Melissa Beers: /* Schedule of Experiments for Series 2 */

2012-08-17T14:39:17Z

Schedule of Experiments for Series 2

←Older revision		Revision as of 14:39, 17 August 2012
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	\|-		\|-
	! 2		! 2
-	\|	+	\| 9/11 - 9/17
	\| Picking mutant worms for genetic mapping		\| Picking mutant worms for genetic mapping
	\| Confirm mutant phenotype and set up first cross		\| Confirm mutant phenotype and set up first cross
	\|-		\|-
	! 3		! 3
-	\|	+	\| 9/18 - 9/24
	\| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites		\| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites
	\| Pick hermaphrodites for selfing		\| Pick hermaphrodites for selfing
	\|-		\|-
	! 4		! 4
-	\|	+	\| 9/25 - 10/1
	\| Examine phenotypes to determine linkage<br>		\| Examine phenotypes to determine linkage<br>
	'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males		'''Mapping:''' Pick Unc non-Dpy to separate plates;<BR>'''Complementation:''' cross unknown Dpy worms to wild type males
Line 33:		Line 33:
	\|-		\|-
	! 5		! 5
-	\|	+	\| 10/2 - 10/5 (10/15 Mon. Lab)
	\| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''		\| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates<br>'''Complementation:''' examine crosses for Dpy '''MALES'''
	\| Nothing! Enjoy Fall Break!		\| Nothing! Enjoy Fall Break!
Line 39:		Line 39:
	\|-		\|-
	! 6		! 6
-	\|	+	\| 10/16 - 10/22
	\| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)		\| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;<br>'''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
	\| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)		\| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species;<br>'''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
	\|-		\|-
	! 7		! 7
-	\|	+	\| 10/23 - 10/29
	\| ''' Complete Mapping'''<br>		\| ''' Complete Mapping'''<br>
	SCORE! Find recombinantion frequency		SCORE! Find recombinantion frequency
	\| Determine map distance between your Dpy and Unc marker<br>		\| Determine map distance between your Dpy and Unc marker<br>
	\|-		\|-

Melissa Beers: New page: {{Template:BISC219/F12}}
Background: Classical Forward Genetics and Gene Mapping...

2011-12-13T00:52:37Z

New page: {{Template:BISC219/F12}} <div style="padding: 10px; width: 720px; border: 5px solid #7e354d;"> Background: Classical Forward Genetics and Gene Mapping...

New page

{{Template:BISC219/F12}}
<div style="padding: 10px; width: 720px; border: 5px solid #7e354d;">

[[BISC219/F12: Gene Mapping Info | Background: Classical Forward Genetics and Gene Mapping]] 
[[BISC219/F12: Lab 2 Mutant Hunt | Lab 2: Examining mutant worms for mapping]] 
[[BISC219/F12: Lab 3 | Lab 3: Linkage Test Part 1]] 
[[BISC219/F12: Lab 4 | Lab 4: Linkage Test Part 2, Mapping and Complementation]] 
[[BISC219/F12: Lab 5 | Lab 5: Mapping con't; complete complementation]] 
[[BISC219/F12: Lab 6 | Lab 6: DNA sequencing analysis; Mapping con't ]] 
[[BISC219/F12: Lab 7 | Lab 7: Complete Mapping: Score!]]

=='''Schedule of Experiments for Series 2'''==
{| border="1"
|+
! Lab # !! Dates !! Activity !! Outside Activity
|-
! 2
|
| Picking mutant worms for genetic mapping
| Confirm mutant phenotype and set up first cross
|-
! 3
|
| '''Linkage''' crosses - heterozygous males with Unc hermaphrodites
| Pick hermaphrodites for selfing
|-
! 4
|
| Examine phenotypes to determine linkage 
'''Mapping:''' Pick Unc non-Dpy to separate plates; '''Complementation:''' cross unknown Dpy worms to wild type males
| '''Mapping:''' Pick putative double mutants '''Complementation:''' cross heterozygous males with known Dpy mutants

|-
! 5
|
| '''Mapping:''' Maintain double mutants (d u/d u): pick a few to individual plates '''Complementation:''' examine crosses for Dpy '''MALES'''
| Nothing! Enjoy Fall Break!

|-
! 6
|
| ''' DNA Sequencing Analysis''':Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; '''Mapping''':Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
| '''Bioinformatics''': Use Wormbase and BLAST to learn more about the gene in other species; '''Mapping:'''Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
|-
! 7
|
| ''' Complete Mapping''' 
SCORE! Find recombinantion frequency
| Determine map distance between your Dpy and Unc marker 
|-

BISC219/F12:Gene Mapping - Revision history

Melissa Beers: /* Schedule of Experiments for Series 2 */

Melissa Beers: New page: {{Template:BISC219/F12}} Background: Classical Forward Genetics and Gene Mapping...

Melissa Beers: New page: {{Template:BISC219/F12}}
Background: Classical Forward Genetics and Gene Mapping...