BISC209: Recipes
From OpenWetWare
Misc Recipes for test media and their reagents
Lab 4:
PEA MEDIUM-
10g tryptose
3g Beef extract
5g Sodium Chloride
2.5g Phenylethyl alcohol
15g Agar to 1 liter distilled or deionized water
pH 7.1-7.5.
-
10g peptone
10g Lactose
2g dipotassium phosphate
0.4g eosin Y
0.065 g methylene blue
15 g Agar
final pH 6.9-7.3
Lab 5
catalase test
fresh hydrogen peroxide<BR
Oxidase test
order fresh commercial kits
postitive controls: Bacillus spp, Neisseria, Micrococcus or Pseudomonas)
OF-glucose medium
0.2 per cent peptone
0.5 per cent NaCl
0.03 per cent K2HPO4
0.3 per cent agar
0.003 per cent bromthymol blue
1.0 per cent carbohydrate
pH 7.1.
cellulose degradation medium:
(use melted agar of the appropiate enrichment media choices
or try Nutrient agar for all???
Lab 6
-
dextrose peptone broth
0.7% peptone,
0.5% dextrose
0.5% dipotassium phosphate
pH 6.7-7.1
-
0.1 methyl red dye
ethanol 00 ml
distilled water to 500 ml
-
5 g alpha naphthol
bring volume to 100 ml absolute alcohol
-
40g potassium hydroxide
bring to volume with 100 ml distilled water
-
Approximate Formula* Per Liter
Pancreatic Digest of Casein - 20.0 g
Peptic Digest of Animal Tissue - 6.1 g
Ferrous Ammonium Sulfate - 0.2 g
Sodium Thiosulfate - 0.2 g
Agar - 3.5 g -
Kovac's reagent: (per liter)
p-Dimethylaminobenzaldehyde 50,0g
Amyl Alcohol 750.0 ml
Hydrochloric acid 250.0 ml -
Indole Spot Test Reagent
p-Dimethylaminocinnamaldehyde 10.0 g
Hydrochloric acid 100.0ml
Water, deionized 900.0 ml
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12