BISC209: Enterotube

Enterotube inoculation Protocol

1. Examine the ENTEROTUBE™ Note the following components: 1) caps on each end of the test system; 2) the different compartments; 3) the inoculating needle within the system.

2. Label the ENTEROTUBE™ and select a fresh, isolated colony from one of the unknown culture plates.

3. Remove both end caps of the system, and without flaming the protruding inoculating needle; pickup a colony* directly onto its tip. Try not to touch the agar with the wire.

4. Next, with a turning motion twist and pull the needle through all compartments of the system. This action will serve to inoculate the ENTEROTUBE™.

5. Reinsert the needle (without flaming) into the system, again using a twisting motion. Stop when the needle's tip is seen in the citrate compartment.

6. A notch on the needle should be evident now. Break the needle at this point by bending it. The portion of the needle remaining in some of the compartments will maintain the required anaerobic conditions.

7. Using the broken off piece of wire, punch holes in the covering of the other 8 compartments to provide aerobic conditions for these tests.

8. Incubate the ENTEROTUBE™ at 37˚C with the tube lying on a flat surface for 24 hours.

9. After incubation, examine the ENTEROTUBE™. Positive reactions for the different compartments are shown in the pamphlet explaining the ENTEROTUBE™ test, which is posted in the lab.

The BBL Enterotube

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12