BISC220/S10: Lecture Syllabus
BISC220 Cell Physiology
Lecturer: Jennifer Hood-DeGrenier
Lab Instructor: Melissa Beers
Lab Prep : Padma Kannabiran
Study Group Leader/ Course Peer Mentor:
Contact Info:
Jennifer Hood-DeGrenier: Office SC376A ; office phone x3313; email: @wellesley.edu
Office Hours: Tues. 1:00-2:30 pm or by appointment (SC376A)
Melissa Beers: Office SC175; office phone X3521; email: mbeers@wellesley.edu
Office Hours: by arrangement (SC175)
Lectures: Tuesdays & Fridays 11:10-12:20 SC396
Labs: : Tues. 12:30-4; Wed. 2:15-5:45; Thurs. 1-4:30 SC304
Text: Alberts, B. et al. (2008) Molecular Biology of the Cell, 5th ed., Garland Science
Reserve texts and Problem Books:
Lodish, H. et al. (2008) Molecular Cell Biology, 6th ed.
Alberts, B. et al. (2008) Molecular Biology of the Cell, 5th ed.
Wilson, J. and Hunt T. (2002) Molecular Biology of the Cell: A Problems Approach, 4th ed.
Reference material for labs: The lab manual is published electronically at: http://openwetware.org/wiki/BISC220/S10
In the interest of conservation, please do not print out the entire lab manual. Copies will be available for your use in the lab. Notes about the procedures prior to or during the labs should be made in your lab notebook.
Additional lab resource material is posted to the First Class lab conferences for BISC220
EXPECTED OUTCOMES:
By the end of this course, you should be familiar with many of the essential physiological processes of the cell and have a strong understanding of how proteins collaborate to carry out those processes. You will also have acquired a “methods tool-kit” and an understanding of how to apply various experimental techniques to answer cell biological questions. Finally, you should have gained confidence in reading and interpreting primary-source scientific articles enhanced your scientific writing abilities.
GRADING:
| Assessment | Points |
|---|---|
| Hour Exams | 200 points |
| Final Exam | 140 points |
| Homework/Quizzes | 60 points |
| Laboratory | 200 points |
| Total | 600 points |
POLICY ON LATE ASSIGNMENTS AND MAKE-UP EXAMS:
Make-up exams and quizzes will only be given in the case of a serious personal illness or family emergency that has been documented by your class dean. Similarly, excused extensions for lab reports or homework will not be granted except in the cases described. In such situations, if at all possible, the instructor should be notified prior to the class period in which the exam or quiz is to be administered or when the assignment is due. Unexcused late work will be penalized 5% per day and will not receive credit more than one week after the due date. Students who have a legitimate conflict with a scheduled exam are requested to speak with the instructor as soon as possible about taking the exam early, but no more than 48 hours in advanced of the scheduled exam.
STUDENTS WITH SPECIAL NEEDS:
Students entitled to disability-related accommodations are requested to speak with the instructor about their needs well in advance of scheduled quizzes, exams, and assignment due dates.
POLICY ON COLLABORATION:
Working in groups with your classmates is encouraged as a valuable way to enhance your understanding of the course material; however, the actual writing of all assignments, including homework and lab reports, must be completed individually.
POTENTIAL CHANGES TO THE SYLLABUS:
The instructor reserves the right to modify the syllabus during the semester as she deems necessary to meet the course goals in a reasonable manner.
Please let your instructors know if you are having trouble during the semester. The sooner you bring a problem to our attention, the sooner we can help you address it.
BISC220 Lecture Schedule
| Lec. # | Date | Topics | Reading |
|---|---|---|---|
| 1 | T 1/26 | Intro: The AMAZING World of the Cell Protein Structure |
Review Ch. 1 & Ch. 2 (general background, review from BISC 110) Ch. 3, p. 125-148 Ch. 8, p. 527-530 (protein structure determination) |
| 2 | F 1/29 | Protein Regulation & Social Context Enzymes I: Mechanism of Catalysis |
Ch. 2, p. 72-81 Ch. 3, p. 152-161, 164-167 & 175-190 CBI videos 3.7, 3.8 & 3.11 |
| 3 | T 2/2 | Enzymes II: Kinetics & Regulation Quiz: Amino Acids (10 pts) |
Ch. 3 p. 162-163 & 166-174 CBI video 3.10 Biology Hypertextbook Enzyme Biochemistry http://www.sciencegateway.org/resources/biologytext/index.html sections 3 & 4 (also try practice problems #1-3) |
| 4 | F 2/5 | Protein Methods I: Protein Separation & Antibody Detection | Ch. 8, p. 508-509 & 517-522 Ch. 25, p. 1551-1561 (on the textbook DVD) CBI video 25.2 |
| 5 | T 2/9 | Protein Methods II: Protein Purification & Functional Analysis | Ch. 8, p. 510-516, 522-531 & 564-576 Snyder M and Gallagher JEG (2009) Systems biology from a yeast omics perspective. FEBS Letters 583:3895-3899.* |
| 6 | F 2/12 | The Life Cycle of a Protein: Folding to Destruction | Ch. 6 p. 387-399 Kolata G (1986) New Rule Proposed for Protein Degradation. Science 234:151-152.* |
| T 2/16 | No Class—Monday Schedule | ||
| 7 | F 2/19 | Imaging Cells & Molecules BLAST Homework (#1) Due (10 pts) |
Ch. 9 http://bioeducate.ascb.org/ — check out 2008 ASCB “Celldance” Contest winning videos |
| 8 | T 2/23 | Membranes & Membrane Proteins | Ch. 10 CBI videos 10.1-10.6 |
| F 2/26 | EXAM I (100 pts) | ||
| 9 | T 3/2 | Membrane Transport & Electrical Properties of Membranes | Ch. 11 p. 651-683 CBI videos 11.1-11.6 & 15.1 Doyle DA et al. (1998) The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity. Science 280:69-77.* |
| 10 | F 3/5 | Protein Sorting: Principles & Experimental Approaches Protein Insertion into the Endoplasmic Reticulum |
Ch. 12 p. 695-704 & 723-738 CBI videos 12.1, 12.4, & 12.6 |
| 11 | T 3/9 | ER Quality Control & Principles of Vesicle Trafficking | Ch. 12, p. 738-742 Ch. 13, p. 749-766 CBI videos 13.1 & 13.4 Pucadyil TJ, Schmid S (2009) Conserved Functions of Membrane Active GTPases in Coated Vesicle Formation. Science 325:1217-1220.* |
| 12 | F 3/12 | Intra- and Trans-Cellular Vesicle Trafficking Ion Channel Homework (#2) Due (10 pts) |
Ch. 13, p. 766-809 CBI videos 13.2, 13.3, 13.5 & 13.6 |
| 13 | T 3/16 | Nucleocytoplasmic Transport | Ch. 12, p. 704-710 CBI videos 12.2 & 12.5 Weis K (2007) The Nuclear Pore Complex: Oily Spaghetti or Gummy Bear? Cell 130:405-407.* |
| 14 | F 3/19 | Workshop: Nuclear Transport in STAT1 Transcription Factor Function | McBride KM, Banninger G, McDonald C, Reich, NC (2002) Regulated nuclear import of the STAT1 transcription factor by direct binding of importin-α. EMBO J. 21:1754-1763.* McBride KM, Reich NC (2003) The ins and outs of STAT1 nuclear transport. Sci STKE 195:RE13* |
| 3/23 & 3/26 | SPRING BREAK | ||
| 15 | T 3/30 | Cell Signaling I: Overview/ RTKs & Ras-MAPK Pathway | Ch. 15, p. 879-903 & 921-930 CBI videos 15.4 & 15.5 |
