | Serial Dilution ]]<BR>
[[BISC209: Serial Dilution | Serial Dilution ]]<BR>
-
[[BISC209: Simple Stains | Simple staining technique]]<BR>
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[[BISC209: Simple Stains | Simple Stains]]<BR>
[[BISC209: Preparing a bacterial smear slide | Smear Slide Preparation]]<BR>
[[BISC209: Preparing a bacterial smear slide | Smear Slide Preparation]]<BR>
-
[[BISC209: Stains | Special Stains: Endospore, Acid fast, Capsule, and Flagella]]<BR>
+
[[BISC209: Recipes | Recipes for test medium and reagents]]<BR>
 +
 
 +
[[BISC209: Stock cultures | Stock cultures ]]<BR>
 +
[[BISC209: Stains | Stains (Special): Endospore, Acid fast, Capsule, and Flagella]]<BR>
[[BISC209: Streaking for Isolation | Streaking for Isolation ]]<BR>
[[BISC209: Streaking for Isolation | Streaking for Isolation ]]<BR>
-
[[BISC209: Enrichment | Targeted Enrichment Media for Soil Bacterial Selection from Mixed Populations ]]<BR>
+
[[BISC209: Roles of soil Microbes |Tests to determine the role of a soil isolate]]<BR>
[[BISC209: Selective,and/or Differential, and/or Enriched media |Use of Selective, Differential, Enrichment Media]]<BR>
[[BISC209: Selective,and/or Differential, and/or Enriched media |Use of Selective, Differential, Enrichment Media]]<BR>
-
[[BISC209: Rhizobium isolation from soil |Isolation of Rhizobium from soil]]<BR>
+
 
-
[[BISC209: Streptomyces isolation from soil |Isolation of Streptomyces from soil]]<BR>
+
[[BISC209: wet mounts | Wet Mount procedure ]]<BR>
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[[BISC209: Roles of soil Microbes |Protocols for determining the roles of soil isolates in the soil]]<BR>
+
 
 +
==Links to Labs==
 +
[[BISC209:BootCamp | Lab 1 ]]<br>
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[[BISC209: Lab2 | Lab 2 ]]<br>
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[[BISC209: Lab3 | Lab 3 ]]<br>
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[[BISC209: Lab4 | Lab 4 ]]<br>
 +
[[BISC209: Lab5 | Lab 5 ]]<br>
 +
[[BISC209: Lab6 | Lab 6 ]]<br>
 +
[[BISC209: Lab7 | Lab 7 ]]<br>
 +
[[BISC209: Lab8 | Lab 8 ]]<br>
 +
[[BISC209: Lab9 | Lab 9 ]]<br>
 +
[[BISC209: Lab10 | Lab 10 ]]<br>
 +
[[BISC209: Lab11 | Lab11 ]]<br>
 +
[[BISC209: Lab12 | Lab 12 ]]<br>

Current revision

Wellesley College-BISC 209 Microbiology -Spring 2010

Microbiology Protocols

Agar Overlay (Pour) Plates
Aseptic Transfer: Making Slants, Broth to Broth or Plate Transfer
Calibration of Micropipets
Carbohydrate Fermentation Medium
Enrichment Media for Soil Bacterial from Mixed Populations
Enterotube Protocol
Enzyme tests
Gram Stain
Microscopy: Care and Use of the Compound Brightfield Microscope
Motility Tests
Pouring Media To Make Solid Agar Plates
Rhizobium isolation from soil
Serial Dilution
Simple Stains
Smear Slide Preparation
Recipes for test medium and reagents

Stock cultures
Stains (Special): Endospore, Acid fast, Capsule, and Flagella
Streaking for Isolation
Tests to determine the role of a soil isolate
Use of Selective, Differential, Enrichment Media

Wet Mount procedure

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11

Lab 12
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