BISC209: Lab9

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Wellesley College-BISC 209 Microbiology -Spring 2010


LAB 9: Data Analysis Lab

We will learn how to use the RDB (ribosomal data base, a public data base provided by Michigan State University) to analyze your sequencing results. Lab to be held in a computer classroom (TBA). Make sure you have signed up for an account on the RDB and received a username and password before you come to lab. Link to the RDB:
[ http://rdp.cme.msu.edu/index.jsp]

Click on myRDP (orange sidebar on the left of the screen).

Click on "Not a user? Sign up!" and this will take you to a registration screen. Fill out the information required and then check your email. You will be sent a password. Go back to the myRDP site and login with your email and this password. It will then prompt you to change that password to something you can remember.

WRITE THIS DOWN! Don't lose your password!

Assignment

Write another results section with figure(s) and/or table(s): Soil community diversity identification from 16S rDNA sequencing: phylogenetic relationships and their significance. Due next week at your conference with your instructor.

Please come in your habitat groups of 3 or 4 students to your lab instructor's office for a 30 minute conference about your data analysis for your poster presentation. Have a draft of all of your figures prepared to show me and a written summary of your discussion points and conclusions. There will be time for you to ask questions but, primarily, think of this conference as presenting to me a draft of your presentation and a preview of your paper.

The assignment information in LAB 8 and feedback from your previous graded results section assignment should help you with this assignment.

Continue to wind down and finalize the classification of your culturable isolates. Start discarding your cultures.

Link to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11

Lab 12
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