BISC209: Lab5

LAB 5: Con't. Project: Soil Microbial Communities & Diversity

Identification of your cultured soil bacteria from your pure colonies

ID Cultured Bacteria by 16srDNA Sequencing and Analysis

Identify Bacteria from Genomic DNA from Isolated Pure Colonies

Choose 4 different isolated bacterial colonies per person

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Lyse Cells by boiling
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PCR amplify 16srDNA with the bacterial primers: 8F and 1492R

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Visualize PCR product by agarose getl electrophoresis ⇓
Prepare PCR product dor DNA sequencing with ExoSap
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Submit PCR products for DNA sequencing

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Determine id of cultured and isolated soil organisms with sequence comparisons to database