BISC220/S10: Media Recipes: Difference between revisions

Latest revision as of 17:01, 2 March 2010

Wellesley College BISC 220 Cellular Physiology

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Enzymes Secretory Pathway Cytoskeleton OWW Basics


Solution	Composition
PBS (Phosphate Buffered Saline) 1X	2.68 mM KCl 1.46 mM KH₂PO₄ 136.9 NaCl 8.1 mM Na₂HPO₄
PBST (PBS with Tween 20)	1X PBS with 0.25% Tween 20
Trypsin	Stock = 0.25%
PEM Buffer	1 M Pipes 0.05 M EGTA 0.02 M MgCl₂ 6H₂O
Cell Culture Media	1 Packet DMEM Powder 0.15% NaHCO₃ 10% Newborn Calf Serum
Trypan Blue	0.1% Trypan Blue in water

@@ Line 3: / Line 3: @@
-== Yeast Secretory Pathway Series Media Recipes ==
+== Cytoskeleton Series Media Recipes ==
 {| border="1"
 |+
 ! Solution !! Composition
-|-
-! Yeast Sample Buffer 5X
-| 10% SDS <br> 25% glycerol <br> 250 mM Tris (pH 6.8) <br> 500 mM DTT <br> 0.5% BPB
-|-
-! Gel Running Buffer
-| 0.92 M Glycine <br> 0.05 M Trisma Base <br> 0.1% SDS
-|-
-! Blotting Buffer (Western Transfer Buffer)
-| 25 mM Trisma Base <br> 192 mM Glycine <br> 20% v/v Methanol
 |-
 ! PBS (Phosphate Buffered Saline) 1X
@@ Line 23: / Line 14: @@
 | 1X PBS with 0.25% Tween 20
 |-
-! Blocking Buffer
+! Trypsin
-| 5% Milk Powder in PBST
+| Stock = 0.25%
 |-
-! SD -Ura Media <br> Sabouraud's Dextrose
+! PEM Buffer
-| 0.67% Yeast Nitrogen Base <br> 2% glucose <br> 0.08% -Ura amino acid dropout mix <br> 2% agar (only in plates)
+| 1 M Pipes <br> 0.05 M EGTA <br> 0.02 M MgCl<sub>2</sub> 6H<sub>2</sub>O <br>
 |-
-! SD -Ura -His Media
+! Cell Culture Media
-| 0.67% Yeast Nitrogen Base <br> 2% glucose <br> 0.078% -Ura -His amino acid dropout mix <br> 2% agar (only in plates) <br> 3 mM Histidinol
+| 1 Packet DMEM Powder <br> 0.15% NaHCO<sub>3</sub> <br> 10% Newborn Calf Serum
 |-
-! YPD (Yeast Peptone Dextrose) Media
+! Trypan Blue
-| 1% Yeast Extract <br> 2% Peptone <br> 2% glucose <br> 2% Agar (only in plates)
+| 0.1% Trypan Blue in water
 |-
 |}