BISC220 Cell Physiology
Lecturer: Jennifer Hood-DeGrenier
Lab Instructor: Melissa Beers
Lab Prep : Padma Kannabiran
Study Group Leader/ Course Peer Mentor:
Jennifer Hood-DeGrenier: Office SC376A ; office phone x3313; email: @wellesley.edu
Office Hours: Tues. 1:00-2:30 pm or by appointment (SC376A)
Melissa Beers: Office SC175; office phone X3521; email: firstname.lastname@example.org
Office Hours: by arrangement (SC175)
Lectures: Tuesdays & Fridays 11:10-12:20 SC396
Labs: : Tues. 12:30-4; Wed. 2:15-5:45; Thurs. 1-4:30 SC304
Text: Alberts, B. et al. (2008) Molecular Biology of the Cell, 5th ed., Garland Science
Reserve texts and Problem Books:
Lodish, H. et al. (2008) Molecular Cell Biology, 6th ed.
Alberts, B. et al. (2008) Molecular Biology of the Cell, 5th ed.
Wilson, J. and Hunt T. (2002) Molecular Biology of the Cell: A Problems Approach, 4th ed.
Reference material for labs: The lab manual is published electronically at: http://openwetware.org/wiki/BISC220/S10
In the interest of conservation, please do not print out the entire lab manual. Copies will be available for your use in the lab. Notes about the procedures prior to or during the labs should be made in your lab notebook.
Additional lab resource material is posted to the First Class lab conferences for BISC220
By the end of this course, you should be familiar with many of the essential physiological processes of the cell and have a strong understanding of how proteins collaborate to carry out those processes. You will also have acquired a “methods tool-kit” and an understanding of how to apply various experimental techniques to answer cell biological questions. Finally, you should have gained confidence in reading and interpreting primary-source scientific articles enhanced your scientific writing abilities.
| Assessment || Points
| Hour Exams
|| 200 points
| Final Exam
|| 140 points
|| 60 points
|| 200 points
| Total || 600 points
POLICY ON LATE ASSIGNMENTS AND MAKE-UP EXAMS:
Make-up exams and quizzes will only be given in the case of a serious personal illness or family emergency that has been documented by your class dean. Similarly, excused extensions for lab reports or homework will not be granted except in the cases described. In such situations, if at all possible, the instructor should be notified prior to the class period in which the exam or quiz is to be administered or when the assignment is due. Unexcused late work will be penalized 5% per day and will not receive credit more than one week after the due date. Students who have a legitimate conflict with a scheduled exam are requested to speak with the instructor as soon as possible about taking the exam early, but no more than 48 hours in advanced of the scheduled exam.
STUDENTS WITH SPECIAL NEEDS:
Students entitled to disability-related accommodations are requested to speak with the instructor about their needs well in advance of scheduled quizzes, exams, and assignment due dates.
POLICY ON COLLABORATION:
Working in groups with your classmates is encouraged as a valuable way to enhance your understanding of the course material; however, the actual writing of all assignments, including homework and lab reports, must be completed individually.
POTENTIAL CHANGES TO THE SYLLABUS:
The instructor reserves the right to modify the syllabus during the semester as she deems necessary to meet the course goals in a reasonable manner.
Please let your instructors know if you are having trouble during the semester. The sooner you bring a problem to our attention, the sooner we can help you address it.
BISC220 Lecture Schedule
| Lec. # || Date || Topics || Reading
|| T 1/26
|| Intro: The AMAZING World of the Cell |
| Review Ch. 1 & Ch. 2 (general background, review from BISC 110) |
Ch. 3, p. 125-148
Ch. 8, p. 527-530 (protein structure determination)
http://www.youtube.com/watch?v=BtZEqQ1cpmk (Harvard BioVisions)
View Cell Biology Interactive (CBI) videos 3.1-3.6, 3.9 (DVD that came with your textbook—see conference for access notes)
|| F 1/29
|| Protein Regulation & Social Context |
Enzymes I: Mechanism of Catalysis
| Ch. 2, p. 72-81 |
Ch. 3, p. 152-161, 164-167 & 175-190
CBI videos 3.7, 3.8 & 3.11
Ringe D, Petsko G A (2008) How enzymes work. Science 320:1428-1429.*
|| T 2/2
|| Enzymes II: Kinetics & Regulation |
Quiz: Amino Acids (10 pts)
| Ch. 3 p. 162-163 & 166-174 |
CBI video 3.10
Biology Hypertextbook Enzyme Biochemistry http://www.sciencegateway.org/resources/biologytext/index.html sections 3 & 4 (also try practice problems #1-3)
|| F 2/5
|| Protein Methods I: Protein Separation & Antibody Detection
|| Ch. 8, p. 508-509 & 517-522 |
Ch. 25, p. 1551-1561 (on the textbook DVD)
CBI video 25.2
|| T 2/9
|| Protein Methods II: Protein Purification & Functional Analysis
|| Ch. 8, p. 510-516, 522-531 & 564-576 |
Snyder M and Gallagher JEG (2009) Systems biology from a yeast omics perspective. FEBS Letters 583:3895-3899.*
|| F 2/12