BISC209: Recipes: Difference between revisions
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15 g Agar<BR> | 15 g Agar<BR> | ||
final pH 6.9-7.3 <BR><BR></LI></UL> | final pH 6.9-7.3 <BR><BR></LI></UL> | ||
Lab 5 | |||
'''catalase test'''<BR> | |||
fresh hydrogen peroxide<BR | |||
<BR> | |||
'''Oxidase test'''<BR> | |||
order fresh commercial kits <BR> | |||
postitive controls: Bacillus spp, Neisseria, Micrococcus or Pseudomonas) | |||
Lab 6 | Lab 6 | ||
'''MRVP'''Methyl red test<UL><LI> | |||
dextrose peptone broth <BR> | |||
0.7% peptone,<BR> | |||
0.5% dextrose<BR> | |||
0.5% dipotassium phosphate<BR> | |||
pH 6.7-7.1</LI></UL> | |||
Methyl red Reagent <UL><LI> | |||
0.1 methyl red dye<BR> | |||
ethanol 00 ml<BR> | |||
distilled water to 500 ml</LI></UL> | |||
VP Reagent A <UL><LI> | |||
5 g alpha naphthol<BR> | |||
bring volume to 100 ml absolute alcohol</LI></UL> | |||
VP Reagent B<UL><LI> | |||
40g potassium hydroxide<BR> | |||
bring to volume with 100 ml distilled water</LI></UL> | |||
'''SIM agar and Reagent recipes:'''<UL><LI> | '''SIM agar and Reagent recipes:'''<UL><LI> | ||
Revision as of 11:46, 18 January 2010
Misc Recipes for test media and their reagents
Lab 4:
PEA MEDIUM-
10g tryptose
3g Beef extract
5g Sodium Chloride
2.5g Phenylethyl alcohol
15g Agar to 1 liter distilled or deionized water
pH 7.1-7.5.
-
10g peptone
10g Lactose
2g dipotassium phosphate
0.4g eosin Y
0.065 g methylene blue
15 g Agar
final pH 6.9-7.3
Lab 5
catalase test
fresh hydrogen peroxide<BR
Oxidase test
order fresh commercial kits
postitive controls: Bacillus spp, Neisseria, Micrococcus or Pseudomonas)
Lab 6
MRVPMethyl red test-
dextrose peptone broth
0.7% peptone,
0.5% dextrose
0.5% dipotassium phosphate
pH 6.7-7.1
-
0.1 methyl red dye
ethanol 00 ml
distilled water to 500 ml
-
5 g alpha naphthol
bring volume to 100 ml absolute alcohol
-
40g potassium hydroxide
bring to volume with 100 ml distilled water
-
Approximate Formula* Per Liter
Pancreatic Digest of Casein - 20.0 g
Peptic Digest of Animal Tissue - 6.1 g
Ferrous Ammonium Sulfate - 0.2 g
Sodium Thiosulfate - 0.2 g
Agar - 3.5 g -
Kovac's reagent: (per liter)
p-Dimethylaminobenzaldehyde 50,0g
Amyl Alcohol 750.0 ml
Hydrochloric acid 250.0 ml -
Indole Spot Test Reagent
p-Dimethylaminocinnamaldehyde 10.0 g
Hydrochloric acid 100.0ml
Water, deionized 900.0 ml
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
