BISC220/S10: Lab Calendars/Weekly Planner: Difference between revisions
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| | | Enzyme Structure/Function | ||
| 1-5 | | 1-5 | ||
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!2 | !2 | ||
| | | Yeast Genetics and the Secretory Pathway | ||
| | | 6-8 | ||
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!3 | !3 | ||
| | | Cell Culture and the Cytoskeleton | ||
| | | 9-12 | ||
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Revision as of 08:30, 5 January 2010
BISC220 S10 Lab Calendar
Monday | Tuesday | Wednesday | Thursday | Friday | |
---|---|---|---|---|---|
Jan. 25 |
Jan. 26 Lab 1 Enzymes 1 |
Jan. 27 Lab 1 Enzymes 1 |
Jan. 28 Lab 1 Enzymes 1 |
Jan. 29 |
|
Feb. 1 |
Feb. 2 Lab 2 Enzymes 2 |
Feb. 3 Lab 2 Enzymes 2 |
Feb. 4 Lab 2 Enzymes 2 |
Feb. 5 |
|
Feb. 8 |
Feb. 9 Lab 3 Enzymes 3 |
Feb. 10 Lab 3 Enzymes 3 |
Feb. 11 Lab 3 Enzymes 3 |
Feb. 12 |
|
Feb. 15 President's Day |
Feb. 16 Monday Schedule NO LAB |
Feb. 17 NO LAB |
Feb. 18 NO LAB |
Feb. 19 |
|
Feb. 22 |
Feb. 23 Lab 4 Enzymes 4 |
Feb. 24 Lab 4 Enzymes 4 |
Feb. 25 Lab 4 Enzymes 4 |
Feb. 26 |
|
Mar. 1 |
Mar. 2 Lab 5 Science Writing |
Mar. 3 Lab 5 Science Writing |
Mar. 4 Lab 5 Science Writing |
Mar. 5 |
|
Mar. 8 |
Mar. 9 Lab 6 Yeast 1 Paper 1 due |
Mar. 10 Lab 6 Yeast 1 Paper 1 due |
Mar. 11 Lab 6 Yeast 1 Paper 1 due |
Mar. 12 |
|
Mar. 15 |
Mar. 16 Lab 7 Yeast 2 |
Mar. 17 Lab 7 Yeast 2 |
Mar. 18 Lab 7 Yeast 2 |
Mar. 19 |
|
Mar. 22 Spring Break |
Mar. 23 Spring Break |
Mar. 24 Spring Break |
Mar. 25 Spring Break |
Mar. 26 Spring Break |
|
Mar. 29 |
Mar. 30 Lab 8 Yeast 3 |
Mar. 31 Lab 8 Yeast 3 |
April 1 Lab 8 Yeast 3 |
April 2 |
|
April 5 |
April 6 Lab 9 Cell Culture |
April 7 Lab 9 Cell Culture |
April 8 Lab 9 Cell Culture |
April 9 |
|
April 12 |
April 13 Lab 10 Cytoskeleton 1 Paper 2 due |
April 14 Lab 10 Cytoskeleton 1 Paper 2 due |
April 15 Lab 10 Cytoskeleton 1 Paper 2 due |
April 16 |
|
April 19 Patriot's Day |
April 20 Lab 11 Cytoskeleton 2 |
April 21 Lab 11 Cytoskeleton 2 |
April 22 Monday Schedule |
April 23 |
|
April 26 |
April 27 Lab 12 Presentations |
April 28 Rhulman NO LAB |
April 29 Lab 12 Presentations |
April 30 |
|
May 3 |
May 4 NO LAB |
May 5 Lab 12 Presentations |
May 6 Last day of classes Paper 3 due |
Schedule of Experiments
Series | Title | Lab # |
---|---|---|
1 | Enzyme Structure/Function | 1-5 |
2 | Yeast Genetics and the Secretory Pathway | 6-8 |
3 | Cell Culture and the Cytoskeleton | 9-12 |
Graded Assignments
Lab Assigned | Lab Due | Assignment description |
Point Value (Total 200) |
---|---|---|---|
Lab 2 | Lab 3 | Autosomal vs. X-linked analysis | 15 |
Lab 5 | Friday, October 23rd by 5 pm | Gene Mapping Paper | 40 |
Lab 5 | Wednesday, October 14th by 5 pm Monday Lab ONLY: Monday October 19th by 5 pm |
Materials and Methods Write-up: Two M&M sections for your paper |
15 |
Lab 8 | Lab 9 | Sequence Analysis write up | 10 |
Lab 8 | Tuesday, November 17th by 5 pm | RNAi paper - Full paper | 45 |
Lab 9 | Wednesday, December 2nd by 5 pm | Revision of Paper #2 | 20 |
Lab 10 | Friday, December 11th by 5 pm | Transgenic Plant Paper - Science Style | 50 |
Other | Discretionary Points: Preparation & Participation |
5 |
BISC219 F09 Weekly Lab Planner
Lab | Date | In-Lab Work | Outside of Lab Work | Assignment |
---|---|---|---|---|
1 | Tues. 9/9 to Mon. 9/14 |
Start tobacco transformation Examine C. elegans and learn to identify the stages and sexes Practice picking worms Set up your first crosses to examine autosomal vs. X-linkage (3 plates total) Perform a mutant hunt: Pick (3) Dumpy mutants to separate plates (3 plates total) |
3-5 days after Lab 1 transfer tobacco leaf tissue to new media with antibiotics 3-4 days after setting up your crosses pick 2 wild type worms from each autosomal vs. X-linked cross to new plates (3 plates total) be sure to examine the F1 progeny taking note of male and hermaphrodite phenotypes Examine mutants from hunt - check phenotype. If Dumpy then cross L4 mutant hermaphrodites by L4 wild type (N2) males (2 plates - duplicates) |
Familiarize yourself with the information in the BISC_219/2009:Resources section. Read the Introduction, Tools and Techniques, and first two labs in the Gene Mapping series: BISC 219/2009:Gene Mapping Read the Background and Creating the Transgenic Plants in the Creating a Transgenic Organism series: BISC 219/2009:Creating a Transgenic Organism |
2 | Tue. Sept. 15 to Mon. Sept 21 |
Calibrate micropipettes Count and examine phenotypes of autosomal vs. X-linked crosses Linkage: cross males from last cross to the (4) test strains (4 plates total) |
3 days after lab: Linkage: transfer (2) L4 hermaphrodites from each cross to 2 new plates (8 plates total) |
Homework:Data Analysis (Results)of your autosomal vs. X-linked testing (15pt) due next lab. Grading rubric & Assignment info at: BISC_219: Assignment Help- Data Analysis 1 |
3 | Tues. Sept. 22 to Mon. Sept. 28 |
Linkage: examine phenotypes and count to determine linkage Mapping: pick (5) Dpy worms from linkage plate to separate plates (5 plates total) |
3 days after lab: Mapping: Pick (3) double mutants to separate plates (3 plates total) Complementation: Cross Dpy mutant worms to N2 males (2 plates total) |
Homework: Read journal article #1 for discussion in Lab 4: Bruinsma et al., Identification of Mutations in Caenorhabditis elegans That Cause Resistance to High Levels of Dietary Zinc and Analysis Using a Genomewide Map of Single Nucleotide Polymorphisms Scored by Pyrosequencing. Genetics. 2008 June; 179(2): 811–828.
doi: 10.1534/genetics.107.084384. Full text available from PubMed or from Wellesley College Library at [1] |
4 | Tues. Sept. 29 to Mon. Oct. 5 |
Plant: Transfer tobacco shoots to root inducing medium Mapping: cross N2 males with double mutants (2 plates total) Complementation: pick males from complementation cross #1 and mate with known Dpy strains (4 plates total) RNAi: pick a single colony – circle and save for next week Discuss Paper #1 |
3 days after lab: Mapping: pick 3-4 males from previous cross to 2 plates and cross with d u/d u hermaphrodites for a test cross (2 plates total) Complementation: examine cross plates for Dpy males Set up an overnight culture of your colony on the day before next lab |
Homework: Start working on Mapping Partial Paper (Introduction section). See BISC_219/2009:Resources and BISC_219: Assignment Help- Partial Paper Mapping for help. |
5 | Tues. Oct. 6 to Wed. Oct. 14 |
Mapping: SCORE RNAi: Plasmid DNA isolation (mini-prep) Transform the miniprep into the feeding bacterial strain |
Determine map distance between your mutant gene and the known reference mutation. Make sure all transformations are successful. If not, contact instructor to problem solve The day before lab: Set up an overnight culture of your transformation |
Homework: Work on Gene Mapping Partial paper – Title, Abstract, Intro, Results, References – due 10/23. See BISC_219/2009:Resources and BISC_219: Assignment Help- Partial Paper Mapping for help. Write up practice M &M sections (15 points) on plasmid isolation and transformation BISC_219: Assignment Help- Materials and Methods – due Wednesday October 14th by 5 pm Read journal articles: |
6 | Mon. Oct. 19 to Fri. Oct. 23 |
RNAi: induction of bacteria for RNAi feeding Seed plates with induced bacteria and allow to dry Paper discussion |
4 days after lab: Add 2 L4 worms to your plates – N2, rrf-3 |
Homework: Gene Mapping partial paper due Friday October 23rd by 5 pm for all students |
7 | Mon. Oct. 26 to Fri. Oct. 30 |
Plant:Transfer plantlets to soil and remove them from their sterile world RNAi: examine the three sets of worms and determine their phenotypes Set up single worm PCR RNAi treated and mutant worms |
Complete Practice sequencing exercise | Homework: Work on M&M revisions for RNAi paper and additional M&M protocols |
8 | Wed. Nov. 4 to Tues. Nov. 10 |
RNAi: Clean up PCR reactions Run sequencing reaction and cleanup (columns) Load samples into Sequencer |
Instructors will post your sequencing data to the conference – analyze your results Examine and score histochemial data according to instructors directions |
Homework: Write up practice sequencing data analysis (10 points). BISC_219: Assignment Help- Sequence Analysis Due in lab next week. Read for next lab: Apse, G.P., Aharon, G.S., Snedden, W.A., Blumwald, E. "Salt Tolerance Conferred by Overexpression of a Vacuolar Na+/H+ Antiport in Arabidopsis" Science (1999) 285: 1256-1258. Available at: http://www.sciencemag.org/cgi/content/full/285/5431/1256 or in pdf form at http://0-www.sciencemag.org.luna.wellesley.edu:80/cgi/reprint/285/5431/1256.pdf and the Science perspectives article by Frommer Taking Transgenic Plants with a Pinch of Salt, Science 20 August 1999: Vol. 285. no. 5431, pp. 1222 - 1223. DOI: 10.1126/science.285.5431.1222 at [5]
|
9 | Wed. Nov. 11 to Tues. Nov. 17 |
Plants: Restriction enzyme digest of PCR reactions Agarose gel electrophoresis of cut PCR product Paper Discussion #3 |
_ | Work on Paper #2 – Full Paper – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References – BISC_219: Assignment Help- RNAi full paper- DUE Nov. 17th at 5 pm |
10 | Wed. Nov. 18 to Tues. Nov. 24 |
Plants:Spectrophotometric GUS enzyme activity assay Phenotypic analysis of plants Paper #2 review returned in lecture 11/24 for revision Discuss The Science of Science Writing by Gopen and Swan from the American Scientist (Nov.-Dec. 1990, Vol78, 550-558 (Available in the E-Reserves folder on the BISC219 course First Class) conference |
_ | Homework: Analyze all data & create figures/tables from course gusA and GUS data for the Workshop. Assignment help at BISC 219/2009: Mod 3 Analyzing the data on the putative transgenic and control plants
|
11 | Mon. Nov. 30 to Fri. Dec 4 |
Data Analysis:Effective Figure Design Workshop | _ | Homework: Write final paper on Genetic Engineering in Science style. Due: December 11, 2009 by 5 pm. Instructions at BISC 219: Assignment Help- Transgenic plants research report |
12 | Mon. Dec.7 to Fri. Dec. 11 |
TBA | TBA | TBA |