BISC219/F12:Gene Mapping

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(New page: {{Template:BISC219/F12}} <div style="padding: 10px; width: 720px; border: 5px solid #7e354d;"> Background: Classical Forward Genetics and Gene Mapping...)
Current revision (09:39, 17 August 2012) (view source)
(Schedule of Experiments for Series 2)
 
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! 2
! 2
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|  
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| 9/11 - 9/17
| Picking mutant worms for genetic mapping
| Picking mutant worms for genetic mapping
| Confirm mutant phenotype and set up first cross
| Confirm mutant phenotype and set up first cross
|-
|-
! 3
! 3
-
|
+
| 9/18 - 9/24
| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites
| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites
| Pick hermaphrodites for selfing
| Pick hermaphrodites for selfing
|-
|-
! 4
! 4
-
|  
+
| 9/25 - 10/1
| Examine phenotypes to determine linkage<br>
| Examine phenotypes to determine linkage<br>
'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males
'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males
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! 5
! 5
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| 10/2 - 10/5 (10/15 Mon. Lab)
| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''
| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''
| Nothing! Enjoy Fall Break!
| Nothing! Enjoy Fall Break!
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! 6
! 6
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| 10/16 - 10/22
| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)  
| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)  
| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)  
| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)  
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! 7
! 7
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| 10/23 - 10/29
| ''' Complete Mapping'''<br>
| ''' Complete Mapping'''<br>
SCORE! Find recombinantion frequency
SCORE! Find recombinantion frequency
| Determine map distance between your Dpy and Unc marker<br>
| Determine map distance between your Dpy and Unc marker<br>
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|-

Current revision

Wellesley College BISC 219 Genetics

Home     People/Info     Lab Calendars/Weekly Planner     OWW Basics     Resources       Glossary    
Project 1:Sex-linked or Autosomal      Project 2:Classical (Forward) Genetics     Project 3: RNAi in Reverse Genetics
    Worm Info     Assignments       

Background: Classical Forward Genetics and Gene Mapping
Lab 2: Examining mutant worms for mapping
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2

Lab # Dates Activity Outside Activity
2 9/11 - 9/17 Picking mutant worms for genetic mapping Confirm mutant phenotype and set up first cross
3 9/18 - 9/24 Linkage crosses - heterozygous males with Unc hermaphrodites Pick hermaphrodites for selfing
4 9/25 - 10/1 Examine phenotypes to determine linkage

Mapping: Pick Unc non-Dpy to separate plates;
Complementation: cross unknown Dpy worms to wild type males

Mapping: Pick putative double mutants
Complementation: cross heterozygous males with known Dpy mutants
5 10/2 - 10/5 (10/15 Mon. Lab) Mapping: Maintain double mutants (d u/d u): pick a few to individual plates
Complementation: examine crosses for Dpy MALES
Nothing! Enjoy Fall Break!
6 10/16 - 10/22 DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;
Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species;
Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
7 10/23 - 10/29 Complete Mapping

SCORE! Find recombinantion frequency

Determine map distance between your Dpy and Unc marker