BISC209: Recipes

Misc Recipes for test media and their reagents

SIM agar and Reagent recipes:

• SIM agar:
• Approximate Formula* Per Liter
  Pancreatic Digest of Casein - 20.0 g
  Peptic Digest of Animal Tissue - 6.1 g
  Ferrous Ammonium Sulfate - 0.2 g
  Sodium Thiosulfate - 0.2 g
  Agar - 3.5 g
• Kovac's reagent: (per liter)
  p-Dimethylaminobenzaldehyde 50,0g
  Amyl Alcohol 750.0 ml
  Hydrochloric acid 250.0 ml
• Indole Spot Test Reagent
  p-Dimethylaminocinnamaldehyde 10.0 g
  Hydrochloric acid 100.0ml
  Water, deionized 900.0 ml