BISC209: Recipes: Difference between revisions
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==Misc Recipes for test media and their reagents== | ==Misc Recipes for test media and their reagents== | ||
Lab 4 | Lab 4<BR> | ||
'''PEA MEDIUM'''<UL><LI> | '''PEA MEDIUM'''<UL><LI> | ||
10g tryptose<BR> | 10g tryptose<BR> | ||
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final pH 6.9-7.3 <BR><BR></LI></UL> | final pH 6.9-7.3 <BR><BR></LI></UL> | ||
Lab 5 | Lab 5<BR> | ||
'''catalase test'''<BR> | '''catalase test'''<BR> | ||
fresh hydrogen peroxide<BR | fresh hydrogen peroxide<BR> | ||
<BR> | <BR> | ||
'''Oxidase test'''<BR> | '''Oxidase test'''<BR> | ||
order fresh commercial kits <BR> | order fresh commercial kits <BR> | ||
positive controls: Bacillus spp, Neisseria, Micrococcus or Pseudomonas<BR><BR> | |||
'''OF-glucose medium'''<BR> | '''OF-glucose medium'''<BR> | ||
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It is toxic if swallowed, inhaled or absorbed through the skin. It presents a neurological hazard and may act as a carcinogen and be a reproductive hazard. It is corrosive and causes burns. | It is toxic if swallowed, inhaled or absorbed through the skin. It presents a neurological hazard and may act as a carcinogen and be a reproductive hazard. It is corrosive and causes burns. | ||
Wear gloves and consider performing this assay in a hood.</LI></UL> | Wear gloves and consider performing this assay in a hood.</LI></UL><BR> | ||
Lab 6<BR> | Lab 6<BR> | ||
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0.1 methyl red dye<BR> | 0.1 methyl red dye<BR> | ||
ethanol 00 ml<BR> | ethanol 00 ml<BR> | ||
distilled water to 500 ml</LI></UL> | distilled water to 500 ml<BR></LI></UL> | ||
VP Reagent A <UL><LI> | VP Reagent A <UL><LI> | ||
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VP Reagent B<UL><LI> | VP Reagent B<UL><LI> | ||
40g potassium hydroxide<BR> | 40g potassium hydroxide<BR> | ||
bring to volume with 100 ml distilled water</LI></UL> | bring to volume with 100 ml distilled water</LI></UL><BR> | ||
'''SIM agar and Reagent recipes:'''<UL><LI> | '''SIM agar and Reagent recipes:'''<UL><LI>< | ||
Approximate Formula* Per Liter<BR> | Approximate Formula* Per Liter<BR> | ||
Revision as of 12:36, 18 January 2010
Misc Recipes for test media and their reagents
Lab 4
-
10g tryptose
3g Beef extract
5g Sodium Chloride
2.5g Phenylethyl alcohol
15g Agar to 1 liter distilled or deionized water
pH 7.1-7.5.
-
10g peptone
10g Lactose
2g dipotassium phosphate
0.4g eosin Y
0.065 g methylene blue
15 g Agar
final pH 6.9-7.3
Lab 5
catalase test
fresh hydrogen peroxide
Oxidase test
order fresh commercial kits
positive controls: Bacillus spp, Neisseria, Micrococcus or Pseudomonas
OF-glucose medium
0.2 per cent peptone
0.5 per cent NaCl
0.03 per cent K2HPO4
0.3 per cent agar
0.003 per cent bromthymol blue
1.0 per cent carbohydrate
pH 7.1.
cellulose degradation medium
(use melted agar of the appropiate enrichment media choices
or try Nutrient agar for all???
Ammonification reagent
Nesler's reagent will be purchased commercially. Please be extremely careful handling this solution.
-
Recipe: 0.09 mol/L solution of potassium tetraiodomercurate(II) (K2[HgI4]) in 2.5 mol/L potassium hydroxide. A yellow coloration indicates the presence of ammonia: at higher concentrations, a brown precipitate may form. The sensitivity as a spot test is about 0.3 μg NH3 in 2 μL.
NH4+ + 2[HgI4]2− + 4OH− → HgO·Hg(NH2)I + 7I− + 3H2O
It is toxic if swallowed, inhaled or absorbed through the skin. It presents a neurological hazard and may act as a carcinogen and be a reproductive hazard. It is corrosive and causes burns. Wear gloves and consider performing this assay in a hood.
Lab 6
-
dextrose peptone broth
0.7% peptone,
0.5% dextrose
0.5% dipotassium phosphate
pH 6.7-7.1
-
0.1 methyl red dye
ethanol 00 ml
distilled water to 500 ml
-
5 g alpha naphthol
bring volume to 100 ml absolute alcohol
-
40g potassium hydroxide
bring to volume with 100 ml distilled water
- <
Approximate Formula* Per Liter
Pancreatic Digest of Casein - 20.0 g
Peptic Digest of Animal Tissue - 6.1 g
Ferrous Ammonium Sulfate - 0.2 g
Sodium Thiosulfate - 0.2 g
Agar - 3.5 g -
Kovac's reagent: (per liter)
p-Dimethylaminobenzaldehyde 50,0g
Amyl Alcohol 750.0 ml
Hydrochloric acid 250.0 ml -
Indole Spot Test Reagent
p-Dimethylaminocinnamaldehyde 10.0 g
Hydrochloric acid 100.0ml
Water, deionized 900.0 ml
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
