BISC219/F11: Assignment Series2 Outline Summary

Assignment Series 2 Outline & Summary

Please construct a complete yet concise statement of the overall topic of investigation in Series 2 and the experimental question & goals of this classical (forward) genetics approach to understanding the structural and functional relationship between a gene and the product it encodes.

Construct and outline the entire experimental approach to achieving the our experimental goals in Series 2, including all of the following parts to our project: describing the mutant phenotype compared to wild type, linkage analysis, mapping the mutation's location to a chromosome and gene, complementation analysis and what it will tell you, defining the gene sequence change responsible for the gene product change that is associated with the functional defect in the mutant through DNA sequence analysis and comparison with wild type gene sequence, and bioinformatics using Wormbase or other BLAST bioinformatics to determine significance of the mutation in C. elegans or in other species through a search for homologues. This outline can be done as an outline or as a flow-diagram.

After you have outlined the experimental work in this project, please use that outline to construct a 1-2 page narrative summary in prose of how you will determine the exact location of a gene responsible for a particular mutant phenotype including finding the exact sequence change that is likely to be responsible and including the significance of the change in C. elegans and in other species. Note that this summary should NOT be a detailed list of exactly what you DO in lab in chronological order, but should, instead, be a brief summary of each part of the work that is included in our project. Imagine yourself trying to explain your project to someone who knows nothing about C. elegans genetics or about these mutant phenotypes.

Grading Rubric

Assignment Lab 2– 10 points

	At or Above Standard	Below Standard	Possible Points	Points Earned
Topic & Experimental Question	Constructed a clear, concise, and accurate description of the overall topic, the experimental question(s) and goals of the Series 2 investigation	Topic or experimental question(s)/goals missing, inaccurate, partially accurate; contains too much tangential information or is too simplified	2	__/2
Outline of Experimental Approach in Series 2: Classical or Forward Genetics	Constructed a complete, clear, concise, and accurate outline or flow diagram of ALL parts of Series2 including: mutant screen, linkage testing, mapping , complementation testing, determining if new mutation or previously characterized, DNA sequencing analysis, WormBase data base work	Outline/diagram is missing elements, is inaccurate or partially inaccurate; contains too much tangential information or is too simplified.	4	__/4
Narrative summary of project	Constructed a clear, concise, accurate, and logically developed description of the experimental approach to locating and characterizing the gene and gene mutation responsible for a phenotypic alteration from wild type	Experimental analysis missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, isn't logically developed	4	__/4
Total			10	__/10