BISC219/F12:Gene Mapping
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Background: Classical Forward Genetics and Gene Mapping
Lab 2: Examining mutant worms for mapping
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!
Schedule of Experiments for Series 2
| Lab # | Dates | Activity | Outside Activity |
|---|---|---|---|
| 2 | Picking mutant worms for genetic mapping | Confirm mutant phenotype and set up first cross | |
| 3 | Linkage crosses - heterozygous males with Unc hermaphrodites | Pick hermaphrodites for selfing | |
| 4 | Examine phenotypes to determine linkage Mapping: Pick Unc non-Dpy to separate plates; |
Mapping: Pick putative double mutants Complementation: cross heterozygous males with known Dpy mutants | |
| 5 | Mapping: Maintain double mutants (d u/d u): pick a few to individual plates Complementation: examine crosses for Dpy MALES |
Nothing! Enjoy Fall Break! | |
| 6 | DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du) |
Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species; Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u) | |
| 7 | Complete Mapping SCORE! Find recombinantion frequency |
Determine map distance between your Dpy and Unc marker |
