BISC209: Calibration of Micropipets

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Wellesley College-BISC 209 Microbiology -Spring 2010

Protocol for Micropipet Calibration

1. To calibrate your P1000, P 200, and P 20 micropipets, label 6 microfuge tubes (1-6) and weigh them on a top loading balance. Don't forget to tare the balance. Record the weights in a table in your lab notebook, like the one below.

2. Using the information in the table below, pipet the specified volumes into the pre-weighed microfuge tubes prepared in step 1 and then reweigh the tubes. Record all weights.

3. Calculate the weight of the water in grams by substracting the dry weight from the weight of the tubes with water. Note that 1000 microliters of water should weigh exactly 1 gram at room temperature.

4. If the water in any tube weighs more or less than 1 gram, repeat that tube. Ask your instructor to check your pipetting technique if your calibration continues to be off after several repeated attempts.


Tube # Pre-weight Tube Vol.
in µl using P20
Vol. in µl
using P200
Vol. in µl
using P1000
Weight of water
grams
1 _____ 10 0 990 ____
2 _____ 0 100 900 ____
3 _____ 20 175 805 _____
4 _____ 2 88 910 ______
5 _____ 0 200 x 5 0 _____
6 _____ 20 x 5 0 900 _____

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11

Lab 12
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