BISC209: Recipes: Difference between revisions
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==Misc Recipes for test media and their reagents== | ==Misc Recipes for test media and their reagents== | ||
'''SIM agar and Reagent recipes:'''< | '''SIM agar and Reagent recipes:'''<UL><LI> | ||
'''SIM agar:'''< | '''SIM agar:'''<LI> | ||
Approximate Formula* Per Liter<BR> | Approximate Formula* Per Liter<BR> | ||
Pancreatic Digest of Casein - 20.0 g<BR> | Pancreatic Digest of Casein - 20.0 g<BR> | ||
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Ferrous Ammonium Sulfate - 0.2 g<BR> | Ferrous Ammonium Sulfate - 0.2 g<BR> | ||
Sodium Thiosulfate - 0.2 g<BR> | Sodium Thiosulfate - 0.2 g<BR> | ||
Agar - 3.5 g< | Agar - 3.5 g<LI> | ||
'''Kovac's reagent: (per liter)'''<BR> | '''Kovac's reagent: (per liter)'''<BR> | ||
p-Dimethylaminobenzaldehyde 50,0g<BR> | p-Dimethylaminobenzaldehyde 50,0g<BR> | ||
Amyl Alcohol 750.0 ml<BR> | Amyl Alcohol 750.0 ml<BR> | ||
Hydrochloric acid 250.0 ml< | Hydrochloric acid 250.0 ml<LI> | ||
'''Indole Spot Test Reagent'''<BR> | '''Indole Spot Test Reagent'''<BR> | ||
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Hydrochloric acid 100.0ml <BR> | Hydrochloric acid 100.0ml <BR> | ||
Water, deionized 900.0 ml<BR> | Water, deionized 900.0 ml<BR> | ||
</LI></UL> | |||
Revision as of 10:06, 18 January 2010
Misc Recipes for test media and their reagents
SIM agar and Reagent recipes:
- SIM agar:
-
Approximate Formula* Per Liter
Pancreatic Digest of Casein - 20.0 g
Peptic Digest of Animal Tissue - 6.1 g
Ferrous Ammonium Sulfate - 0.2 g
Sodium Thiosulfate - 0.2 g
Agar - 3.5 g -
Kovac's reagent: (per liter)
p-Dimethylaminobenzaldehyde 50,0g
Amyl Alcohol 750.0 ml
Hydrochloric acid 250.0 ml -
Indole Spot Test Reagent
p-Dimethylaminocinnamaldehyde 10.0 g
Hydrochloric acid 100.0ml
Water, deionized 900.0 ml
