BISC220/S10: Lab Calendars/Weekly Planner: Difference between revisions

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==BISC220 S10 Lab Calendar==
==BISC220 S10 Lab Calendar==
Line 10: Line 10:
|-
|-
|  Jan. 25 <br><br>
|  Jan. 25 <br><br>
| Jan. 26 <br>Lab 1<br> Enzymes 1 ||Jan. 27  <br> Lab 1<br> Enzymes 1 || Jan. 28  <br> Lab 1<br> Enzymes 1 || Jan. 29 <br><br> ||
| Jan. 26 <br>Lab 1<br> Enzymes 1  
| Jan. 27  <br> Lab 1<br> Enzymes 1
| Jan. 28  <br> Lab 1<br> Enzymes 1  
| Jan. 29 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>  
|-
|-
| Feb. 1<br><br>  
| Feb. 1<br><br>  
| Feb. 2 <br>Lab 2 <br> Enzymes 2|| Feb. 3 <br>Lab 2<br>Enzymes 2 || Feb. 4 <br>Lab 2<br>Enzymes 2 || Feb. 5 <br><br> || 
| Feb. 2 <br>Lab 2 <br> Enzymes 2
| Feb. 3 <br>Lab 2<br>Enzymes 2  
| Feb. 4 <br>Lab 2<br>Enzymes 2  
| Feb. 5 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>  
|-
|-
| Feb. 8 <br><br>
| Feb. 8 <br><br>
| Feb. 9 <br>Lab 3<br> Enzymes 3 || Feb. 10 <br>Lab 3<br> Enzymes 3 ||  Feb. 11 <br>Lab 3<br> Enzymes 3 ||Feb. 12 <br><br> || 
| Feb. 9 <br>Lab 3<br> Enzymes 3  
| Feb. 10 <br>Lab 3<br> Enzymes 3  
|  Feb. 11 <br>Lab 3<br> Enzymes 3  
| Feb. 12 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>  
|-
|-
| Feb. 15 <br>'''President's Day'''<br>
| Feb. 15 <br>'''President's Day'''<br>
| Feb. 16 <br>'''Monday Schedule'''<br> '''NO LAB''' || Feb. 17 <br> '''NO LAB''' <br> ||  Feb. 18 <br> '''NO LAB''' <br>|| Feb. 19 <br><br>|| 
| Feb. 16 <br>'''Monday Schedule'''<br> '''NO LAB'''  
| Feb. 17 <br> '''NO LAB''' <br>  
|  Feb. 18 <br> '''NO LAB''' <br>
| Feb. 19 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>
|-
|-
| Feb. 22 <br><br>
| Feb. 22 <br><br>
| Feb. 23 <br>Lab 4 <br> Enzymes 4 || Feb. 24 <br>Lab 4 <br> Enzymes 4 ||  Feb. 25 <br>Lab 4 <br> Enzymes 4 || Feb. 26 <br><br> || 
| Feb. 23 <br>Lab 4 <br> Enzymes 4  
| Feb. 24 <br>Lab 4 <br> Enzymes 4  
| Feb. 25 <br>Lab 4 <br> Enzymes 4  
| Feb. 26 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>  
|-
|-
| Mar. 1 <br> <br>  
| Mar. 1 <br> <br>  
| Mar. 2 <br>Lab 5 <br> '''Science Writing'''  || Mar. 3 <br> Lab 5 <br> '''Science Writing''' ||  Mar. 4 <br>Lab 5 <br> '''Science Writing''' || Mar. 5 <br><br>|| 
| Mar. 2 <br>Lab 5 <br> '''Science Writing'''   
| Mar. 3 <br> Lab 5 <br> '''Science Writing'''  
| Mar. 4 <br>Lab 5 <br> '''Science Writing'''  
| Mar. 5 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>
|-
|-
| Mar. 8 <br><br>
| Mar. 8 <br><br>
| Mar. 9 <br>Lab 6<br> Yeast 1<br> '''''Paper 1 due''''' || Mar. 10  <br>Lab 6<br> Yeast 1<br> '''''Paper 1 due'''''  ||  Mar. 11 <br>Lab 6<br> Yeast 1<br> '''''Paper 1 due''''' || Mar. 12 <br><br>|| 
| Mar. 9 <br>Lab 6<br> Yeast 1<br> '''''Paper 1 due'''''  
| Mar. 10  <br>Lab 6<br> Yeast 1<br> '''''Paper 1 due'''''   
| Mar. 11 <br>Lab 6<br> Yeast 1<br> '''''Paper 1 due'''''  
| Mar. 12 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>  
|-
|-
| Mar. 15 <br><br>
| Mar. 15 <br><br>
| Mar. 16 <br>Lab 7<br> Yeast 2 || Mar. 17<br>Lab 7<br>Yeast 2  ||  Mar. 18 <br>Lab 7<br> Yeast 2 || Mar. 19 <br><br>|| 
| Mar. 16 <br>Lab 7<br> Yeast 2  
| Mar. 17<br>Lab 7<br>Yeast 2   
| Mar. 18 <br>Lab 7<br> Yeast 2  
| Mar. 19 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>
|-
|-
| Mar. 22 <br>'''Spring Break'''
| Mar. 22 <br>'''Spring Break'''
| Mar. 23 <br> '''Spring Break'''|| Mar. 24 <br>'''Spring Break''' ||  Mar. 25 <br>'''Spring Break''' || Mar. 26<br>'''Spring Break'''|| 
| Mar. 23 <br> '''Spring Break'''
| Mar. 24 <br>'''Spring Break'''  
| Mar. 25 <br>'''Spring Break'''  
| Mar. 26 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br>'''Spring Break'''  
|-
|-
| Mar. 29 <br> <br>
| Mar. 29 <br> <br>
| Mar. 30 <br>Lab 8 <br> Yeast 3|| Mar. 31  <br>Lab 8 <br> Yeast 3 || April 1 <br>Lab 8  <br> Yeast 3 || April 2 <br><br>|| 
| Mar. 30 <br>Lab 8 <br> Yeast 3
| Mar. 31  <br>Lab 8 <br> Yeast 3  
| April 1 <br>Lab 8  <br> Yeast 3  
| April 2 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>
|-
|-
| April 5 <br> <br>
| April 5 <br> <br>
| April 6 <br>Lab 9 <br> Cell Culture || April 7  <br>Lab 9 <br> Cell Culture  ||  April 8 <br>Lab 9 <br> Cell Culture  || April 9 <br><br>|| 
| April 6 <br>Lab 9 <br> Cell Culture  
| April 7  <br>Lab 9 <br> Cell Culture   
| April 8 <br>Lab 9 <br> Cell Culture   
| April 9 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>  
|-
|-
| April 12 <br>
| April 12 <br>
| April 13<br>Lab 10 <br> Cytoskeleton 1 <br> '''''Paper 2 due''''' || April 14 <br>Lab 10 <br> Cytoskeleton 1 <br> '''''Paper 2 due''''' ||  April 15 <br>Lab 10 <br> Cytoskeleton 1 <br> '''''Paper 2 due''''' || April 16 <br><br>||  
| April 13<br>Lab 10 <br> Cytoskeleton 1 <br> '''''Paper 2 due'''''  
| April 14 <br>Lab 10 <br> Cytoskeleton 1 <br> '''''Paper 2 due'''''  
| April 15 <br>Lab 10 <br> Cytoskeleton 1 <br> '''''Paper 2 due'''''  
| April 16 <br><br>   
|-
|-
| April 19 <br> '''Patriot's Day'''
| April 19 <br> '''Patriot's Day'''
| April 20 <br>Lab 11<br> Cytoskeleton 2 || April 21 <br>Lab 11 <br> Cytoskeleton 2 ||  April 22 <br>'''Monday Schedule''' || April 23 <br><br>|| 
| April 20 <br>Lab 11<br> Cytoskeleton 2  
| April 21 <br>Lab 11 <br> Cytoskeleton 2  
| April 22 <br>'''Monday Schedule'''  
| April 23 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>
|-
|-
| April 26 <br> <br>
| April 26 <br> <br>
| April 27 <br>Lab 12<br> Presentations || April 28 <br> '''Rhulman'''<br> '''NO LAB'''||  April 29 <br>Lab 12 <br> Presentations || April 30 <br><br>||  
| April 27 <br>Lab 12<br> Presentations  
| April 28 <br> '''Rhulman'''<br> '''NO LAB'''
| April 29 <br>Lab 12 <br> Presentations  
| April 30 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>   
|-
|-
| May 3 <br><br>
| May 3 <br><br>
| May 4 <br> '''NO LAB''' || May 5 <br> Lab 12 <br> Presentations || May 6 <br> '''Last day of classes''' <br> '''''Paper 3 due'''''
| May 4 <br> '''NO LAB'''  
| May 5 <br> Lab 12 <br> Presentations  
| May 6 <br> '''Last day of classes''' <br> '''''Paper 3 due'''''  
| May 7 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br><br>
|-
|-
|}
|}
Line 63: Line 108:
|-
|-
! 1  
! 1  
| Gene Mapping
| Enzyme Structure/Function
| 1-5   
| 1-5   
|-
|-
!2  
!2  
| RNA interference
| Yeast Genetics and the Secretory Pathway
| 5-8
| 6-8
|-
|-
!3  
!3  
| Plant Genetic Engineering
| Cell Culture and the Cytoskeleton
| 1,4,7-10
| 9-12
|-
|-
|}
|}
<br>
<br>


==Graded Assignments==
==Graded Assignments==
Line 80: Line 127:
|+  
|+  
! Lab Assigned !! Lab Due !! Assignment<br> description !! Point Value <br>(Total 200)
! Lab Assigned !! Lab Due !! Assignment<br> description !! Point Value <br>(Total 200)
|-
! Lab 1
| Lab 2
| RasMol/ClustalW
| 10
|-
|-
! Lab 2
! Lab 2
| Lab 3  
| Lab 3
| Autosomal vs. X-linked analysis
| Calculations from Lab 2
| 10
|-
! Lab 3
| Lab 4
| Calculations from Lab 3
| 15
| 15
|-
|-
! Lab 5
! Lab 4
| Friday, October 23rd by 5 pm
| Lab 6 <br> Week of March 8th
| Gene Mapping Paper
| β-galactosidase paper
| 40
| 40
|-
|-
! Lab 5
! Lab 6
| Wednesday, October 14th by 5 pm <br>
| Lab 7
'''Monday Lab ONLY:'''  Monday October 19th by 5 pm<br>
| Yeast growth predictions/BLAST
|Materials and Methods Write-up: <br> Two M&M sections for your paper
| 15
| 15
|-
|-
! Lab 8
! Lab 8
| Lab 9
| Lab 10 <br> Week of April 12
| Sequence Analysis write up
| Yeast Secretory Pathway Paper
| 10
|-
! Lab 8
| Tuesday, November 17th by 5 pm
| RNAi paper - Full paper
| 45
| 45
|-
|-
! Lab 9
! Lab 10
| Wednesday, December 2nd by 5 pm
| Lab 12
| Revision of Paper #2
| Cytoskeleton Imaging Oral Presentations
| 20
| 25
|-
|-
! Lab 10
! Lab 11
| Friday, December 11th by 5 pm
| May 6 - Last Day of Classes
| Transgenic Plant Paper - Science Style
| Cytoskeleton Partial Paper
| 50
| 35
|-
|-
! Other
! Other
|  
|
| Discretionary Points: <br> Preparation & Participation
| "P" Points - Participation and Preparation
| 5
| 5
|-
|}
 
<br>
 
 
==BISC219 F09 Weekly Lab Planner==
{| border="1"
|+
! '''Lab''' !! '''Date''' !! '''In-Lab Work''' !! '''Outside of Lab Work''' !! '''Assignment'''
|-
! 1
| Tues. 9/9 to <br>Mon. 9/14
| Start tobacco transformation <br><br>Examine ''C. elegans'' and learn to identify the stages and sexes <br><br> Practice picking worms<br><br>Set up your first crosses to examine autosomal vs. X-linkage ('''3 plates total)''' <br><br> Perform a mutant hunt: Pick (3) Dumpy mutants to separate plates ('''3 plates total''')
|3-5 days after Lab 1 transfer tobacco leaf tissue to new media '''''with''''' antibiotics<br><br> 3-4 days after setting up your crosses pick 2 wild type worms from each autosomal vs. X-linked cross to new plates ('''3 plates total''') be sure to examine the F1 progeny taking note of male and hermaphrodite phenotypes <br><br> Examine mutants from hunt - check phenotype.  If '''Dumpy''' then cross L4 mutant hermaphrodites by L4 wild type (N2) males ('''2 plates - duplicates''')
| Familiarize yourself with the information in the [[BISC_219/2009:Resources]] section. <br><br> Read the Introduction, Tools and Techniques, and first two labs in the '''Gene Mapping''' series: [[BISC 219/2009:Gene Mapping]]<br><br>Read the Background and Creating the Transgenic Plants in the '''Creating a Transgenic Organism''' series: [[BISC 219/2009:Creating a Transgenic Organism]]
|-
! 2
| Tue. Sept. 15 to <br> Mon. Sept 21
| Calibrate micropipettes <br><br>Count and examine phenotypes of autosomal vs. X-linked crosses <br><br> '''Linkage''': cross males from last cross to the (4) test strains ('''4 plates total''')
|'''3 days after lab:'''<br> '''Linkage''': transfer (2) L4 hermaphrodites from each cross to 2 new plates ('''8 plates total''')
| '''Homework:'''Data Analysis (Results)of your autosomal vs. X-linked testing (15pt) due next lab. Grading rubric & Assignment info at: [[BISC_219: Assignment Help- Data Analysis 1]]
|-
! 3
| Tues. Sept. 22 to <br> Mon. Sept. 28
| '''Linkage''': examine phenotypes and count to determine linkage <br><br> '''Mapping''': pick (5) Dpy worms from linkage plate to separate plates ('''5 plates total''')
| '''3 days after lab:''' <br> '''Mapping''': Pick (3) double mutants to separate plates ('''3 plates total''')<br><br> '''Complementation''': Cross Dpy mutant worms to N2 males ('''2 plates total''')
| '''Homework:''' Read journal article #1 for discussion in Lab 4: Bruinsma et al., Identification of Mutations in Caenorhabditis elegans That Cause Resistance to High Levels of Dietary Zinc and Analysis Using a Genomewide Map of Single Nucleotide Polymorphisms Scored by Pyrosequencing. ''Genetics.'' 2008 June; 179(2): 811–828.
doi: 10.1534/genetics.107.084384. Full text available from PubMed or from Wellesley College Library at [http://luna.wellesley.edu/search~S3?/tGenetics/tgenetics/1,42,45,B/l856~b1919161&FF=tgenetics&2,,4,2,0]
|-
! 4
| Tues. Sept. 29 to <br> Mon. Oct. 5
| '''Plant''': Transfer tobacco shoots to root inducing medium <br><br> '''Mapping''': cross N2 males with double mutants ('''2 plates total''')<br><br>'''Complementation''':  pick males from complementation cross #1 and mate with known Dpy strains ('''4 plates total''') <br><br> '''RNAi''':  pick a single colony – circle and save for next week <br><br> Discuss Paper #1
| '''3 days after lab:'''<br> '''Mapping''': pick 3-4 males from previous cross to 2 plates and cross with d u/d u hermaphrodites for a test cross ('''2 plates total''') <br><br> '''Complementation''': examine cross plates for Dpy males <br><br> Set up an overnight culture of your colony on the day before next lab
| '''Homework:''' Start working on Mapping Partial Paper (Introduction section). See [[BISC_219/2009:Resources]] and [[BISC_219: Assignment Help- Partial Paper Mapping]] for help.
|-
! 5
| Tues. Oct. 6 to<br> Wed. Oct. 14
| '''Mapping''':  SCORE <br><br> '''RNAi''': Plasmid DNA isolation (mini-prep) <br><br> Transform the miniprep into the feeding bacterial strain
| Determine map distance between your mutant gene and the known reference mutation. <br><br> Make sure all transformations are successful.  If not, contact instructor to problem solve <br><br> '''The day before lab''':  Set up an overnight culture of your transformation
| '''Homework:''' Work on Gene Mapping Partial paper – Title, Abstract, Intro, Results, References – '''due 10/23'''.  See [[BISC_219/2009:Resources]] and [[BISC_219: Assignment Help- Partial Paper Mapping]] for help. <br><br> Write up practice M &M sections (15 points) on plasmid isolation and transformation [[BISC_219: Assignment Help- Materials and Methods]] – '''due Wednesday October 14th by 5 pm'''<br><br>
Read journal articles:<br>
1) Fire et al, ''Potent and specific genetic interference by ds RNA in C. elegans'' in ''Nature'' Feb.19,1998 Vol.391 pages 806-811 (doi:10.1038/nature07759. Available at: [http://www.nature.com/nature/journal/v391/n6669/index.html]<br>
2)Steiner and Plasterk's preview of the Yigid article, ''Knocking out the Argunautes'', found in ''Cell'' 127, Nov. 17, 2006, DOI: doi:10.1016/j.cell.2006.11.004  available at: [http://www.cell.com/archive?year=2006]<br>
3) Yigid et al, ''Analysis of the C. elegans Argonaute Family Reveals that Distinct Argonautes Act Sequentially during RNAi'' in ''Cell'' Volume 127, Issue 4, 17 November 2006, Pages 747-757, (doi:10.1016/j.cell.2006.09.033), available at [http://www.cell.com/archive?year=2006] for discussion in Lab 6.
|-
! 6
| Mon. Oct. 19 to <br> Fri. Oct. 23
| '''RNAi''': induction of bacteria for RNAi feeding <br><br> Seed plates with induced bacteria and allow to dry <br><br> Paper discussion
| '''4 days after lab:'''<br> Add 2 L4 worms to your plates – N2, ''rrf-3''
| '''Homework''': Gene Mapping partial paper due Friday October 23rd by 5 pm for all students
|-
! 7
| Mon. Oct. 26 to <br> Fri. Oct. 30
| '''Plant''':Transfer plantlets to soil and remove them from their sterile world <br><br> '''RNAi''': examine the three sets of worms and determine their phenotypes <br>
Set up single worm PCR RNAi treated and mutant worms <br><br> Run agarose gel of PCR results <br><br> Practice sequencing data analysis with ''Sequencher'' software
| Complete Practice sequencing exercise
| '''Homework''': Work on M&M revisions for RNAi paper and additional M&M protocols
|-
! 8
| Wed. Nov. 4 to <br> Tues. Nov. 10
| '''RNAi''': Clean up PCR reactions <br><br> Run sequencing reaction and cleanup (columns)<br><br> Load samples into
Sequencer <br><br>  '''Plants''': Plant chromosomal DNA extraction from leaf tissue <br><br> PCR amplification of transgene <br><br> Histochemical GUS enzyme activity assay
| Instructors will post your sequencing data to the conference – analyze your results<br><br> Examine and score histochemial data according to instructors directions
| '''Homework''': Write up practice sequencing data analysis (10 points). [[BISC_219: Assignment Help- Sequence Analysis]]<br> Due in lab next week.<br>
Read for next lab:  Apse, G.P., Aharon, G.S., Snedden, W.A., Blumwald, E. "Salt Tolerance Conferred by Overexpression of a Vacuolar Na+/H+ Antiport in Arabidopsis" ''Science'' (1999) 285: 1256-1258. Available at: http://www.sciencemag.org/cgi/content/full/285/5431/1256 or in pdf form at http://0-www.sciencemag.org.luna.wellesley.edu:80/cgi/reprint/285/5431/1256.pdf and the ''Science'' perspectives article by Frommer ''Taking Transgenic Plants with a Pinch of Salt, '' Science 20 August 1999: Vol. 285. no. 5431, pp. 1222 - 1223. DOI: 10.1126/science.285.5431.1222 at [http://0-www.sciencemag.org.luna.wellesley.edu/cgi/content/full/285/5431/1222]
<br>
and Uddin MI et al., "Overexpression of a New Rice Vacuolar Antiporter Regulating Protein OsARP Improves Salt Tolerance in Tobacco". ''Plant and Cell Physiology'' (2008), Volume 49, Number 6 Pp. 880-890. Available at: http://pcp.oxfordjournals.org/cgi/content/full/49/6/880 <br>
and a Feb. 5, 2002 Boston Globe'' article by ME Malone, ''SCIENTISTS FOCUS ON THE TOBACCO PLANT AS A POSSIBLE CANCER-FIGHTER GENETICALLY ALTERED CROPS MAY SOMEDAY PRODUCE DRUGS TO COMBAT MANY DISEASES''. Available from Lexus/Nexus Academic http://0-www.lexisnexis.com.luna.wellesley.edu/us/lnacademic/search/flap.do?flapID=news&random=0.7807457835138732
<br><br> Work on Paper #2 – Full Paper – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References – [[BISC_219: Assignment Help- RNAi full paper]]- DUE  Nov. 17th at 5 pm
|-
! 9
| Wed. Nov. 11 to <br> Tues. Nov. 17
| '''Plants''': Restriction enzyme digest of PCR reactions <br><br> Agarose gel electrophoresis of cut PCR product<br><br> Paper Discussion #3
|  _
| Work on Paper #2 – Full Paper – Title, Abstract, Intro, Materials and Methods, Results, Discussion, References – [[BISC_219: Assignment Help- RNAi full paper]]- DUE  Nov. 17th at 5 pm
|-
! 10
| Wed. Nov. 18 to <br> Tues. Nov. 24
| '''Plants:'''Spectrophotometric GUS enzyme activity assay <br><br> Phenotypic analysis of plants<br><br> Paper #2 review returned in lecture 11/24 for revision<br>Discuss ''The Science of Science Writing'' by Gopen and Swan from the ''American Scientist'' (Nov.-Dec. 1990, Vol78, 550-558 (Available in the E-Reserves folder on the BISC219 course First Class) conference
| _
| '''Homework''': Analyze all data & create figures/tables from course ''gusA'' and GUS data for the Workshop. Assignment help at [[BISC 219/2009: Mod 3 Analyzing the data on the putative transgenic and control plants]]<br>
<br><br> Work on revision of Paper #2 for resubmission by 12/2 at 5 pm<br>
|-
! 11
| Mon. Nov. 30 to<br> Fri. Dec 4
| '''Data Analysis''':Effective Figure Design Workshop
| _
| '''Homework''': Write final paper on Genetic Engineering in ''Science'' style'''. Due: December 11, 2009 by 5 pm'''. Instructions at [[BISC 219: Assignment Help- Transgenic plants research report ]]
|-
! 12
| Mon. Dec.7 to <br> Fri. Dec. 11
| TBA
| TBA
| TBA
|-
|-
|}
|}
<br>
<br>
</div>

Latest revision as of 09:15, 12 January 2010

Wellesley College     BISC 220     Cellular Physiology

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BISC220 S10 Lab Calendar


Monday Tuesday Wednesday Thursday Friday
Jan. 25

Jan. 26
Lab 1
Enzymes 1 		Jan. 27
Lab 1
Enzymes 1 		Jan. 28
Lab 1
Enzymes 1 		Jan. 29         

Feb. 1

 Feb. 2
Lab 2
Enzymes 2 		Feb. 3
Lab 2
Enzymes 2 		Feb. 4
Lab 2
Enzymes 2 		Feb. 5         

Feb. 8

Feb. 9
Lab 3
Enzymes 3 		Feb. 10
Lab 3
Enzymes 3 		Feb. 11
Lab 3
Enzymes 3 		Feb. 12         

Feb. 15
President's Day
 Feb. 16
Monday Schedule
NO LAB 		Feb. 17
NO LAB
Feb. 18
NO LAB
Feb. 19         

Feb. 22

Feb. 23
Lab 4
Enzymes 4 		Feb. 24
Lab 4
Enzymes 4 		Feb. 25
Lab 4
Enzymes 4 		Feb. 26         

Mar. 1

Mar. 2
Lab 5
Science Writing 		Mar. 3
Lab 5
Science Writing 		Mar. 4
Lab 5
Science Writing 		Mar. 5         

Mar. 8

Mar. 9
Lab 6
Yeast 1
Paper 1 due 		Mar. 10
Lab 6
Yeast 1
Paper 1 due 		Mar. 11
Lab 6
Yeast 1
Paper 1 due 		Mar. 12         

Mar. 15

Mar. 16
Lab 7
Yeast 2 		Mar. 17
Lab 7
Yeast 2 		Mar. 18
Lab 7
Yeast 2 		Mar. 19         

Mar. 22
Spring Break 		Mar. 23
Spring Break 		Mar. 24
Spring Break 		Mar. 25
Spring Break 		Mar. 26         
Spring Break
Mar. 29

Mar. 30
Lab 8
Yeast 3 		Mar. 31
Lab 8
Yeast 3 		April 1
Lab 8
Yeast 3 		April 2         

April 5

April 6
Lab 9
Cell Culture 		April 7
Lab 9
Cell Culture 		April 8
Lab 9
Cell Culture 		April 9         

April 12
April 13
Lab 10
Cytoskeleton 1
Paper 2 due 		April 14
Lab 10
Cytoskeleton 1
Paper 2 due 		April 15
Lab 10
Cytoskeleton 1
Paper 2 due 		April 16

April 19
Patriot's Day 		April 20
Lab 11
Cytoskeleton 2 		April 21
Lab 11
Cytoskeleton 2 		April 22
Monday Schedule 		April 23         

April 26

April 27
Lab 12
Presentations 		April 28
Rhulman
NO LAB 		April 29
Lab 12
Presentations 		April 30         

May 3

May 4
NO LAB 		May 5
Lab 12
Presentations 		May 6
Last day of classes
Paper 3 due 		May 7         


Schedule of Experiments

Series Title Lab #
1 Enzyme Structure/Function 1-5
2 Yeast Genetics and the Secretory Pathway 6-8
3 Cell Culture and the Cytoskeleton 9-12



Graded Assignments

Lab Assigned Lab Due Assignment
description		 Point Value
(Total 200)
Lab 1 Lab 2 RasMol/ClustalW 10
Lab 2 Lab 3 Calculations from Lab 2 10
Lab 3 Lab 4 Calculations from Lab 3 15
Lab 4 Lab 6
Week of March 8th 		β-galactosidase paper 40
Lab 6 Lab 7 Yeast growth predictions/BLAST 15
Lab 8 Lab 10
Week of April 12 		Yeast Secretory Pathway Paper 45
Lab 10 Lab 12 Cytoskeleton Imaging Oral Presentations 25
Lab 11 May 6 - Last Day of Classes Cytoskeleton Partial Paper 35
Other "P" Points - Participation and Preparation 5
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