BISC219/F11:Calendars/Planner: Difference between revisions

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|  Sept. 5 <br>'''Labor Day '''<br> '''No Class'''
|  Sept. 5 <br>'''Labor Day '''<br> '''No Class'''
| Sept. 6 <br> Lab 1 - Part 2 || Sept. 7  <br> '''Mon. Schedule''' <br> Lab 1 || Sept. 8  <br> Lab 2 || Sept. 9 <br> Lab 2 ||  
| Sept. 6 <br> Lab 1 - Part 2 || Sept. 7  <br> '''Mon. Schedule''' <br> Lab 1<br>Wed. lab meets 6-7pm <br>for scoring || Sept. 8  <br> Lab 2 || Sept. 9 <br> Lab 2 ||  
|-
|-
| Sept. 12 <br>Lab 2  
| Sept. 12 <br>Lab 2  
| Sept. 13 <br>Lab 2 || Sept. 14 <br>Lab 2 || Sept. 15 <br>Lab 3 || Sept. 16 <br>Lab 3 ||   
| Sept. 13 <br>Lab 2 || Sept. 14 <br>Lab 2<BR> Mon. lab meets 6-7pm<BR> for scoring || Sept. 15 <br>Lab 3 || Sept. 16 <br>Lab 3 ||   
|-
|-
| Sept. 19 <br>Lab 3
| Sept. 19 <br>Lab 3
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| Oct. 24 <br>Lab 7
| Oct. 24 <br>Lab 7
| Oct. 24 <br>Lab 7 || Oct. 26 <BR> '''No Lab''' ||  Oct. 27 <br> '''No Lab'''|| Oct. 28 <br> '''No Lab''' ||   
| Oct. 25 <br>Lab 7 || Oct. 26 <BR> '''No Lab''' ||  Oct. 27 <br> '''No Lab'''|| Oct. 28 <br> '''No Lab''' ||   
|-
|-
| Oct. 31 <br> '''No Lab'''
| Oct. 31 <br> '''No Lab'''
| Nov. 1 <br> Tanner Conference <br> '''No Lab''' || Nov. 2  <br> Lab 8 ||  Nov. 3 <br> Lab 8 || Nov. 4 <br> Lab 8||   
| Nov. 1 <br> '''Tanner Conference''' <br> '''No Lab''' || Nov. 2  <br> Lab 8 ||  Nov. 3 <br> Lab 8 || Nov. 4 <br> Lab 8<BR>''' Paper 2 due<BR> for all students'''||   
|-
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| Nov. 7 <br> Lab 8
| Nov. 7 <br> Lab 8
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|-
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| Dec. 5 <br> '''No Lab'''
| Dec. 5 <br> '''No Lab'''
| Dec. 6 <br> '''No Lab''' || Dec. 7  <br> '''No Lab''' <br> '''Last Day of Class''' ||  Dec. 8 <br>|| Dec. 9 <br> ||   
| Dec. 6 <br> '''No Lab''' || Dec. 7  <br> '''No Lab''' <br> '''Last Day of Class'''<BR>'''Paper 3 due <BR>for all students''' ||  Dec. 8 <br>|| Dec. 9 <br> ||   
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|-
|-
!3  
!3  
| Reverse Genetics: Gene selection, Cloning & Making a feeding vector, RNAi, RT-PCR
| Reverse Genetics: Gene selection, Cloning & Making a feeding vector, RNAi
| 5-12
| 5-11
|-
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|}
|}
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|-
|-
! 1
! 1
| Sept. 7 to <br>Sept. 13
| Aug 30 to <br> Sept. 7
| '''LEARN WORM HUSBANDRY;''' <BR>View worm videos;<BR> Make your worm pick; <BR>Examine worms: recognize different stages & sexes & differentiate mutations;<BR> Practice picking worms;<BR>'''Start Series 1: Set up autosomal and X-linked crosses'''
| '''LEARN WORM HUSBANDRY;''' <BR>View worm videos;<BR> Make your worm pick; <BR>Examine worms: recognize different stages & sexes <BR> Practice picking worms;<BR>'''Start Series 1: Set up autosomal and X-linked crosses'''
|'''3 days after lab:'''  pick (2) wild type worms from each cross to new plates (3 plates total);<br>Examine the phenotypes of the progeny – hermaphrodites and males - record all information in  your notebook
|'''3 days after lab:'''  pick (2) wild type worms from each cross to new plates (3 plates total);<br>Examine the phenotypes of the progeny – hermaphrodites and males - record all information in  your notebook
| '''Homework''':Complete Entry Survey; Familiarize yourself with the information in the [[BISC_219/F10:Resources]] section;<br>Read all of the background information on ''C. elegans'' found in [[BISC_219/F10:_Worm_Info]] and read the Lab 1 and Lab 2 information about our first Series 1: Autosomal vs. Sex-Linked Inheritance at [[BISC_219/F10:_Gene_Linkage]] & [[BISC_219/F10:_Lab_2]]; Complete Graded Assignment 1 explained at [[BISC_219/F10: Assignment_1_Lab1]]. Download and use for your cross diagrams: [[Media:Template_for_Crosses.ppt]]
| '''Homework''': Complete Entry Survey; Familiarize yourself with the information in the [[BISC219/F11:Resources]] section;<br>Read all of the background information on ''C. elegans'' found in [[BISC219/F11:_Worm_Info]] and read the Lab 1 and Lab 2 information about our first Series 1: Autosomal vs. Sex-Linked Inheritance at [[BISC219/F11:_Gene_Linkage]] & [[BISC219/F11:_Lab_2]]; Complete Graded Assignment 1 explained at [[BISC219/F11: Assignment_1_Lab1]]
|-
|-
! 2
! 2
| Sept. 14 to <br> Sept 20
| Sept. 8 to <br> Sept 14
| '''Complete Series 1:''' Count and examine phenotypes of autosomal vs. X-linked crosses; <BR> '''Start Series 2: Classical (Forward) Genetics- Part 1- Perform mutant hunt:''' <BR> Pick (3) putative Dpy mutants to separate plates
| '''Complete Series 1:''' Count and examine phenotypes of autosomal vs. X-linked crosses; <BR> '''Start Series 2: Classical (Forward) Genetics- Part 1- Examine mutant worms and compare to wild type:''' <BR> Pick (3) putative Dpy mutants to separate plates
|'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate)
|'''3 days after lab''':Examine mutants: check phenotype <BR> if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate)
| '''Homework:''' Read ALL of Series 2:background information [[BISC_219/F10:_Gene_Mapping_Info]] and about all work to be performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our overall topic and experimental question(s) & goals. Outline the experimental process. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species.  Assignment explained and rubric found at [[BISC_219/F10: Assignment Series2_Outline_Summary]]<BR>
| '''Homework:''' Read ALL of Series 2:background information [[BISC219/F11:_Gene_Mapping_Info]] and about all work to be performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our overall topic and experimental question(s) & goals. Outline the experimental process. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species.  Assignment explained and rubric found at [[BISC219/F11: Assignment Series2_Outline_Summary]]<BR>
Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]]; <BR> Read the journal article, Indentification of Genes that Regulate a Left-Right Aymmetric Neuronal Migration in ''Caenorhabditis elegans'' published in ''Genetics'' 164: 1355-1367 (August 2003), for discussion in lab next time. Full text of article found at: [http://www.genetics.org/cgi/content/full/164/4/1355]; See [[BISC_219/F10:Questions to Guide Your Reading1]] for information on how to prepare for this discussion
Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC219/F11: Assignment Help- Data Analysis 1]]
|-
|-
! 3
! 3
| Sept. 21 to <br> Sept. 27
| Sept. 15 to <br> Sept. 21
| '''Series 2- Part 2: Linkage Analysis''': cross heterozygous males (+/d) from last cross to the (5) test strains (5 plates total);<BR> Journal article discussion
| '''Series 2- Part 2: Linkage Analysis''': cross heterozygous males (+/d) from last cross to the (5) test strains (5 plates total);<BR>
| '''3 days after lab: Linkage:''' transfer (2) L4 hermaphrodites from each cross<br> to new plates (5 plates total)
| '''3 days after lab: Linkage:''' transfer (2) L4 hermaphrodites from each cross<br> to new plates (5 plates total)
| '''Homework: '''Draw crosses and diagram strategy for Series 2 Linkage Analysis due at the beginning of Lab 4. Information and grading rubric found at [[BISC_219/F10: Assignment_Series2_Linkage Testing Crosses]]. Template for crosses for this homework and next week's downloadable at:[[Media:Series2_Template_for_Crosses.pptx]]<BR>
| '''Homework: '''Draw crosses and diagram strategy for Series 2 Linkage Analysis due at the beginning of Lab 4. Information and grading rubric found at [[BISC219/F11: Assignment_Series2_Linkage Testing Crosses]].  
Con't Data Analysis (Results)of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]]
Con't Data Analysis (Results)of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC219/F11: Assignment Help- Data Analysis 1]]
|-
|-
! 4
! 4
| Sept. 28 to <br> Oct. 4
| Sept. 22 to <br> Sept. 28
| '''Complete Linkage Analysis:''' examine phenotypes and count to determine linkage;<BR> '''Part 3: Start Mapping:''' pick (6) Uncs of the linked unc  strain to separate plates (6 plates total);<BR> '''Part 4: Start Complementation Analysis:''' Cross Dpy mutant worms to N2 males (2 plates total)
| '''Complete Linkage Analysis:''' examine phenotypes and count to determine linkage;<BR> '''Part 3: Start Mapping:''' pick (6) Uncs of the linked unc  strain to separate plates (6 plates total);<BR> '''Part 4: Start Complementation Analysis:''' Cross Dpy mutant worms to N2 males (2 plates total)
| '''3 days after lab: Mapping:''' <BR>Pick (3) double mutants to separate plates (3 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR>
| '''3 days after lab: Mapping:''' <BR>Pick (3) double mutants to separate plates (3 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR>
| '''Homework:''' Draw crosses and diagram strategy for Mapping the Location of your ''dpy'' mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 5. Assignment described at [[BISC_219/F10: Assignment_Series2_Mapping Crosses]]; Read the journal article by Davis ''at al.'',Rapid single nucleotide polymorphism mapping in C. elegans, found at BMC Genomics 2005, 6:118 [http://dx.doi.org/10.1186/1471-2164-6-118 doi:10.1186/1471-2164-6-118] and compare their mapping strategy to yours.
| '''Homework:''' Explain complementation analysis in general and specifically how it was used to id your ''dpy'' gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses using template provided. Due at the beginning of Lab 5. Assignment described at[[BISC219/F11: Assignment_Series2_Complementation]]  
|-
|-
! 5
! 5
| Oct. 5 to<br> Oct. 12
| Sept. 29 to<br> Oct. 5
| '''Series2:Mapping:'''Self double mutants to keep viable true-breeding progeny;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene
| '''Series2: Mapping:'''Self double mutants to keep viable true-breeding progeny;<BR>'''Series2: Complementation:''' examine cross plates for Dpy males - WHY?; '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis  
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis  
| '''Homework:''' Explain complementation analysis in general and specifically how it was used to id your ''dpy'' gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]]
| '''Homework:''' Draw crosses and diagram strategy for Mapping the Location of your ''dpy'' mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 6. Assignment described at [[BISC219/F11: Assignment_Series2_Mapping Crosses]]
|-
|-
! 6
! 6
| Oct. 13 to <br> Oct. 19
| Oct. 12 to <br> Oct. 18
|  '''Series 2: Mapping''': cross N2 males (++/++) with L4 double mutant hermaphrodites (2 plates total);Self double mutants to new plate<BR> Series2: Characterizing the dpy mutation''' Gene Sequence Analysis <br> '''Series3: Reverse Genetics:'''Restriction enzyme digest PCR product/clean-up/ligation into pPD129.36 vector/transformation into BL21 ''E.coli''
|  '''Series 2: Mapping''': cross N2 males (++/++) with L4 double mutant hermaphrodites (2 plates total);Self double mutants to new plate<BR> Series2: Characterizing the dpy mutation''' Gene Sequence Analysis <br> '''Series3: Reverse Genetics:'''Restriction enzyme digest PCR product/clean-up/ligation into pPD129.36 vector/transformation into BL21 ''E.coli''
|  '''3 days after lab:Series2: Mapping:''' pick (4) male heterozygotes (++/d u) and Cross them with L4 hermaphrodite double mutants (d u/d u). <BR>'''Series 3:'''Check control & transformation plates (save) – <BR>notify instructor if no colonies
|  '''3 days after lab:Series2: Mapping:''' pick (4) male heterozygotes (++/d u) and Cross them with L4 hermaphrodite double mutants (d u/d u). <BR>'''Series 3:'''Check control & transformation plates (save) – <BR>notify instructor if no colonies
| '''Homework''': DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at [[BISC_219/F10: Assignment_Series2_DNA Sequencing]]<BR>
| '''Homework''': DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at [[BISC219/F11: Assignment_Series2_DNA Sequencing]]<BR>
Scientific research report on Series2 Classical (Forward) Genetics Project due week of 11/1 at the beginning of lab. 5%/day late penalty! See assignment directions at: [[BISC 219/F10: Assignment_ Series2_Classical Genetics Paper]]
|-
|-
! 7
! 7
| Oct. 20 to <br> Oct. 26
| Oct. 19 to <br> Oct. 25
| '''Series2:Finish Mapping:''' SCORE! Calculate Recombination frequency; <BR>'''Series3:Reverse Genetics''' Colony PCR to look for bacterial transformants
| '''Series2:Finish Mapping:''' SCORE! Calculate Recombination frequency; <BR>'''Series3:Reverse Genetics''' Colony PCR to look for bacterial transformants
| Determine map distance between your mutant gene <BR>and the known reference mutation<BR>'''Day before lab:''' Series 3:grow overnight culture of positive colony
| Determine map distance between your mutant gene <BR>and the known reference mutation<BR>'''Day before lab:''' Series 3:grow overnight culture of positive colony
| '''Homework''': Work on your Series 2: Forward genetics paper due week of Nov 1
| '''Homework''': Scientific research report on Series 2 Classical (Forward) Genetics Project due Fri. Nov. 4 for ALL students. 5%/day late penalty! See assignment directions at: [[BISC219/F11: Assignment_ Series2_Classical Genetics Paper]]
|-
|-
! 8
! 8
| Oct. 28 to <br> Nov. 3
| Nov. 2 to <br> Nov. 8
| '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells
| '''Series3: Reverse Genetics''': Plasmid isolation/quantification of DNA/ transformation of plasmid into ''E. coli'' strain HT115(DE3)
| '''Day before lab:''' grow overnight culture of single colony from transformation
| '''Day before lab:''' grow overnight culture of single colony from transformation
| '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]]. Due at the beginning of Lab 9 ; Read the original 1998 paper by Fire and Mello published in ''Nature'' '''391''': 806-811 (Feb.19 1998) at [http://dx.doi.org/10.1038/35888 doi:10.1038/35888] ; Do your own library search to find and read a recent review article about RNAi in ''C. elegans''. Also read the 2002 Simmer ''et al.'' paper in ''Current Biology'' '''12''':1317-1319 explaining the ''rrf-3'' strain that we will use for our RNAi work at [http://dx.doi.org/10.1016/S0960-9822(02)01041-2 doi:10.1016/S0960-9822(02)01041-2 ]. These articles provide background information on how the mechanism of RNAi was worked out experimentally and will be helpful to you when you write the introduction section of your Series3 paper. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A ''Caenorhabditis elegans'' Model. PLoS ONE 4(8): e6860, found at [http://dx.doi.org/10.1371/journal.pone.0006860 doi:10.1371/journal.pone.0006860]. This study, like yours, uses reverse genetics to investigate gene function. Be prepared to discuss this paper in Lab 9.  
| '''Homework''': Write all Series 3 Protocols as Materials & Methods. Assignment described at [[BISC219/F11: Assignment_Series3_ Materials and Methods]]. Due at the beginning of Lab 9.


|-
|-
! 9
! 9
| Nov.4 to <br> Nov. 10
| Nov. 9 to <br> Nov. 15
| '''Series3:RNAi''' : Induction of bacteria and seed plates for RNAi feeding; <BR> Journal article discussion
| '''Series3:RNAi''' : Induction of bacteria and seed plates for RNAi feeding; <BR> Journal article discussion
|  '''4 days later:''' Pick2 L4 hermaphrodite worms of N2 and ''rrf-3''genotype to 2 RNAi plates for each genotype and make 1 control 'mock"plate for each genotype (6plates);  
|  '''4 days later:''' Pick 2 L4 hermaphrodite worms of N2 and ''rrf-3'' genotype to 2 RNAi plates for each genotype and make 1 control ''mock'' plate for each genotype (6 plates);  
| '''Homework:'''Write a draft introduction section (including properly formatted Literature Cited page) of your next paper on our Reverse Genetics Project. Due at the beginning of Lab 10. Refer to [[BISC_219/F10:Resources]] Guide to Scientific Writing.  
| '''Homework:'''Write a draft introduction section (including properly formatted Literature Cited page) of your next paper on our Reverse Genetics Project. Due at the beginning of Lab 10. Refer to [[BISC219/F11:Resources]] Guide to Scientific Writing.  
|-
|-
! 10
! 10
| Nov. 11 to <br> Nov. 17
| Nov. 16 to <br> Nov. 22
| '''Series3:RNAi:''' SCORING (collection)of phenotype of fed worms compared to control N2 worms & womrs with gene mutation; Collect treated worms for RNA purification
| '''Series3:RNAi:''' SCORING (collection)of phenotype of fed worms compared to control N2 worms & worms with gene mutation
| _
| --
| '''Homework''': Construct figures/tables with properly formatted legends to illustrate the main findings of the RNAi part of your Series3:Reverse Genetics project. These figures are a draft of those that will be part of the results section of the research report you will write for Series3. Due on Nov. 24 for all students.
| '''Homework''': Construct figures/tables with properly formatted legends to illustrate the main findings of the RNAi part of your Series3:Reverse Genetics project. These figures are to be used in a Science Writing workshop in Lab 11. Full paper on Series 3 Due last day of classes for all students. Read the [http://dx.doi.org/10.1038/35888 original 1998 paper by Fire and Mello] published in ''Nature'' '''391''': 806-811 (Feb.19 1998); Do your own library search to find and read a recent review article about RNAi in ''C. elegans''. Also read the 2002 Simmer ''et al.'' paper in ''Current Biology'' '''12''':1317-1319 [http://dx.doi.org/10.1016/S0960-9822(02)01041-2 explaining the ''rrf-3'' strain] that we will use for our RNAi work. These articles provide background information on how the mechanism of RNAi was worked out experimentally and will be helpful to you when you write the introduction section of your Series3 paper. Also read a published journal article by Green et al. (2009) [http://dx.doi.org/10.1371/journal.pone.0006860 Impact of Cigarette Smoke Exposure on Innate Immunity: A ''Caenorhabditis elegans'' Model] PLoS ONE 4(8): e6860. This study, like yours, uses reverse genetics to investigate gene function.  
|-
|-
! NO LAB
! NO LAB
| Nov. 18 to <br> Nov. 26
| Nov. 23 to <br> Nov. 25
| Thanksgiving Break
| Thanksgiving Break
| _
| _
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|-
! 11
! 11
| Nov. 29 to<br> Dec. 3
| Nov. 28 to<br> Dec. 2
| '''Series 3:Reverse Genetics''' RT PCR;  
| '''Series 3:Reverse Genetics''' Science Writing Workshop & Effective Figure Design. Be sure to have read the [http://dx.doi.org/10.1038/35888 original 1998 paper by Fire and Mello] published in ''Nature'' '''391''': 806-811 (Feb.19 1998); Do your own library search to find and read a recent review article about RNAi in ''C. elegans''. Also read the 2002 Simmer ''et al.'' paper in ''Current Biology'' '''12''':1317-1319 [http://dx.doi.org/10.1016/S0960-9822(02)01041-2 explaining the ''rrf-3'' strain] that we will use for our RNAi work. These articles provide background information on how the mechanism of RNAi was worked out experimentally and will be helpful to you when you write the introduction section of your Series3 paper. Also read a published journal article by Green et al. (2009) [http://dx.doi.org/10.1371/journal.pone.0006860 Impact of Cigarette Smoke Exposure on Innate Immunity: A ''Caenorhabditis elegans'' Model] PLoS ONE 4(8): e6860. This study, like yours, uses reverse genetics to investigate gene function.
| Instructor will run gel; when results available review and interpret gel image
| --
| '''Homework''': Full Scientific Research Report on Reverse Genetics Project – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References See [[BISC_219/F10:Resources]] section '''Guide to Scientific Wriing''' and [[BISC_219/F10: Assignment_Series3_Reverse Genetics Paper using RNAi]]  
| '''Homework''': Full Scientific Research Report on Reverse Genetics Project – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References See [[BISC219/F11:Resources]] section '''Guide to Scientific Wriing''' and [[BISC219/F11: Assignment_Series3_Reverse Genetics Paper using RNAi]].  
|-
DUE Dec. 7 by 5 pm for ALL students. Complete exit genetic assessment. See Sakai site for link and for how to obtain your incentive points.  
! 12
| Dec. 6 to <br> Dec. 10
| TBA
| TBA
| Scientific Research Report on Reverse Genetics project DUE Dec. 10 (last day of classes) by 4pm for ALL students Assignment information at [[BISC_219/F10: Assignment_Series3_Reverse Genetics Paper using RNAi]]; Complete exit genetic assessment. See First Class conference for link and for how to obtain your incentive points (2.5).  
|-
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Latest revision as of 05:29, 29 September 2011

Wellesley College BISC 219 Genetics

BISC219 F11 Lab Calendar


Monday Tuesday Wednesday Thursday Friday
Aug. 29
No Class 		Aug. 30
Lab 1 - Part 1		 Aug. 31
Lab 1		 Sept. 1
Lab 1		 Sept. 2
Lab 1
Sept. 5
Labor Day
No Class 		Sept. 6
Lab 1 - Part 2		 Sept. 7
Mon. Schedule
Lab 1
Wed. lab meets 6-7pm
for scoring		 Sept. 8
Lab 2		 Sept. 9
Lab 2
Sept. 12
Lab 2 		Sept. 13
Lab 2		 Sept. 14
Lab 2
Mon. lab meets 6-7pm
for scoring		 Sept. 15
Lab 3		 Sept. 16
Lab 3
Sept. 19
Lab 3 		Sept. 20
Lab 3		 Sept. 21
Lab 3		 Sept. 22
Lab 4		 Sept. 23
Lab 4
Sept. 26
Lab 4 		Sept. 27
Lab 4		 Sept. 28
Lab 4		 Sept. 29
Lab 5		 Sept. 30
Lab 5
Oct. 3
Lab 5 		Oct. 4
Lab 5		 Oct. 5
Lab 5		 Oct. 6
No Lab		 Oct. 7
No Lab
Oct. 10
Fall Break
No Lab 		Oct. 11
Fall Break
No Lab		 Oct. 12
Lab 6		 Oct. 13
Lab 6		 Oct. 14
Lab 6
Oct. 17
Lab 6 		Oct. 18
Lab 6		 Oct. 19
Lab 7		 Oct. 20
Lab 7		 Oct. 21
Lab 7
Oct. 24
Lab 7 		Oct. 25
Lab 7		 Oct. 26
No Lab		 Oct. 27
No Lab		 Oct. 28
No Lab
Oct. 31
No Lab 		Nov. 1
Tanner Conference
No Lab		 Nov. 2
Lab 8		 Nov. 3
Lab 8		 Nov. 4
Lab 8
Paper 2 due
for all students
Nov. 7
Lab 8 		Nov. 8
Lab 8		 Nov. 9
Lab 9		 Nov. 10
Lab 9		 Nov. 11
Lab 9
Nov. 14
Lab 9 		Nov. 15
Lab 9		 Nov. 16
Lab 10		 Nov. 17
Lab 10		 Nov. 18
Lab 10
Nov. 21
Lab 10 		Nov. 22
Lab 10		 Nov. 23
No Lab		 Nov. 24
Thanksgiving Holiday		 Nov. 25
Thanksgiving Holiday
Nov. 28
Lab 11 		Nov. 29
Lab 11		 Nov. 30
Lab 11		 Dec. 1
Lab 11		 Dec. 2
Lab 11
Dec. 5
No Lab 		Dec. 6
No Lab		 Dec. 7
No Lab
Last Day of Class
Paper 3 due
for all students		 Dec. 8
Dec. 9


Schedule of Experiments

Series Title Lab #
1 Worm Boot Camp; Autosomal and Sex-linked Traits 1-2
2 Classical (Forward) Genetics: Mutant Hunt, Linkage, Mapping, Complementation, DNA Sequence analysis 2-7
3 Reverse Genetics: Gene selection, Cloning & Making a feeding vector, RNAi 5-11

BISC219 F10 Weekly Lab Planner

Lab Date In-Lab Work Outside of Lab Work Assignment
1 Aug 30 to
Sept. 7 		LEARN WORM HUSBANDRY;
View worm videos;
Make your worm pick;
Examine worms: recognize different stages & sexes
Practice picking worms;
Start Series 1: Set up autosomal and X-linked crosses 		3 days after lab: pick (2) wild type worms from each cross to new plates (3 plates total);
Examine the phenotypes of the progeny – hermaphrodites and males - record all information in your notebook 		Homework: Complete Entry Survey; Familiarize yourself with the information in the BISC219/F11:Resources section;
Read all of the background information on C. elegans found in BISC219/F11:_Worm_Info and read the Lab 1 and Lab 2 information about our first Series 1: Autosomal vs. Sex-Linked Inheritance at BISC219/F11:_Gene_Linkage & BISC219/F11:_Lab_2; Complete Graded Assignment 1 explained at BISC219/F11: Assignment_1_Lab1
2 Sept. 8 to
Sept 14 		Complete Series 1: Count and examine phenotypes of autosomal vs. X-linked crosses;
Start Series 2: Classical (Forward) Genetics- Part 1- Examine mutant worms and compare to wild type:
Pick (3) putative Dpy mutants to separate plates 		3 days after lab:Examine mutants: check phenotype
if Dpy then cross L4 mutant hermaphrodites by L4 N2 males
(2 plates – duplicate) 		Homework: Read ALL of Series 2:background information BISC219/F11:_Gene_Mapping_Info and about all work to be performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our overall topic and experimental question(s) & goals. Outline the experimental process. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Assignment explained and rubric found at BISC219/F11: Assignment Series2_Outline_Summary

Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4.
Grading rubric & Assignment info at:
BISC219/F11: Assignment Help- Data Analysis 1

3 Sept. 15 to
Sept. 21 		Series 2- Part 2: Linkage Analysis: cross heterozygous males (+/d) from last cross to the (5) test strains (5 plates total);
 3 days after lab: Linkage: transfer (2) L4 hermaphrodites from each cross
to new plates (5 plates total) 		Homework: Draw crosses and diagram strategy for Series 2 Linkage Analysis due at the beginning of Lab 4. Information and grading rubric found at BISC219/F11: Assignment_Series2_Linkage Testing Crosses.

Con't Data Analysis (Results)of your autosomal vs. X-linked testing DUE at the beginning of LAB 4.
Grading rubric & Assignment info at:
BISC219/F11: Assignment Help- Data Analysis 1

4 Sept. 22 to
Sept. 28 		Complete Linkage Analysis: examine phenotypes and count to determine linkage;
Part 3: Start Mapping: pick (6) Uncs of the linked unc strain to separate plates (6 plates total);
Part 4: Start Complementation Analysis: Cross Dpy mutant worms to N2 males (2 plates total) 		3 days after lab: Mapping:
Pick (3) double mutants to separate plates (3 plates total);
Series2: Complementation: pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);
 Homework: Explain complementation analysis in general and specifically how it was used to id your dpy gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses using template provided. Due at the beginning of Lab 5. Assignment described atBISC219/F11: Assignment_Series2_Complementation
5 Sept. 29 to
Oct. 5 		Series2: Mapping:Self double mutants to keep viable true-breeding progeny;
Series2: Complementation: examine cross plates for Dpy males - WHY?; Series3: Reverse Genetics: Pick gene of interest; set up PCR reaction to clone the gene 		Series 3:Reverse Genetics: Examine the results of agarose gel electrophoresis Homework: Draw crosses and diagram strategy for Mapping the Location of your dpy mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 6. Assignment described at BISC219/F11: Assignment_Series2_Mapping Crosses
6 Oct. 12 to
Oct. 18 		Series 2: Mapping: cross N2 males (++/++) with L4 double mutant hermaphrodites (2 plates total);Self double mutants to new plate
Series2: Characterizing the dpy mutation Gene Sequence Analysis
Series3: Reverse Genetics:Restriction enzyme digest PCR product/clean-up/ligation into pPD129.36 vector/transformation into BL21 E.coli 		3 days after lab:Series2: Mapping: pick (4) male heterozygotes (++/d u) and Cross them with L4 hermaphrodite double mutants (d u/d u).
Series 3:Check control & transformation plates (save) –
notify instructor if no colonies 		Homework: DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at BISC219/F11: Assignment_Series2_DNA Sequencing
7 Oct. 19 to
Oct. 25 		Series2:Finish Mapping: SCORE! Calculate Recombination frequency;
Series3:Reverse Genetics Colony PCR to look for bacterial transformants 		Determine map distance between your mutant gene
and the known reference mutation
Day before lab: Series 3:grow overnight culture of positive colony 		Homework: Scientific research report on Series 2 Classical (Forward) Genetics Project due Fri. Nov. 4 for ALL students. 5%/day late penalty! See assignment directions at: BISC219/F11: Assignment_ Series2_Classical Genetics Paper
8 Nov. 2 to
Nov. 8 		Series3: Reverse Genetics: Plasmid isolation/quantification of DNA/ transformation of plasmid into E. coli strain HT115(DE3) Day before lab: grow overnight culture of single colony from transformation Homework: Write all Series 3 Protocols as Materials & Methods. Assignment described at BISC219/F11: Assignment_Series3_ Materials and Methods. Due at the beginning of Lab 9.
9 Nov. 9 to
Nov. 15 		Series3:RNAi : Induction of bacteria and seed plates for RNAi feeding;
Journal article discussion 		4 days later: Pick 2 L4 hermaphrodite worms of N2 and rrf-3 genotype to 2 RNAi plates for each genotype and make 1 control mock plate for each genotype (6 plates); Homework:Write a draft introduction section (including properly formatted Literature Cited page) of your next paper on our Reverse Genetics Project. Due at the beginning of Lab 10. Refer to BISC219/F11:Resources Guide to Scientific Writing.
10 Nov. 16 to
Nov. 22 		Series3:RNAi: SCORING (collection)of phenotype of fed worms compared to control N2 worms & worms with gene mutation -- Homework: Construct figures/tables with properly formatted legends to illustrate the main findings of the RNAi part of your Series3:Reverse Genetics project. These figures are to be used in a Science Writing workshop in Lab 11. Full paper on Series 3 Due last day of classes for all students. Read the original 1998 paper by Fire and Mello published in Nature 391: 806-811 (Feb.19 1998); Do your own library search to find and read a recent review article about RNAi in C. elegans. Also read the 2002 Simmer et al. paper in Current Biology 12:1317-1319 explaining the rrf-3 strain that we will use for our RNAi work. These articles provide background information on how the mechanism of RNAi was worked out experimentally and will be helpful to you when you write the introduction section of your Series3 paper. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A Caenorhabditis elegans Model PLoS ONE 4(8): e6860. This study, like yours, uses reverse genetics to investigate gene function.
NO LAB Nov. 23 to
Nov. 25 		Thanksgiving Break _
11 Nov. 28 to
Dec. 2 		Series 3:Reverse Genetics Science Writing Workshop & Effective Figure Design. Be sure to have read the original 1998 paper by Fire and Mello published in Nature 391: 806-811 (Feb.19 1998); Do your own library search to find and read a recent review article about RNAi in C. elegans. Also read the 2002 Simmer et al. paper in Current Biology 12:1317-1319 explaining the rrf-3 strain that we will use for our RNAi work. These articles provide background information on how the mechanism of RNAi was worked out experimentally and will be helpful to you when you write the introduction section of your Series3 paper. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A Caenorhabditis elegans Model PLoS ONE 4(8): e6860. This study, like yours, uses reverse genetics to investigate gene function. -- Homework: Full Scientific Research Report on Reverse Genetics Project – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References See BISC219/F11:Resources section Guide to Scientific Wriing and BISC219/F11: Assignment_Series3_Reverse Genetics Paper using RNAi.

DUE Dec. 7 by 5 pm for ALL students. Complete exit genetic assessment. See Sakai site for link and for how to obtain your incentive points.



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