BISC209: Stock cultures: Difference between revisions

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==Stock Cultures==
'''Lab 1:'''<BR>
''Staphylococcus epidermidis''<BR>
''Serratia marcescens'' maintained at both 27 (red) and 37 (white)<BR>


'''Lab 4:''' <BR>
Gram pos: PEA growth<BR>
''Staph epi.''<BR><BR>


Gram neg EMB growth <BR>
any of these:<BR>
'''Table 2.''' Colonial appearance on EMB Agar after 18-24 hours at 35°C.  A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.<BR>


{| border="1"
|+
! '''Organism''' !! '''Colonial Appearance'''
|-
! ''Escherichia coli''
| purple with black center/ green metallic sheen
|-
! ''Klebsiella pneumoniae''
| dark centered colonies/ green metallic sheen
|-
! ''Enterobacter aeorogenes''
| pink colonies/ no metallic sheen
|-
! ''Proteus mirabilis''
| colorless colonies
|-
! ''Salmonella typhimurium''
| colorless colonies
|-
|}
<BR><BR>


'''Lab 5'''<BR>
catalase test<UL><LI>
positive control: <BR>
''Bacillus'' or ''Staph. epi''<BR><BR>


Negative control:<BR>
''Enterococcus faecalis''<BR>
</LI></UL>
oxidase test<UL><LI>
''Escherichia coli''</LI></UL>


'''Stock Cultures'''
Antibiotic plates control orgs (2 Gram pos 1 Gram neg):<UL><LI>
Lab 1:<BR>
''Eschericia coli'' (Gram negative), ''Staphylococcus epidermidis'' (Gram positive) and ''Micrococcus luteus''<BR><BR>
Staphylococcus epidermidis<BR>
Serratia marcescens maintained at both 27 (red) and 37 (white)<BR>
 
Lab 4: <BR>
Gram neg EMB growth
E. coli
Gram pos: PEA growth
Staph epi.
 
 
 
 
 
 
 
 
 


Starch plate <UL><LI>
pos. controls ''Bacillus subtilis'' <BR>
neg control: ''Staph. sp.''?<BR>
</LI></UL>
'''Lab 6 '''<BR>
MRVP TEST <UL><LI>
MR pos control:  ''Escherichia coli''<BR>
VP pos control:  ''Enterobacter aerogenes''
</LI></UL>


==Links to Labs==
==Links to Labs==

Latest revision as of 19:16, 19 January 2010

Wellesley College-BISC 209 Microbiology -Spring 2010

Stock Cultures

Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)

Lab 4:
Gram pos: PEA growth
Staph epi.

Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.

Organism Colonial Appearance
Escherichia coli purple with black center/ green metallic sheen
Klebsiella pneumoniae dark centered colonies/ green metallic sheen
Enterobacter aeorogenes pink colonies/ no metallic sheen
Proteus mirabilis colorless colonies
Salmonella typhimurium colorless colonies



Lab 5

catalase test
  • positive control:
    Bacillus or Staph. epi

    Negative control:
    Enterococcus faecalis
oxidase test
  • Escherichia coli
Antibiotic plates control orgs (2 Gram pos 1 Gram neg):
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