LAB 5: Con't. Project: Soil Microbial Communities & Diversity
Identification of your cultured soil bacteria from your pure colonies
Molecular Strategy for Indentification of Culturable & Non-culturable Bacteria in a Soil Sample
Identify Bacteria from Genomic DNA from Isolated Pure Colonies
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Choose 8 isolated bacterial colonies per person
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Lyse Cells by boiling
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PCR amplify 16srDNA with the bacterial primers: 8F and 1492R
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Visualize PCR product by agarose getl electrophoresis
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Prepare PCR product dor DNA sequencing with ExoSap
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Submit PCR products for DNA sequencing
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Determine id of cultured and isolated soil organisms with sequence comparisons to database
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