BISC209: Stock cultures: Difference between revisions
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==Stock Cultures== | ==Stock Cultures== | ||
Lab 1:<BR> | '''Lab 1:'''<BR> | ||
Staphylococcus epidermidis<BR> | ''Staphylococcus epidermidis''<BR> | ||
Serratia marcescens maintained at both 27 (red) and 37 (white)<BR> | ''Serratia marcescens'' maintained at both 27 (red) and 37 (white)<BR> | ||
Lab 4: <BR> | '''Lab 4:''' <BR> | ||
Gram pos: PEA growth<BR | Gram pos: PEA growth<BR> | ||
Staph epi.<BR><BR> | ''Staph epi.''<BR><BR> | ||
Gram neg EMB growth <BR> | Gram neg EMB growth <BR> | ||
| Line 35: | Line 35: | ||
| colorless colonies | | colorless colonies | ||
|- | |- | ||
<BR> | |} | ||
Lab 5<BR> | <BR><BR> | ||
'''Lab 5'''<BR> | |||
catalase test<UL><LI> | catalase test<UL><LI> | ||
positive control: <BR> | positive control: <BR> | ||
Bacillus | ''Bacillus'' or ''Staph. epi''<BR><BR> | ||
Negative control:<BR> | Negative control:<BR> | ||
Enterococcus faecalis<BR> | ''Enterococcus faecalis''<BR> | ||
</LI></UL> | </LI></UL> | ||
oxidase test<UL><LI> | oxidase test<UL><LI> | ||
Escherichia coli</LI></UL> | ''Escherichia coli''</LI></UL> | ||
Antibiotic plates control orgs (2 Gram pos 1 Gram neg):<UL><LI> | |||
''Eschericia coli'' (Gram negative), ''Staphylococcus epidermidis'' (Gram positive) and ''Micrococcus luteus''<BR><BR> | |||
Lab 6 <BR> | Starch plate <UL><LI> | ||
MR pos control: Escherichia coli<BR> | pos. controls ''Bacillus subtilis'' <BR> | ||
VP pos | neg control: ''Staph. sp.''?<BR> | ||
</LI></UL> | |||
'''Lab 6 '''<BR> | |||
MRVP TEST <UL><LI> | |||
MR pos control: ''Escherichia coli''<BR> | |||
VP pos control: ''Enterobacter aerogenes'' | |||
</LI></UL> | |||
==Links to Labs== | ==Links to Labs== | ||
Latest revision as of 19:16, 19 January 2010
Stock Cultures
Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)
Lab 4:
Gram pos: PEA growth
Staph epi.
Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.
| Organism | Colonial Appearance |
|---|---|
| Escherichia coli | purple with black center/ green metallic sheen |
| Klebsiella pneumoniae | dark centered colonies/ green metallic sheen |
| Enterobacter aeorogenes | pink colonies/ no metallic sheen |
| Proteus mirabilis | colorless colonies |
| Salmonella typhimurium | colorless colonies |
Lab 5
-
positive control:
Bacillus or Staph. epi
Negative control:
Enterococcus faecalis
- Escherichia coli
-
Eschericia coli (Gram negative), Staphylococcus epidermidis (Gram positive) and Micrococcus luteus
Starch plate-
pos. controls Bacillus subtilis
neg control: Staph. sp.?
Lab 6
MRVP TEST
-
MR pos control: Escherichia coli
VP pos control: Enterobacter aerogenes
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
-
pos. controls Bacillus subtilis
