BISC219/F12:RNA interference: Difference between revisions
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[[BISC219/F12: RNAi General Information| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br> | [[BISC219/F12: RNAi General Information| Background Information on Project 3: Investigating Gene Regulation Using RNAi]] <br> | ||
[[BISC219/F12: Media Recipes | Media Recipes]]<br> | [[BISC219/F12: Media Recipes | Media Recipes]]<br> | ||
[[BISC219/F12: RNAi Lab 7 | Lab 7: ]]<br> | [[BISC219/F12: RNAi Lab 7 | Lab 7: Identifying a bacterial colony containing our plasmid of interest ]]<br> | ||
[[BISC219/F12: RNAi Lab 8 | Lab 8: ]]<br> | [[BISC219/F12: RNAi Lab 8 | Lab 8: Creating the feeding strain of bacteria for RNAi]]<br> | ||
[[BISC219/F12: RNAi Lab 9 | Lab 9: ]]<br> | [[BISC219/F12: RNAi Lab 9 | Lab 9: Induction of feeding strain to produce dsRNA and feeding worms]]<br> | ||
[[BISC219/F12: RNAi Lab 10 | Lab 10: ]]<br> | [[BISC219/F12: RNAi Lab 10 | Lab 10: Phenotypic analysis of treated vs untreated worms]]<br> | ||
[[BISC219/F12: RNAi Lab 11 | Lab 11: ]]<br> | [[BISC219/F12: RNAi Lab 11 | Lab 11: Writing Workshop]]<br> | ||
[[BISC219/F12: RNAi Lab 12 | Lab 12: ]]<br> | [[BISC219/F12: RNAi Lab 12 | Lab 12: Writing Conferences]]<br> | ||
<br> | <br> | ||
Revision as of 18:40, 21 August 2012
These labs were modified from the work of Janet Andersen, Alexander Krichevsky, Joerg R. Leheste, and Daniel J. Moloney at the Dept. of Biochemistry and Cellular BIology at Stony Brook University with help from the Silencing Genomes project of the Dolan DNA Learning Center in Cold Spring Harbour, New York.
Background Information on Project 3: Investigating Gene Regulation Using RNAi
Media Recipes
Lab 7: Identifying a bacterial colony containing our plasmid of interest
Lab 8: Creating the feeding strain of bacteria for RNAi
Lab 9: Induction of feeding strain to produce dsRNA and feeding worms
Lab 10: Phenotypic analysis of treated vs untreated worms
Lab 11: Writing Workshop
Lab 12: Writing Conferences
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 7 | 10/23 - 10/29 | Picking a colony of bacteria containing the RNAi plasmid PCR to confirm presence of gene of interest |
The night before next lab: set up an overnight culture of your colony of interest |
| 8 | 10/30 - 11/5 | Miniprep of plasmid and transformation into feeding bacteria strain | The day after lab: check your transformed bacteria for colony growth The night before next lab: set up an overnight culture of your colony of interest |
| 9 | 11/7 - 11/13 | Induction of dsRNA production, seeding of RNAi plates and addition of worms | |
| 10 | 11/14 - 11/20 | Chemotaxis Assay | |
| 11 | 11/26 - 11/30 | Writing Workshop | |
| 12 | 12/3 - 12/7 | Writing Conferences |
