BISC209: Recipes

Misc Recipes for test media and their reagents

Lab 4:

PEA MEDIUM

• 10g tryptose
  3g Beef extract
  5g Sodium Chloride
  2.5g Phenylethyl alcohol
  15g Agar to 1 liter distilled or deionized water
  pH 7.1-7.5.

EMB medium

• 10g peptone
  10g Lactose
  2g dipotassium phosphate
  0.4g eosin Y
  0.065 g methylene blue
  15 g Agar
  final pH 6.9-7.3
  
  

Lab 5 catalase test
fresh hydrogen peroxide<BR
Oxidase test
order fresh commercial kits
postitive controls: Bacillus spp, Neisseria, Micrococcus or Pseudomonas)

Lab 6

MRVPMethyl red test

• dextrose peptone broth
  0.7% peptone,
  0.5% dextrose
  0.5% dipotassium phosphate
  pH 6.7-7.1

Methyl red Reagent

• 0.1 methyl red dye
  ethanol 00 ml
  distilled water to 500 ml

VP Reagent A

• 5 g alpha naphthol
  bring volume to 100 ml absolute alcohol

VP Reagent B

• 40g potassium hydroxide
  bring to volume with 100 ml distilled water

SIM agar and Reagent recipes:

• Approximate Formula* Per Liter
  Pancreatic Digest of Casein - 20.0 g
  Peptic Digest of Animal Tissue - 6.1 g
  Ferrous Ammonium Sulfate - 0.2 g
  Sodium Thiosulfate - 0.2 g
  Agar - 3.5 g
• Kovac's reagent: (per liter)
  p-Dimethylaminobenzaldehyde 50,0g
  Amyl Alcohol 750.0 ml
  Hydrochloric acid 250.0 ml
• Indole Spot Test Reagent
  p-Dimethylaminocinnamaldehyde 10.0 g
  Hydrochloric acid 100.0ml
  Water, deionized 900.0 ml
  

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12