BISC209: Stock cultures: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 35: | Line 35: | ||
| colorless colonies | | colorless colonies | ||
|- | |- | ||
<BR> | |||
Lab 5<BR> | |||
catalase test<UL><LI> | |||
positive control: <BR> | |||
Bacillus spp or Staph. epi<BR><BR> | |||
Negative control:<BR> | |||
Enterococcus faecalis<BR> | |||
</LI></UL> | |||
oxidase test<UL><LI> | |||
Escherichia coli</LI></UL> | |||
==Links to Labs== | ==Links to Labs== |
Revision as of 11:17, 18 January 2010
Stock Cultures
Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)
Lab 4:
Gram pos: PEA growth<BR
Staph epi.
Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.
Lab 5
catalase test
-
positive control:
Bacillus spp or Staph. epi
Negative control:
Enterococcus faecalis
- Escherichia coli
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
Organism | Colonial Appearance |
---|---|
Escherichia coli | purple with black center/ green metallic sheen |
Klebsiella pneumoniae | dark centered colonies/ green metallic sheen |
Enterobacter aeorogenes | pink colonies/ no metallic sheen |
Proteus mirabilis | colorless colonies |
Salmonella typhimurium | colorless colonies |